Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis

Autores
Martin, Anandi; Imperiale, Belén Rocío; Ravolonandriana, Pascaline; Coban, Ahmet Yilmaz; Akgunes, Alper; Ikram, Aamer; Satti, Luqman; Odoun, Mathieu; Pandey, Pooja; Mishra, Manvi; Affolabi, Dissou; Singh, Urvashi; Rasolofo, Voahangy; Morcillo, Nora Susana; Vandamme, Peter; Palomino, Juan Carlos
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.
Fil: Martin, Anandi. University of Ghent; Bélgica
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Ravolonandriana, Pascaline. Institut Pasteur de Madagascar; Madagascar
Fil: Coban, Ahmet Yilmaz. Ondokuz Mayis University; Turquía
Fil: Akgunes, Alper. Chest Diseases Hospital; Turquía
Fil: Ikram, Aamer. Combined Military Hospital; Pakistán
Fil: Satti, Luqman. Combined Military Hospital; Pakistán
Fil: Odoun, Mathieu. Laboratoire de Reference des Mycobacterie; Benín
Fil: Pandey, Pooja. All India Institute of Medical Sciences; India
Fil: Mishra, Manvi. All India Institute of Medical Sciences; India
Fil: Affolabi, Dissou. Laboratoire de Reference des Mycobacterie; Benín
Fil: Singh, Urvashi. All India Institute of Medical Sciences; India
Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Vandamme, Peter. University of Ghent; Bélgica
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
Materia
MULTIDRUG RESISTANCE
MYCOBACTERIUM TUBERCULOSIS
NITRATASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88817
Acceder
id CONICETDig_d40ebef74d91d3296a09002ddcd9519f
oai_identifier_str oai:ri.conicet.gov.ar:11336/88817
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosisMartin, AnandiImperiale, Belén RocíoRavolonandriana, PascalineCoban, Ahmet YilmazAkgunes, AlperIkram, AamerSatti, LuqmanOdoun, MathieuPandey, PoojaMishra, ManviAffolabi, DissouSingh, UrvashiRasolofo, VoahangyMorcillo, Nora SusanaVandamme, PeterPalomino, Juan CarlosMULTIDRUG RESISTANCEMYCOBACTERIUM TUBERCULOSISNITRATASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.Fil: Martin, Anandi. University of Ghent; BélgicaFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Ravolonandriana, Pascaline. Institut Pasteur de Madagascar; MadagascarFil: Coban, Ahmet Yilmaz. Ondokuz Mayis University; TurquíaFil: Akgunes, Alper. Chest Diseases Hospital; TurquíaFil: Ikram, Aamer. Combined Military Hospital; PakistánFil: Satti, Luqman. Combined Military Hospital; PakistánFil: Odoun, Mathieu. Laboratoire de Reference des Mycobacterie; BenínFil: Pandey, Pooja. All India Institute of Medical Sciences; IndiaFil: Mishra, Manvi. All India Institute of Medical Sciences; IndiaFil: Affolabi, Dissou. Laboratoire de Reference des Mycobacterie; BenínFil: Singh, Urvashi. All India Institute of Medical Sciences; IndiaFil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; MadagascarFil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Vandamme, Peter. University of Ghent; BélgicaFil: Palomino, Juan Carlos. University of Ghent; BélgicaOxford University Press2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88817Martin, Anandi; Imperiale, Belén Rocío; Ravolonandriana, Pascaline; Coban, Ahmet Yilmaz; Akgunes, Alper; et al.; Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 69; 2; 2-2014; 441-4440305-7453CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkt353info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/69/2/441/711920info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:22Zoai:ri.conicet.gov.ar:11336/88817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:22.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
title Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
spellingShingle Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
Martin, Anandi
MULTIDRUG RESISTANCE
MYCOBACTERIUM TUBERCULOSIS
NITRATASE
title_short Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
title_full Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
title_fullStr Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
title_full_unstemmed Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
title_sort Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
dc.creator.none.fl_str_mv Martin, Anandi
Imperiale, Belén Rocío
Ravolonandriana, Pascaline
Coban, Ahmet Yilmaz
Akgunes, Alper
Ikram, Aamer
Satti, Luqman
Odoun, Mathieu
Pandey, Pooja
Mishra, Manvi
Affolabi, Dissou
Singh, Urvashi
Rasolofo, Voahangy
Morcillo, Nora Susana
Vandamme, Peter
Palomino, Juan Carlos
author Martin, Anandi
author_facet Martin, Anandi
Imperiale, Belén Rocío
Ravolonandriana, Pascaline
Coban, Ahmet Yilmaz
Akgunes, Alper
Ikram, Aamer
Satti, Luqman
Odoun, Mathieu
Pandey, Pooja
Mishra, Manvi
Affolabi, Dissou
Singh, Urvashi
Rasolofo, Voahangy
Morcillo, Nora Susana
Vandamme, Peter
Palomino, Juan Carlos
author_role author
author2 Imperiale, Belén Rocío
Ravolonandriana, Pascaline
Coban, Ahmet Yilmaz
Akgunes, Alper
Ikram, Aamer
Satti, Luqman
Odoun, Mathieu
Pandey, Pooja
Mishra, Manvi
Affolabi, Dissou
Singh, Urvashi
Rasolofo, Voahangy
Morcillo, Nora Susana
Vandamme, Peter
Palomino, Juan Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MULTIDRUG RESISTANCE
MYCOBACTERIUM TUBERCULOSIS
NITRATASE
topic MULTIDRUG RESISTANCE
MYCOBACTERIUM TUBERCULOSIS
NITRATASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.
Fil: Martin, Anandi. University of Ghent; Bélgica
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Ravolonandriana, Pascaline. Institut Pasteur de Madagascar; Madagascar
Fil: Coban, Ahmet Yilmaz. Ondokuz Mayis University; Turquía
Fil: Akgunes, Alper. Chest Diseases Hospital; Turquía
Fil: Ikram, Aamer. Combined Military Hospital; Pakistán
Fil: Satti, Luqman. Combined Military Hospital; Pakistán
Fil: Odoun, Mathieu. Laboratoire de Reference des Mycobacterie; Benín
Fil: Pandey, Pooja. All India Institute of Medical Sciences; India
Fil: Mishra, Manvi. All India Institute of Medical Sciences; India
Fil: Affolabi, Dissou. Laboratoire de Reference des Mycobacterie; Benín
Fil: Singh, Urvashi. All India Institute of Medical Sciences; India
Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Vandamme, Peter. University of Ghent; Bélgica
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
description Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88817
Martin, Anandi; Imperiale, Belén Rocío; Ravolonandriana, Pascaline; Coban, Ahmet Yilmaz; Akgunes, Alper; et al.; Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 69; 2; 2-2014; 441-444
0305-7453
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88817
identifier_str_mv Martin, Anandi; Imperiale, Belén Rocío; Ravolonandriana, Pascaline; Coban, Ahmet Yilmaz; Akgunes, Alper; et al.; Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 69; 2; 2-2014; 441-444
0305-7453
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkt353
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/69/2/441/711920
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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