Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease

Autores
Bondar, Constanza María; Araya, Romina Elizabeth; Guzman, Luciana; Cueto Rua, Eduardo; Chopita, Néstor; Chirdo, Fernando Gabriel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lymphocytic infiltration in the lamina propria (LP), which is primarily composed of CD4+ Th1 cells and plasma cells, and increased numbers of intraepithelial lymphocytes (IELs), is a characteristic finding in active Celiac Disease (CD). Signals for this selective cell recruitment have not been fully established. CXCR3 and its ligands, particularly CXCL10, were suggested to be one of the most relevant pathways in attracting cells into inflamed tissues. In addition, CXCR3 is characteristically expressed by Th1 cells. The aim of this work was to investigate the participation of the chemokine CXCL10/CXCR3 axis in CD pathogenesis. A higher concentration of CXCL10 was found in the serum from untreated CD patients. mRNA levels for CXCL10 and CXCL11 but not CXCL9 were significantly higher in duodenal biopsies from untreated CD patients compared with non-CD controls or treated patients. CXCL10 was observed to be abundantly produced in untreated CD; the expression of CXCL10 correlated with IFN levels in the tissue and was reduced in treated patients. Plasma cells and enterocytes were identified as CXCL10 producer cells. Moreover, CXCL10 expression in intestinal tissues was upregulated by poly I:C or IL-15. IELs, LP T lymphocytes, and plasma cells, which infiltrate major populations in intestinal mucosa in untreated CD, express CXCR3. The CXCR3/CXCL10 signalling axis is overactivated in the intestinal mucosa in untreated patients, which explains the specific recruitment of the major cell populations that infiltrate the epithelium, as well as the LP, in CD.
Fil: Bondar, Constanza María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Araya, Romina Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Guzman, Luciana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; Argentina
Fil: Cueto Rua, Eduardo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; Argentina
Fil: Chopita, Néstor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Materia
celiac disease
chemokines
inflammation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31252
Acceder
id CONICETDig_77cd60ea08d4912ac28d2d41aadca3b7
oai_identifier_str oai:ri.conicet.gov.ar:11336/31252
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac DiseaseBondar, Constanza MaríaAraya, Romina ElizabethGuzman, LucianaCueto Rua, EduardoChopita, NéstorChirdo, Fernando Gabrielceliac diseasechemokinesinflammationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lymphocytic infiltration in the lamina propria (LP), which is primarily composed of CD4+ Th1 cells and plasma cells, and increased numbers of intraepithelial lymphocytes (IELs), is a characteristic finding in active Celiac Disease (CD). Signals for this selective cell recruitment have not been fully established. CXCR3 and its ligands, particularly CXCL10, were suggested to be one of the most relevant pathways in attracting cells into inflamed tissues. In addition, CXCR3 is characteristically expressed by Th1 cells. The aim of this work was to investigate the participation of the chemokine CXCL10/CXCR3 axis in CD pathogenesis. A higher concentration of CXCL10 was found in the serum from untreated CD patients. mRNA levels for CXCL10 and CXCL11 but not CXCL9 were significantly higher in duodenal biopsies from untreated CD patients compared with non-CD controls or treated patients. CXCL10 was observed to be abundantly produced in untreated CD; the expression of CXCL10 correlated with IFN levels in the tissue and was reduced in treated patients. Plasma cells and enterocytes were identified as CXCL10 producer cells. Moreover, CXCL10 expression in intestinal tissues was upregulated by poly I:C or IL-15. IELs, LP T lymphocytes, and plasma cells, which infiltrate major populations in intestinal mucosa in untreated CD, express CXCR3. The CXCR3/CXCL10 signalling axis is overactivated in the intestinal mucosa in untreated patients, which explains the specific recruitment of the major cell populations that infiltrate the epithelium, as well as the LP, in CD.Fil: Bondar, Constanza María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Araya, Romina Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Guzman, Luciana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; ArgentinaFil: Cueto Rua, Eduardo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; ArgentinaFil: Chopita, Néstor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; ArgentinaFil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; ArgentinaPublic Library of Science2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31252Chirdo, Fernando Gabriel; Chopita, Néstor; Cueto Rua, Eduardo; Guzman, Luciana; Araya, Romina Elizabeth; Bondar, Constanza María; et al.; Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease; Public Library of Science; Plos One; 9; 2; 2-2014; 1-12; e890681932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0089068info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:58Zoai:ri.conicet.gov.ar:11336/31252instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:58.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
title Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
spellingShingle Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
Bondar, Constanza María
celiac disease
chemokines
inflammation
title_short Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
title_full Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
title_fullStr Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
title_full_unstemmed Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
title_sort Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease
dc.creator.none.fl_str_mv Bondar, Constanza María
Araya, Romina Elizabeth
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor
Chirdo, Fernando Gabriel
author Bondar, Constanza María
author_facet Bondar, Constanza María
Araya, Romina Elizabeth
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor
Chirdo, Fernando Gabriel
author_role author
author2 Araya, Romina Elizabeth
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor
Chirdo, Fernando Gabriel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv celiac disease
chemokines
inflammation
topic celiac disease
chemokines
inflammation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lymphocytic infiltration in the lamina propria (LP), which is primarily composed of CD4+ Th1 cells and plasma cells, and increased numbers of intraepithelial lymphocytes (IELs), is a characteristic finding in active Celiac Disease (CD). Signals for this selective cell recruitment have not been fully established. CXCR3 and its ligands, particularly CXCL10, were suggested to be one of the most relevant pathways in attracting cells into inflamed tissues. In addition, CXCR3 is characteristically expressed by Th1 cells. The aim of this work was to investigate the participation of the chemokine CXCL10/CXCR3 axis in CD pathogenesis. A higher concentration of CXCL10 was found in the serum from untreated CD patients. mRNA levels for CXCL10 and CXCL11 but not CXCL9 were significantly higher in duodenal biopsies from untreated CD patients compared with non-CD controls or treated patients. CXCL10 was observed to be abundantly produced in untreated CD; the expression of CXCL10 correlated with IFN levels in the tissue and was reduced in treated patients. Plasma cells and enterocytes were identified as CXCL10 producer cells. Moreover, CXCL10 expression in intestinal tissues was upregulated by poly I:C or IL-15. IELs, LP T lymphocytes, and plasma cells, which infiltrate major populations in intestinal mucosa in untreated CD, express CXCR3. The CXCR3/CXCL10 signalling axis is overactivated in the intestinal mucosa in untreated patients, which explains the specific recruitment of the major cell populations that infiltrate the epithelium, as well as the LP, in CD.
Fil: Bondar, Constanza María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Araya, Romina Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Guzman, Luciana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; Argentina
Fil: Cueto Rua, Eduardo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños ; Argentina
Fil: Chopita, Néstor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Chirdo, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
description Lymphocytic infiltration in the lamina propria (LP), which is primarily composed of CD4+ Th1 cells and plasma cells, and increased numbers of intraepithelial lymphocytes (IELs), is a characteristic finding in active Celiac Disease (CD). Signals for this selective cell recruitment have not been fully established. CXCR3 and its ligands, particularly CXCL10, were suggested to be one of the most relevant pathways in attracting cells into inflamed tissues. In addition, CXCR3 is characteristically expressed by Th1 cells. The aim of this work was to investigate the participation of the chemokine CXCL10/CXCR3 axis in CD pathogenesis. A higher concentration of CXCL10 was found in the serum from untreated CD patients. mRNA levels for CXCL10 and CXCL11 but not CXCL9 were significantly higher in duodenal biopsies from untreated CD patients compared with non-CD controls or treated patients. CXCL10 was observed to be abundantly produced in untreated CD; the expression of CXCL10 correlated with IFN levels in the tissue and was reduced in treated patients. Plasma cells and enterocytes were identified as CXCL10 producer cells. Moreover, CXCL10 expression in intestinal tissues was upregulated by poly I:C or IL-15. IELs, LP T lymphocytes, and plasma cells, which infiltrate major populations in intestinal mucosa in untreated CD, express CXCR3. The CXCR3/CXCL10 signalling axis is overactivated in the intestinal mucosa in untreated patients, which explains the specific recruitment of the major cell populations that infiltrate the epithelium, as well as the LP, in CD.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31252
Chirdo, Fernando Gabriel; Chopita, Néstor; Cueto Rua, Eduardo; Guzman, Luciana; Araya, Romina Elizabeth; Bondar, Constanza María; et al.; Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease; Public Library of Science; Plos One; 9; 2; 2-2014; 1-12; e89068
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31252
identifier_str_mv Chirdo, Fernando Gabriel; Chopita, Néstor; Cueto Rua, Eduardo; Guzman, Luciana; Araya, Romina Elizabeth; Bondar, Constanza María; et al.; Role of CXCR3/CXCL10 Axis in Immune Cell Recruitment into the Small Intestine in Celiac Disease; Public Library of Science; Plos One; 9; 2; 2-2014; 1-12; e89068
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0089068
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980302209679360
score 12.993085

Publicaciones similares