CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR

Autores
Sánchez, A.G.; Juaneda, I.; Eynard, H.; Basquiera, Ana Lisa; Palazzo, E.; Calafat, P.; Palla, V.; Romagnoli, Pablo Alberto; Alvarellos, T.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples. Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in c‐kit) and BAT26 (a poly(A) tract localized in hMSH2) microsatellites were assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with jigj MSI. Allelic size variation of CAT25 was analysed on peripheral blood DNA from 208 healthy volunteers. Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64–66 base pairs. Of 110 tumours, 11 had High MSI, later confirmed by IHC. CAT25 defines MSI alone as well as when used together with BAT25 and BAT26. CAT25 results provided 100% predictive values and p < 0.0001 to classify a tumour as having high MSI. Conclusions: We developed and validated a new HRM-PCR assay to detect CAT25 instability. Our findings showed a limited allelic size variation of CAT25 and highlighted to CAT25 as a promising marker for MSI analysis.
Fil: Sánchez, A.G.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
Fil: Juaneda, I.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Eynard, H.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Basquiera, Ana Lisa. Hospital Privado Universitario de Córdoba; Argentina
Fil: Palazzo, E.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Calafat, P.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Palla, V.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Romagnoli, Pablo Alberto. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Instituto de Investigación Médica Mercedes y Martín Ferreyra. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa; Argentina
Fil: Alvarellos, T.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
Materia
CAT25-MARKER
COLORECTAL CANCER
HIGH-RESOLUTION MELTING
MICROSATELLITE INSTABILITY
REAL-TIME PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140273
Acceder
id CONICETDig_34d19613f0adce8edc25ab948d396511
oai_identifier_str oai:ri.conicet.gov.ar:11336/140273
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCRSánchez, A.G.Juaneda, I.Eynard, H.Basquiera, Ana LisaPalazzo, E.Calafat, P.Palla, V.Romagnoli, Pablo AlbertoAlvarellos, T.CAT25-MARKERCOLORECTAL CANCERHIGH-RESOLUTION MELTINGMICROSATELLITE INSTABILITYREAL-TIME PCRhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples. Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in c‐kit) and BAT26 (a poly(A) tract localized in hMSH2) microsatellites were assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with jigj MSI. Allelic size variation of CAT25 was analysed on peripheral blood DNA from 208 healthy volunteers. Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64–66 base pairs. Of 110 tumours, 11 had High MSI, later confirmed by IHC. CAT25 defines MSI alone as well as when used together with BAT25 and BAT26. CAT25 results provided 100% predictive values and p < 0.0001 to classify a tumour as having high MSI. Conclusions: We developed and validated a new HRM-PCR assay to detect CAT25 instability. Our findings showed a limited allelic size variation of CAT25 and highlighted to CAT25 as a promising marker for MSI analysis.Fil: Sánchez, A.G.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; ArgentinaFil: Juaneda, I.. Hospital Privado Universitario de Córdoba; ArgentinaFil: Eynard, H.. Hospital Privado Universitario de Córdoba; ArgentinaFil: Basquiera, Ana Lisa. Hospital Privado Universitario de Córdoba; ArgentinaFil: Palazzo, E.. Hospital Privado Universitario de Córdoba; ArgentinaFil: Calafat, P.. Hospital Privado Universitario de Córdoba; ArgentinaFil: Palla, V.. Hospital Privado Universitario de Córdoba; ArgentinaFil: Romagnoli, Pablo Alberto. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Instituto de Investigación Médica Mercedes y Martín Ferreyra. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa; ArgentinaFil: Alvarellos, T.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; ArgentinaStep Publishing Ltd2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140273Sánchez, A.G.; Juaneda, I.; Eynard, H.; Basquiera, Ana Lisa; Palazzo, E.; et al.; CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR; Step Publishing Ltd; British Journal Of Biomedical Science; 77; 3; 7-2020; 105-1110967-4845CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09674845.2020.1730625info:eu-repo/semantics/altIdentifier/doi/10.1080/09674845.2020.1730625info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:04Zoai:ri.conicet.gov.ar:11336/140273instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:04.842CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
title CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
spellingShingle CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
Sánchez, A.G.
CAT25-MARKER
COLORECTAL CANCER
HIGH-RESOLUTION MELTING
MICROSATELLITE INSTABILITY
REAL-TIME PCR
title_short CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
title_full CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
title_fullStr CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
title_full_unstemmed CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
title_sort CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR
dc.creator.none.fl_str_mv Sánchez, A.G.
Juaneda, I.
Eynard, H.
Basquiera, Ana Lisa
Palazzo, E.
Calafat, P.
Palla, V.
Romagnoli, Pablo Alberto
Alvarellos, T.
author Sánchez, A.G.
author_facet Sánchez, A.G.
Juaneda, I.
Eynard, H.
Basquiera, Ana Lisa
Palazzo, E.
Calafat, P.
Palla, V.
Romagnoli, Pablo Alberto
Alvarellos, T.
author_role author
author2 Juaneda, I.
Eynard, H.
Basquiera, Ana Lisa
Palazzo, E.
Calafat, P.
Palla, V.
Romagnoli, Pablo Alberto
Alvarellos, T.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAT25-MARKER
COLORECTAL CANCER
HIGH-RESOLUTION MELTING
MICROSATELLITE INSTABILITY
REAL-TIME PCR
topic CAT25-MARKER
COLORECTAL CANCER
HIGH-RESOLUTION MELTING
MICROSATELLITE INSTABILITY
REAL-TIME PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples. Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in c‐kit) and BAT26 (a poly(A) tract localized in hMSH2) microsatellites were assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with jigj MSI. Allelic size variation of CAT25 was analysed on peripheral blood DNA from 208 healthy volunteers. Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64–66 base pairs. Of 110 tumours, 11 had High MSI, later confirmed by IHC. CAT25 defines MSI alone as well as when used together with BAT25 and BAT26. CAT25 results provided 100% predictive values and p < 0.0001 to classify a tumour as having high MSI. Conclusions: We developed and validated a new HRM-PCR assay to detect CAT25 instability. Our findings showed a limited allelic size variation of CAT25 and highlighted to CAT25 as a promising marker for MSI analysis.
Fil: Sánchez, A.G.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
Fil: Juaneda, I.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Eynard, H.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Basquiera, Ana Lisa. Hospital Privado Universitario de Córdoba; Argentina
Fil: Palazzo, E.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Calafat, P.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Palla, V.. Hospital Privado Universitario de Córdoba; Argentina
Fil: Romagnoli, Pablo Alberto. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Instituto de Investigación Médica Mercedes y Martín Ferreyra. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa; Argentina
Fil: Alvarellos, T.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
description Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples. Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in c‐kit) and BAT26 (a poly(A) tract localized in hMSH2) microsatellites were assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with jigj MSI. Allelic size variation of CAT25 was analysed on peripheral blood DNA from 208 healthy volunteers. Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64–66 base pairs. Of 110 tumours, 11 had High MSI, later confirmed by IHC. CAT25 defines MSI alone as well as when used together with BAT25 and BAT26. CAT25 results provided 100% predictive values and p < 0.0001 to classify a tumour as having high MSI. Conclusions: We developed and validated a new HRM-PCR assay to detect CAT25 instability. Our findings showed a limited allelic size variation of CAT25 and highlighted to CAT25 as a promising marker for MSI analysis.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140273
Sánchez, A.G.; Juaneda, I.; Eynard, H.; Basquiera, Ana Lisa; Palazzo, E.; et al.; CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR; Step Publishing Ltd; British Journal Of Biomedical Science; 77; 3; 7-2020; 105-111
0967-4845
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140273
identifier_str_mv Sánchez, A.G.; Juaneda, I.; Eynard, H.; Basquiera, Ana Lisa; Palazzo, E.; et al.; CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR; Step Publishing Ltd; British Journal Of Biomedical Science; 77; 3; 7-2020; 105-111
0967-4845
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09674845.2020.1730625
info:eu-repo/semantics/altIdentifier/doi/10.1080/09674845.2020.1730625
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Step Publishing Ltd
publisher.none.fl_str_mv Step Publishing Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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