Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype
- Autores
- Sidabra, Johanna Elena; Capobianco, Carla Sabrina; Gottardo, María Florencia; Alonso, Daniel Fernando; Farina, Hernán Gabriel
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.
Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Nanomedicinas
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Virología - Materia
-
BREAST CANCER
MINIMAL RESIDUAL DISEASE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/131800
Ver los metadatos del registro completo
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Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotypeSidabra, Johanna ElenaCapobianco, Carla SabrinaGottardo, María FlorenciaAlonso, Daniel FernandoFarina, Hernán GabrielBREAST CANCERMINIMAL RESIDUAL DISEASEhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process.Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaSociedad Argentina de NanomedicinasSociedad Argentina de Investigación ClínicaSociedad Argentina de VirologíaFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131800Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-10025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:19Zoai:ri.conicet.gov.ar:11336/131800instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:19.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
title |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
spellingShingle |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype Sidabra, Johanna Elena BREAST CANCER MINIMAL RESIDUAL DISEASE |
title_short |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
title_full |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
title_fullStr |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
title_full_unstemmed |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
title_sort |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype |
dc.creator.none.fl_str_mv |
Sidabra, Johanna Elena Capobianco, Carla Sabrina Gottardo, María Florencia Alonso, Daniel Fernando Farina, Hernán Gabriel |
author |
Sidabra, Johanna Elena |
author_facet |
Sidabra, Johanna Elena Capobianco, Carla Sabrina Gottardo, María Florencia Alonso, Daniel Fernando Farina, Hernán Gabriel |
author_role |
author |
author2 |
Capobianco, Carla Sabrina Gottardo, María Florencia Alonso, Daniel Fernando Farina, Hernán Gabriel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
BREAST CANCER MINIMAL RESIDUAL DISEASE |
topic |
BREAST CANCER MINIMAL RESIDUAL DISEASE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.5 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process. Fil: Sidabra, Johanna Elena. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capobianco, Carla Sabrina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gottardo, María Florencia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología Argentina Sociedad Argentina de Inmunología Sociedad Argentina de Fisiología Sociedad Argentina de Nanomedicinas Sociedad Argentina de Investigación Clínica Sociedad Argentina de Virología |
description |
Breast cancer is the first cause of death from female cancer. The recurrence of the disease originated at the level of secondary organs, or metastasis, is responsible for 90% of deaths from cancer. The factors that endow these cells with metastatic functions are largely unknown. One of the limitations in the study of tumor cells with metastatic phenotypes is that cell lines maintained in culture lose this ability to invade and colonize tissues. On the other hand, it has been shown that reinjection of cells in animals can lead to their enrichment with aggressive phenotypes. The aim of this work was the isolation and characterization of different cell populations with differentiated metastatic capacities. Following inoculation of the F3II murine mammary carcinoma cell lines, we established cell populations in vitro, one from the primary tumor and another from a metastatic nodule, F3II TP and F3II NM cell lines respectively. To determine their aggressiveness, a series of additional characteristics were compared between these lines and F3II. The three lines showed variations in morphology in culture and a different doubling time, with F3II NM having the highest one. Moreover, F3II NM presented major adhesion capacity and lower clonogenic potential. This could be explained by the differential expression of cell adhesion molecules, such as integrins or cadherins analyzed by flow cytometry. In addition, the migration capacity was analyzed by transwell assay and the results showed differences in this process. Finally, we compared the behavior in vivo and we detected variations in tumor progression such as latency, frequency of ulceration, tumor growth and the presence of pulmonary nodules. All things considered, the establishment and characterization of these two new different cell lines with differentiated metastatic capacities will allow us to determine molecular differences involved in the metastatic process. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/131800 Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-1 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/131800 |
identifier_str_mv |
Isolation and characterization of murine mammary cell lines with differentiated aggressive phenotype; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología ; Argentina; 2018; 1-1 0025-7680 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Fundación Revista Medicina |
publisher.none.fl_str_mv |
Fundación Revista Medicina |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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