Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters

Autores
Andrada, Lidia Estefania; Márquez, María Antonela; Chagra Dib, Elsa Patricia; Gauffin Cano, María Paola; Medina, Roxana Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corn stover (CS) is an abundant lignocellulosic by-product of the grain industry. Ferulic acid esterase producing (FAE+)-lactobacilli can potentially improve ensiled forages’ nutritive value through the hydrolysis of ferulic acid ester bonds present in cell walls during the fermentation process, but this has not been addressed in CS silage. In this study, we characterized 8 FAE+ lactobacilli regarding their FAE activity and inoculant aptitude: Lactobacillus (L.) johnsonii (CRL2237, CRL2238, CRL2240), L. plantarum (ETC182, CRL046, CRL2241), L. fermentum CRL1446 and L. brevis CRL2239. Next, 25% dry matter (DM) CS mini silos were prepared and either not inoculated (UN) or inoculated with each strain (105 CFU g fresh matter−1). Compared to UN, DM loss was significantly reduced in CRL046 and CRL2239, and organic matter increased in CRL2241-inoculated silages. Although the rest of the digestibility measures were not improved, in situ acid detergent fiber degradability (ADFD) was increased by the CRL2238 strain when compared to UN. Results in inoculated silages were not correlated with FAE activity quantification or growth/acidification studies in a CS-derived culture broth. This study demonstrates the potential of several FAE+ lactobacilli strains as CS inoculants and encourages further research.
Fil: Andrada, Lidia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Márquez, María Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chagra Dib, Elsa Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina
Fil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Materia
FERULOYL ESTERASES
LACTIC ACID BACTERIA
RUMINANT NUTRITION
SILAGE INOCULANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/249552
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/249552
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parametersAndrada, Lidia EstefaniaMárquez, María AntonelaChagra Dib, Elsa PatriciaGauffin Cano, María PaolaMedina, Roxana BeatrizFERULOYL ESTERASESLACTIC ACID BACTERIARUMINANT NUTRITIONSILAGE INOCULANTShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Corn stover (CS) is an abundant lignocellulosic by-product of the grain industry. Ferulic acid esterase producing (FAE+)-lactobacilli can potentially improve ensiled forages’ nutritive value through the hydrolysis of ferulic acid ester bonds present in cell walls during the fermentation process, but this has not been addressed in CS silage. In this study, we characterized 8 FAE+ lactobacilli regarding their FAE activity and inoculant aptitude: Lactobacillus (L.) johnsonii (CRL2237, CRL2238, CRL2240), L. plantarum (ETC182, CRL046, CRL2241), L. fermentum CRL1446 and L. brevis CRL2239. Next, 25% dry matter (DM) CS mini silos were prepared and either not inoculated (UN) or inoculated with each strain (105 CFU g fresh matter−1). Compared to UN, DM loss was significantly reduced in CRL046 and CRL2239, and organic matter increased in CRL2241-inoculated silages. Although the rest of the digestibility measures were not improved, in situ acid detergent fiber degradability (ADFD) was increased by the CRL2238 strain when compared to UN. Results in inoculated silages were not correlated with FAE activity quantification or growth/acidification studies in a CS-derived culture broth. This study demonstrates the potential of several FAE+ lactobacilli strains as CS inoculants and encourages further research.Fil: Andrada, Lidia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Márquez, María Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Chagra Dib, Elsa Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; ArgentinaFil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaMolecular Diversity Preservation International2023-03-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249552Andrada, Lidia Estefania; Márquez, María Antonela; Chagra Dib, Elsa Patricia; Gauffin Cano, María Paola; Medina, Roxana Beatriz; Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters; Molecular Diversity Preservation International; Fermentation; 9; 4; 27-3-2023; 1-152311-5637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/4/331info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9040331info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:57Zoai:ri.conicet.gov.ar:11336/249552instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:58.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
title Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
spellingShingle Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
Andrada, Lidia Estefania
FERULOYL ESTERASES
LACTIC ACID BACTERIA
RUMINANT NUTRITION
SILAGE INOCULANTS
title_short Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
title_full Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
title_fullStr Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
title_full_unstemmed Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
title_sort Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters
dc.creator.none.fl_str_mv Andrada, Lidia Estefania
Márquez, María Antonela
Chagra Dib, Elsa Patricia
Gauffin Cano, María Paola
Medina, Roxana Beatriz
author Andrada, Lidia Estefania
author_facet Andrada, Lidia Estefania
Márquez, María Antonela
Chagra Dib, Elsa Patricia
Gauffin Cano, María Paola
Medina, Roxana Beatriz
author_role author
author2 Márquez, María Antonela
Chagra Dib, Elsa Patricia
Gauffin Cano, María Paola
Medina, Roxana Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv FERULOYL ESTERASES
LACTIC ACID BACTERIA
RUMINANT NUTRITION
SILAGE INOCULANTS
topic FERULOYL ESTERASES
LACTIC ACID BACTERIA
RUMINANT NUTRITION
SILAGE INOCULANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Corn stover (CS) is an abundant lignocellulosic by-product of the grain industry. Ferulic acid esterase producing (FAE+)-lactobacilli can potentially improve ensiled forages’ nutritive value through the hydrolysis of ferulic acid ester bonds present in cell walls during the fermentation process, but this has not been addressed in CS silage. In this study, we characterized 8 FAE+ lactobacilli regarding their FAE activity and inoculant aptitude: Lactobacillus (L.) johnsonii (CRL2237, CRL2238, CRL2240), L. plantarum (ETC182, CRL046, CRL2241), L. fermentum CRL1446 and L. brevis CRL2239. Next, 25% dry matter (DM) CS mini silos were prepared and either not inoculated (UN) or inoculated with each strain (105 CFU g fresh matter−1). Compared to UN, DM loss was significantly reduced in CRL046 and CRL2239, and organic matter increased in CRL2241-inoculated silages. Although the rest of the digestibility measures were not improved, in situ acid detergent fiber degradability (ADFD) was increased by the CRL2238 strain when compared to UN. Results in inoculated silages were not correlated with FAE activity quantification or growth/acidification studies in a CS-derived culture broth. This study demonstrates the potential of several FAE+ lactobacilli strains as CS inoculants and encourages further research.
Fil: Andrada, Lidia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Márquez, María Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Chagra Dib, Elsa Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina
Fil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description Corn stover (CS) is an abundant lignocellulosic by-product of the grain industry. Ferulic acid esterase producing (FAE+)-lactobacilli can potentially improve ensiled forages’ nutritive value through the hydrolysis of ferulic acid ester bonds present in cell walls during the fermentation process, but this has not been addressed in CS silage. In this study, we characterized 8 FAE+ lactobacilli regarding their FAE activity and inoculant aptitude: Lactobacillus (L.) johnsonii (CRL2237, CRL2238, CRL2240), L. plantarum (ETC182, CRL046, CRL2241), L. fermentum CRL1446 and L. brevis CRL2239. Next, 25% dry matter (DM) CS mini silos were prepared and either not inoculated (UN) or inoculated with each strain (105 CFU g fresh matter−1). Compared to UN, DM loss was significantly reduced in CRL046 and CRL2239, and organic matter increased in CRL2241-inoculated silages. Although the rest of the digestibility measures were not improved, in situ acid detergent fiber degradability (ADFD) was increased by the CRL2238 strain when compared to UN. Results in inoculated silages were not correlated with FAE activity quantification or growth/acidification studies in a CS-derived culture broth. This study demonstrates the potential of several FAE+ lactobacilli strains as CS inoculants and encourages further research.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/249552
Andrada, Lidia Estefania; Márquez, María Antonela; Chagra Dib, Elsa Patricia; Gauffin Cano, María Paola; Medina, Roxana Beatriz; Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters; Molecular Diversity Preservation International; Fermentation; 9; 4; 27-3-2023; 1-15
2311-5637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/249552
identifier_str_mv Andrada, Lidia Estefania; Márquez, María Antonela; Chagra Dib, Elsa Patricia; Gauffin Cano, María Paola; Medina, Roxana Beatriz; Corn stover silage inoculated with ferulic acid esterase producing L. johnsonii, L. plantarum, L. fermentum, and L. brevis Strains: fermentative and nutritional parameters; Molecular Diversity Preservation International; Fermentation; 9; 4; 27-3-2023; 1-15
2311-5637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/4/331
info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9040331
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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