Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort

Autores
González, María Laura; Causada Calo, Natalia; Santino, Juan Pablo; Dominguez Valentin, Mev; Ferro, Fabiana Alejandra; Sammartino, Inés; Kalfayan, Pablo Germán; Verzura, Maria Alicia; Piñero, Tamara Lejandra; Cajal, Andrea; Pavicic, Walter Hernan; Vaccaro, Carlos
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microsatellite instability (MSI) is a hallmark tool for Lynch syndrome (LS) screening and a prognostic marker for sporadic colorectal cancer (CRC). In regions with limited resources and scarce CRC molecular characterization as South America, the implementation of universal MSI screening is under debate for both its purposes. We sought to estimate the frequency of BAT26 in colorectal adenocarcinomas and to determine associated clinical and histological features. Consecutive patients from a CRC registry were included. BAT26 determination was performed in all cases; if instability was found, immunohistochemistry (IHC) and BRAF mutation analyses were done, as appropriate. Differences were assessed by chi-squared or Fisher’s exact test, or by T test or Mann–Whitney. Multiple logistic regression was used to identify factors independently associated with BAT26-unstable tumors. We included 155 patients; mean age was 65.6 (SD 14.4) and 56.1% were male. The frequency of BAT26-unstable tumors was 22% (95% CI 15.7–29.3). Factors independently associated with BAT26-unstable tumors were right colon localization (OR 3.4, 95% CI 1.3–8.7), histological MSI features (OR 5.1, 95% CI 1.9–13.6) and Amsterdam criteria (OR 23.2, 95% CI 1.9–286.7). IHC was altered in 85.3% BAT26-unstable tumors and 70.6% lacked MLH1 expression; 47.8% of these harbored BRAF V600E mutation. We provide evidence to link the frequency of BAT26 to an increased diagnostic yield (up to 1.4-folds) of suspected LS cases in comparison to the revised Bethesda guidelines alone. In regions with limited resources, clinical and histological features associated with BAT26-unstable status could be useful to direct MSI screening in sporadic CRCs and may help guide clinical care and future research.
Fil: González, María Laura. Hospital Italiano; Argentina
Fil: Causada Calo, Natalia. Hospital Italiano; Argentina. McMaster University; Canadá
Fil: Santino, Juan Pablo. Hospital Italiano; Argentina
Fil: Dominguez Valentin, Mev. The Norwegian Radium Hospital; Noruega
Fil: Ferro, Fabiana Alejandra. Hospital Italiano; Argentina
Fil: Sammartino, Inés. Hospital Italiano; Argentina
Fil: Kalfayan, Pablo Germán. Hospital Italiano; Argentina
Fil: Verzura, Maria Alicia. Hospital Italiano; Argentina
Fil: Piñero, Tamara Lejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Cajal, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Pavicic, Walter Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Vaccaro, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Materia
Bat26
Colorectal Cancer
Lynch Syndrome
Microsatellite Instability
Universal Screening
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66087
Acceder
id CONICETDig_782c4c5d122d47a1bd6d3331ad5bc999
oai_identifier_str oai:ri.conicet.gov.ar:11336/66087
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohortGonzález, María LauraCausada Calo, NataliaSantino, Juan PabloDominguez Valentin, MevFerro, Fabiana AlejandraSammartino, InésKalfayan, Pablo GermánVerzura, Maria AliciaPiñero, Tamara LejandraCajal, AndreaPavicic, Walter HernanVaccaro, CarlosBat26Colorectal CancerLynch SyndromeMicrosatellite InstabilityUniversal Screeninghttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Microsatellite instability (MSI) is a hallmark tool for Lynch syndrome (LS) screening and a prognostic marker for sporadic colorectal cancer (CRC). In regions with limited resources and scarce CRC molecular characterization as South America, the implementation of universal MSI screening is under debate for both its purposes. We sought to estimate the frequency of BAT26 in colorectal adenocarcinomas and to determine associated clinical and histological features. Consecutive patients from a CRC registry were included. BAT26 determination was performed in all cases; if instability was found, immunohistochemistry (IHC) and BRAF mutation analyses were done, as appropriate. Differences were assessed by chi-squared or Fisher’s exact test, or by T test or Mann–Whitney. Multiple logistic regression was used to identify factors independently associated with BAT26-unstable tumors. We included 155 patients; mean age was 65.6 (SD 14.4) and 56.1% were male. The frequency of BAT26-unstable tumors was 22% (95% CI 15.7–29.3). Factors independently associated with BAT26-unstable tumors were right colon localization (OR 3.4, 95% CI 1.3–8.7), histological MSI features (OR 5.1, 95% CI 1.9–13.6) and Amsterdam criteria (OR 23.2, 95% CI 1.9–286.7). IHC was altered in 85.3% BAT26-unstable tumors and 70.6% lacked MLH1 expression; 47.8% of these harbored BRAF V600E mutation. We provide evidence to link the frequency of BAT26 to an increased diagnostic yield (up to 1.4-folds) of suspected LS cases in comparison to the revised Bethesda guidelines alone. In regions with limited resources, clinical and histological features associated with BAT26-unstable status could be useful to direct MSI screening in sporadic CRCs and may help guide clinical care and future research.Fil: González, María Laura. Hospital Italiano; ArgentinaFil: Causada Calo, Natalia. Hospital Italiano; Argentina. McMaster University; CanadáFil: Santino, Juan Pablo. Hospital Italiano; ArgentinaFil: Dominguez Valentin, Mev. The Norwegian Radium Hospital; NoruegaFil: Ferro, Fabiana Alejandra. Hospital Italiano; ArgentinaFil: Sammartino, Inés. Hospital Italiano; ArgentinaFil: Kalfayan, Pablo Germán. Hospital Italiano; ArgentinaFil: Verzura, Maria Alicia. Hospital Italiano; ArgentinaFil: Piñero, Tamara Lejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Cajal, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Pavicic, Walter Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Vaccaro, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaSpringer2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66087González, María Laura; Causada Calo, Natalia; Santino, Juan Pablo; Dominguez Valentin, Mev; Ferro, Fabiana Alejandra; et al.; Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort; Springer; Familial Cancer; 17; 3; 7-2018; 395-4021389-9600CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10689-017-0052-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s10689-017-0052-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:31Zoai:ri.conicet.gov.ar:11336/66087instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:31.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
title Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
spellingShingle Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
González, María Laura
Bat26
Colorectal Cancer
Lynch Syndrome
Microsatellite Instability
Universal Screening
title_short Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
title_full Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
title_fullStr Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
title_full_unstemmed Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
title_sort Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort
dc.creator.none.fl_str_mv González, María Laura
Causada Calo, Natalia
Santino, Juan Pablo
Dominguez Valentin, Mev
Ferro, Fabiana Alejandra
Sammartino, Inés
Kalfayan, Pablo Germán
Verzura, Maria Alicia
Piñero, Tamara Lejandra
Cajal, Andrea
Pavicic, Walter Hernan
Vaccaro, Carlos
author González, María Laura
author_facet González, María Laura
Causada Calo, Natalia
Santino, Juan Pablo
Dominguez Valentin, Mev
Ferro, Fabiana Alejandra
Sammartino, Inés
Kalfayan, Pablo Germán
Verzura, Maria Alicia
Piñero, Tamara Lejandra
Cajal, Andrea
Pavicic, Walter Hernan
Vaccaro, Carlos
author_role author
author2 Causada Calo, Natalia
Santino, Juan Pablo
Dominguez Valentin, Mev
Ferro, Fabiana Alejandra
Sammartino, Inés
Kalfayan, Pablo Germán
Verzura, Maria Alicia
Piñero, Tamara Lejandra
Cajal, Andrea
Pavicic, Walter Hernan
Vaccaro, Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bat26
Colorectal Cancer
Lynch Syndrome
Microsatellite Instability
Universal Screening
topic Bat26
Colorectal Cancer
Lynch Syndrome
Microsatellite Instability
Universal Screening
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Microsatellite instability (MSI) is a hallmark tool for Lynch syndrome (LS) screening and a prognostic marker for sporadic colorectal cancer (CRC). In regions with limited resources and scarce CRC molecular characterization as South America, the implementation of universal MSI screening is under debate for both its purposes. We sought to estimate the frequency of BAT26 in colorectal adenocarcinomas and to determine associated clinical and histological features. Consecutive patients from a CRC registry were included. BAT26 determination was performed in all cases; if instability was found, immunohistochemistry (IHC) and BRAF mutation analyses were done, as appropriate. Differences were assessed by chi-squared or Fisher’s exact test, or by T test or Mann–Whitney. Multiple logistic regression was used to identify factors independently associated with BAT26-unstable tumors. We included 155 patients; mean age was 65.6 (SD 14.4) and 56.1% were male. The frequency of BAT26-unstable tumors was 22% (95% CI 15.7–29.3). Factors independently associated with BAT26-unstable tumors were right colon localization (OR 3.4, 95% CI 1.3–8.7), histological MSI features (OR 5.1, 95% CI 1.9–13.6) and Amsterdam criteria (OR 23.2, 95% CI 1.9–286.7). IHC was altered in 85.3% BAT26-unstable tumors and 70.6% lacked MLH1 expression; 47.8% of these harbored BRAF V600E mutation. We provide evidence to link the frequency of BAT26 to an increased diagnostic yield (up to 1.4-folds) of suspected LS cases in comparison to the revised Bethesda guidelines alone. In regions with limited resources, clinical and histological features associated with BAT26-unstable status could be useful to direct MSI screening in sporadic CRCs and may help guide clinical care and future research.
Fil: González, María Laura. Hospital Italiano; Argentina
Fil: Causada Calo, Natalia. Hospital Italiano; Argentina. McMaster University; Canadá
Fil: Santino, Juan Pablo. Hospital Italiano; Argentina
Fil: Dominguez Valentin, Mev. The Norwegian Radium Hospital; Noruega
Fil: Ferro, Fabiana Alejandra. Hospital Italiano; Argentina
Fil: Sammartino, Inés. Hospital Italiano; Argentina
Fil: Kalfayan, Pablo Germán. Hospital Italiano; Argentina
Fil: Verzura, Maria Alicia. Hospital Italiano; Argentina
Fil: Piñero, Tamara Lejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Cajal, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Pavicic, Walter Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Vaccaro, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
description Microsatellite instability (MSI) is a hallmark tool for Lynch syndrome (LS) screening and a prognostic marker for sporadic colorectal cancer (CRC). In regions with limited resources and scarce CRC molecular characterization as South America, the implementation of universal MSI screening is under debate for both its purposes. We sought to estimate the frequency of BAT26 in colorectal adenocarcinomas and to determine associated clinical and histological features. Consecutive patients from a CRC registry were included. BAT26 determination was performed in all cases; if instability was found, immunohistochemistry (IHC) and BRAF mutation analyses were done, as appropriate. Differences were assessed by chi-squared or Fisher’s exact test, or by T test or Mann–Whitney. Multiple logistic regression was used to identify factors independently associated with BAT26-unstable tumors. We included 155 patients; mean age was 65.6 (SD 14.4) and 56.1% were male. The frequency of BAT26-unstable tumors was 22% (95% CI 15.7–29.3). Factors independently associated with BAT26-unstable tumors were right colon localization (OR 3.4, 95% CI 1.3–8.7), histological MSI features (OR 5.1, 95% CI 1.9–13.6) and Amsterdam criteria (OR 23.2, 95% CI 1.9–286.7). IHC was altered in 85.3% BAT26-unstable tumors and 70.6% lacked MLH1 expression; 47.8% of these harbored BRAF V600E mutation. We provide evidence to link the frequency of BAT26 to an increased diagnostic yield (up to 1.4-folds) of suspected LS cases in comparison to the revised Bethesda guidelines alone. In regions with limited resources, clinical and histological features associated with BAT26-unstable status could be useful to direct MSI screening in sporadic CRCs and may help guide clinical care and future research.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66087
González, María Laura; Causada Calo, Natalia; Santino, Juan Pablo; Dominguez Valentin, Mev; Ferro, Fabiana Alejandra; et al.; Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort; Springer; Familial Cancer; 17; 3; 7-2018; 395-402
1389-9600
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66087
identifier_str_mv González, María Laura; Causada Calo, Natalia; Santino, Juan Pablo; Dominguez Valentin, Mev; Ferro, Fabiana Alejandra; et al.; Universal determination of microsatellite instability using BAT26 as a single marker in an Argentine colorectal cancer cohort; Springer; Familial Cancer; 17; 3; 7-2018; 395-402
1389-9600
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10689-017-0052-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10689-017-0052-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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