Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation

Autores
Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; Munuce, María J.; Vazquez, Monica Hebe; Marin Briggiler, Clara Isabel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Buffa, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Guillardoy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gongora, Adrian Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Munuce, María J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Sanatorio Británico de Rosario. Reprolab; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
SPERM
FIBROBLAST GROWTH FACTOR
FGFR
FGF
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10300
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10300
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulationSaucedo, LuciaBuffa, Gabriela NataliaRosso, MarinaGuillardoy, TomásGongora, Adrian DanielMunuce, María J.Vazquez, Monica HebeMarin Briggiler, Clara IsabelSPERMFIBROBLAST GROWTH FACTORFGFRFGFhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Buffa, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Guillardoy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Gongora, Adrian Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Munuce, María J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Sanatorio Británico de Rosario. Reprolab; ArgentinaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaPublic Library Of Science2015-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10300Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; et al.; Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation; Public Library Of Science; Plos One; 10; 5; 13-5-2015; e0127297-e01272971932-62031932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127297info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0127297info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430232/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:51Zoai:ri.conicet.gov.ar:11336/10300instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:52.27CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
title Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
spellingShingle Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
Saucedo, Lucia
SPERM
FIBROBLAST GROWTH FACTOR
FGFR
FGF
title_short Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
title_full Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
title_fullStr Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
title_full_unstemmed Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
title_sort Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
dc.creator.none.fl_str_mv Saucedo, Lucia
Buffa, Gabriela Natalia
Rosso, Marina
Guillardoy, Tomás
Gongora, Adrian Daniel
Munuce, María J.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author Saucedo, Lucia
author_facet Saucedo, Lucia
Buffa, Gabriela Natalia
Rosso, Marina
Guillardoy, Tomás
Gongora, Adrian Daniel
Munuce, María J.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author_role author
author2 Buffa, Gabriela Natalia
Rosso, Marina
Guillardoy, Tomás
Gongora, Adrian Daniel
Munuce, María J.
Vazquez, Monica Hebe
Marin Briggiler, Clara Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SPERM
FIBROBLAST GROWTH FACTOR
FGFR
FGF
topic SPERM
FIBROBLAST GROWTH FACTOR
FGFR
FGF
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Buffa, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Guillardoy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gongora, Adrian Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Munuce, María J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Sanatorio Británico de Rosario. Reprolab; Argentina
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10300
Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; et al.; Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation; Public Library Of Science; Plos One; 10; 5; 13-5-2015; e0127297-e0127297
1932-6203
1932-6203
url http://hdl.handle.net/11336/10300
identifier_str_mv Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; et al.; Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation; Public Library Of Science; Plos One; 10; 5; 13-5-2015; e0127297-e0127297
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127297
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0127297
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430232/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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