Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility

Autores
Weidinger, S.; Mayerhofer, Artur; Frungieri, Monica Beatriz; Meineke, V.; Ring, J.; Köhn, F. M.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The detection of significant levels of tryptase in human seminal plasma and follicular fluid and of tryptase-positive mast cells (MCs) in the wall of human Fallopian tubes lead us to hypothesize that tryptase may exert regulatory actions on human spermatozoa. Immunoelectronmicroscopy revealed proteinase-activated receptor 2 (PAR-2) in the membranes of the acrosomal region and midpiece of human spermatozoa. These PAR-2 were functional, as exposure of spermatozoa from healthy men (n = 12) with regular standard semen parameters to human recombinant tryptase significantly decreased motility in a dose- and time-dependent fashion. Motile spermatozoa (WHO a + b) were significantly decreased within 10 min of incubation with 1.000 ng/ml tryptase (P = 0.045). After 30 and 60 min, significant reduction of motility was also observed in the presence of lower tryptase concentrations (100 ng/ml, P = 0.037; 10 ng/ml, P = 0.046). The inhibitory effects of tryptase progressed throughout an observation period of 180 min. Furthermore, tryptase effects were reversible after washing procedures and could be inhibited by pretreatment with anti-tryptase antibody or anti-PAR-2 antiserum.
Fil: Weidinger, S.. Technische Universität München; Alemania
Fil: Mayerhofer, Artur. Universität München; Alemania
Fil: Frungieri, Monica Beatriz. Universität München; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Meineke, V.. Military Academy Munich; Alemania
Fil: Ring, J.. Technische Universität München; Alemania
Fil: Köhn, F. M.. Technische Universität München; Alemania
Materia
Fertility
Humano
Sperm
Tryptase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36230
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motilityWeidinger, S.Mayerhofer, ArturFrungieri, Monica BeatrizMeineke, V.Ring, J.Köhn, F. M.FertilityHumanoSpermTryptasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The detection of significant levels of tryptase in human seminal plasma and follicular fluid and of tryptase-positive mast cells (MCs) in the wall of human Fallopian tubes lead us to hypothesize that tryptase may exert regulatory actions on human spermatozoa. Immunoelectronmicroscopy revealed proteinase-activated receptor 2 (PAR-2) in the membranes of the acrosomal region and midpiece of human spermatozoa. These PAR-2 were functional, as exposure of spermatozoa from healthy men (n = 12) with regular standard semen parameters to human recombinant tryptase significantly decreased motility in a dose- and time-dependent fashion. Motile spermatozoa (WHO a + b) were significantly decreased within 10 min of incubation with 1.000 ng/ml tryptase (P = 0.045). After 30 and 60 min, significant reduction of motility was also observed in the presence of lower tryptase concentrations (100 ng/ml, P = 0.037; 10 ng/ml, P = 0.046). The inhibitory effects of tryptase progressed throughout an observation period of 180 min. Furthermore, tryptase effects were reversible after washing procedures and could be inhibited by pretreatment with anti-tryptase antibody or anti-PAR-2 antiserum.Fil: Weidinger, S.. Technische Universität München; AlemaniaFil: Mayerhofer, Artur. Universität München; AlemaniaFil: Frungieri, Monica Beatriz. Universität München; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Meineke, V.. Military Academy Munich; AlemaniaFil: Ring, J.. Technische Universität München; AlemaniaFil: Köhn, F. M.. Technische Universität München; AlemaniaOxford University Press2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36230Weidinger, S.; Mayerhofer, Artur; Frungieri, Monica Beatriz; Meineke, V.; Ring, J.; et al.; Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility; Oxford University Press; Human Reproduction; 18; 12; 2003; 2519-25240268-11611460-2350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/18/12/2519/2913425info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deg476info:eu-repo/semantics/altIdentifier/pmid/14645166info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:32Zoai:ri.conicet.gov.ar:11336/36230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:32.842CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
title Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
spellingShingle Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
Weidinger, S.
Fertility
Humano
Sperm
Tryptase
title_short Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
title_full Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
title_fullStr Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
title_full_unstemmed Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
title_sort Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility
dc.creator.none.fl_str_mv Weidinger, S.
Mayerhofer, Artur
Frungieri, Monica Beatriz
Meineke, V.
Ring, J.
Köhn, F. M.
author Weidinger, S.
author_facet Weidinger, S.
Mayerhofer, Artur
Frungieri, Monica Beatriz
Meineke, V.
Ring, J.
Köhn, F. M.
author_role author
author2 Mayerhofer, Artur
Frungieri, Monica Beatriz
Meineke, V.
Ring, J.
Köhn, F. M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fertility
Humano
Sperm
Tryptase
topic Fertility
Humano
Sperm
Tryptase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The detection of significant levels of tryptase in human seminal plasma and follicular fluid and of tryptase-positive mast cells (MCs) in the wall of human Fallopian tubes lead us to hypothesize that tryptase may exert regulatory actions on human spermatozoa. Immunoelectronmicroscopy revealed proteinase-activated receptor 2 (PAR-2) in the membranes of the acrosomal region and midpiece of human spermatozoa. These PAR-2 were functional, as exposure of spermatozoa from healthy men (n = 12) with regular standard semen parameters to human recombinant tryptase significantly decreased motility in a dose- and time-dependent fashion. Motile spermatozoa (WHO a + b) were significantly decreased within 10 min of incubation with 1.000 ng/ml tryptase (P = 0.045). After 30 and 60 min, significant reduction of motility was also observed in the presence of lower tryptase concentrations (100 ng/ml, P = 0.037; 10 ng/ml, P = 0.046). The inhibitory effects of tryptase progressed throughout an observation period of 180 min. Furthermore, tryptase effects were reversible after washing procedures and could be inhibited by pretreatment with anti-tryptase antibody or anti-PAR-2 antiserum.
Fil: Weidinger, S.. Technische Universität München; Alemania
Fil: Mayerhofer, Artur. Universität München; Alemania
Fil: Frungieri, Monica Beatriz. Universität München; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Meineke, V.. Military Academy Munich; Alemania
Fil: Ring, J.. Technische Universität München; Alemania
Fil: Köhn, F. M.. Technische Universität München; Alemania
description The detection of significant levels of tryptase in human seminal plasma and follicular fluid and of tryptase-positive mast cells (MCs) in the wall of human Fallopian tubes lead us to hypothesize that tryptase may exert regulatory actions on human spermatozoa. Immunoelectronmicroscopy revealed proteinase-activated receptor 2 (PAR-2) in the membranes of the acrosomal region and midpiece of human spermatozoa. These PAR-2 were functional, as exposure of spermatozoa from healthy men (n = 12) with regular standard semen parameters to human recombinant tryptase significantly decreased motility in a dose- and time-dependent fashion. Motile spermatozoa (WHO a + b) were significantly decreased within 10 min of incubation with 1.000 ng/ml tryptase (P = 0.045). After 30 and 60 min, significant reduction of motility was also observed in the presence of lower tryptase concentrations (100 ng/ml, P = 0.037; 10 ng/ml, P = 0.046). The inhibitory effects of tryptase progressed throughout an observation period of 180 min. Furthermore, tryptase effects were reversible after washing procedures and could be inhibited by pretreatment with anti-tryptase antibody or anti-PAR-2 antiserum.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36230
Weidinger, S.; Mayerhofer, Artur; Frungieri, Monica Beatriz; Meineke, V.; Ring, J.; et al.; Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility; Oxford University Press; Human Reproduction; 18; 12; 2003; 2519-2524
0268-1161
1460-2350
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36230
identifier_str_mv Weidinger, S.; Mayerhofer, Artur; Frungieri, Monica Beatriz; Meineke, V.; Ring, J.; et al.; Mast cell-sperm interaction: evidence for tryptase and proteinase-activated receptors in the regulation of sperm motility; Oxford University Press; Human Reproduction; 18; 12; 2003; 2519-2524
0268-1161
1460-2350
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/humrep/article/18/12/2519/2913425
info:eu-repo/semantics/altIdentifier/doi/10.1093/humrep/deg476
info:eu-repo/semantics/altIdentifier/pmid/14645166
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082978505031680
score 12.891075

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