The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach

Autores
Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF.
Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina
Fil: Fernandez, Daniel Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina
Fil: Vanella, Fabián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Chalde, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Materia
ALIEN INVASIVE SPECIES
EDNA
NON-NATIVE SPECIES
SALMONIDS
SOUTH AMERICA
TIERRA DEL FUEGO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147676
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147676
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approachNardi, Cristina FernandaFernandez, Daniel AlfredoVanella, Fabián AlbertoChalde, TomásALIEN INVASIVE SPECIESEDNANON-NATIVE SPECIESSALMONIDSSOUTH AMERICATIERRA DEL FUEGOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF.Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; ArgentinaFil: Fernandez, Daniel Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; ArgentinaFil: Vanella, Fabián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Chalde, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaSpringer2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147676Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás; The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach; Springer; Biological Invasions; 21; 4; 1-2019; 1415-14251387-3547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10530-018-1908-8info:eu-repo/semantics/altIdentifier/doi/10.1007/S10530-018-1908-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:55Zoai:ri.conicet.gov.ar:11336/147676instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:55.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
title The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
spellingShingle The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
Nardi, Cristina Fernanda
ALIEN INVASIVE SPECIES
EDNA
NON-NATIVE SPECIES
SALMONIDS
SOUTH AMERICA
TIERRA DEL FUEGO
title_short The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
title_full The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
title_fullStr The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
title_full_unstemmed The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
title_sort The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
dc.creator.none.fl_str_mv Nardi, Cristina Fernanda
Fernandez, Daniel Alfredo
Vanella, Fabián Alberto
Chalde, Tomás
author Nardi, Cristina Fernanda
author_facet Nardi, Cristina Fernanda
Fernandez, Daniel Alfredo
Vanella, Fabián Alberto
Chalde, Tomás
author_role author
author2 Fernandez, Daniel Alfredo
Vanella, Fabián Alberto
Chalde, Tomás
author2_role author
author
author
dc.subject.none.fl_str_mv ALIEN INVASIVE SPECIES
EDNA
NON-NATIVE SPECIES
SALMONIDS
SOUTH AMERICA
TIERRA DEL FUEGO
topic ALIEN INVASIVE SPECIES
EDNA
NON-NATIVE SPECIES
SALMONIDS
SOUTH AMERICA
TIERRA DEL FUEGO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF.
Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina
Fil: Fernandez, Daniel Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina
Fil: Vanella, Fabián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Chalde, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
description The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147676
Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás; The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach; Springer; Biological Invasions; 21; 4; 1-2019; 1415-1425
1387-3547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147676
identifier_str_mv Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás; The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach; Springer; Biological Invasions; 21; 4; 1-2019; 1415-1425
1387-3547
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10530-018-1908-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/S10530-018-1908-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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