Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype

Autores
Sidabra, Johanna Elena; Capobianco, Carla Sabrina; Gottardo, María Florencia; Alonso, Daniel Fernando; Farina, Hernán Gabriel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.
Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Nanomedicinas
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Virología
Materia
BREAST CANCER
MINIMAL RESIDUAL DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131800

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotypeSidabra, Johanna ElenaCapobianco, Carla SabrinaGottardo, María FlorenciaAlonso, Daniel FernandoFarina, Hernán GabrielBREAST CANCERMINIMAL RESIDUAL DISEASEhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaSociedad Argentina de NanomedicinasSociedad Argentina de Investigación ClínicaSociedad Argentina de VirologíaFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131800Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-10025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:19Zoai:ri.conicet.gov.ar:11336/131800instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:19.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
title Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
spellingShingle Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
Sidabra, Johanna Elena
BREAST CANCER
MINIMAL RESIDUAL DISEASE
title_short Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
title_full Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
title_fullStr Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
title_full_unstemmed Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
title_sort Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
dc.creator.none.fl_str_mv Sidabra, Johanna Elena
Capobianco, Carla Sabrina
Gottardo, María Florencia
Alonso, Daniel Fernando
Farina, Hernán Gabriel
author Sidabra, Johanna Elena
author_facet Sidabra, Johanna Elena
Capobianco, Carla Sabrina
Gottardo, María Florencia
Alonso, Daniel Fernando
Farina, Hernán Gabriel
author_role author
author2 Capobianco, Carla Sabrina
Gottardo, María Florencia
Alonso, Daniel Fernando
Farina, Hernán Gabriel
author2_role author
author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
MINIMAL RESIDUAL DISEASE
topic BREAST CANCER
MINIMAL RESIDUAL DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.
Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Nanomedicinas
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Virología
description Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131800
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-1
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131800
identifier_str_mv Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-1
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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