PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia

Autores
Macias, Ana Maria; Romero, Nuria Magdalena; Martín, Francisco; Suárez, Leonardo; Rosa, Alberto Luis; Morata, Ginés
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Drosophila adult male terminalia originate from the genital disc. During the pupal stages, the external parts of terminalia evert from two ventral stalks; the everted left and right dorsal halves fuse at the dorsal midline. At the same time the male terminalia perform a 360 clockwise rotation. Several mutations are known to affect the rotation of the male terminalia, while none is known to affect dorsal closure. We show here that the Pvf1 gene, encoding one of the three Drosophila homologues of the mammalian VEGF/PDGF growth factors, is required for both processes. Males either mutant for Pvf1 or bearing a dominant negative form of Pvr or stasis (stai), the unique PVF receptor, do not complete either rotation or dorsal closure. Pvf1 expression in the genital disc is restricted to the A8 cells. However, PVF1/PVR signaling influences A8, A9 and A10 cells, suggesting that the PVF1 protein diffuses from its source. Flies hemizygous for the apoptotic genes hid, reaper and grim, or mutant for puckered which encodes a phosphatase that down-regulates the n-Jun-N terminal kinase pathway, lead to the same phenotypes as mutations in PVF1/PVR. Our results indicate that PVF1/PVR signaling functions not only in apoptotic phenomena but are also required during rotation and dorsal closure of the Drosophila male genital disc.
Fil: Macias, Ana Maria. Universidad Nacional de Córdoba; Argentina
Fil: Romero, Nuria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Martín, Francisco. Consejo Superior de Investigaciones Científicas; España
Fil: Suárez, Leonardo. Universidad Nacional de Córdoba; Argentina
Fil: Rosa, Alberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Morata, Ginés. Consejo Superior de Investigaciones Científicas; España
Materia
APOPTOSIS
DROSOPHILA
MALE GENITAL DISC
PVF1/PVR
VEGF/PDGF
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96213
Acceder
id CONICETDig_76f299479d93c5c7b0181139a0658337
oai_identifier_str oai:ri.conicet.gov.ar:11336/96213
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminaliaMacias, Ana MariaRomero, Nuria MagdalenaMartín, FranciscoSuárez, LeonardoRosa, Alberto LuisMorata, GinésAPOPTOSISDROSOPHILAMALE GENITAL DISCPVF1/PVRVEGF/PDGFhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Drosophila adult male terminalia originate from the genital disc. During the pupal stages, the external parts of terminalia evert from two ventral stalks; the everted left and right dorsal halves fuse at the dorsal midline. At the same time the male terminalia perform a 360 clockwise rotation. Several mutations are known to affect the rotation of the male terminalia, while none is known to affect dorsal closure. We show here that the Pvf1 gene, encoding one of the three Drosophila homologues of the mammalian VEGF/PDGF growth factors, is required for both processes. Males either mutant for Pvf1 or bearing a dominant negative form of Pvr or stasis (stai), the unique PVF receptor, do not complete either rotation or dorsal closure. Pvf1 expression in the genital disc is restricted to the A8 cells. However, PVF1/PVR signaling influences A8, A9 and A10 cells, suggesting that the PVF1 protein diffuses from its source. Flies hemizygous for the apoptotic genes hid, reaper and grim, or mutant for puckered which encodes a phosphatase that down-regulates the n-Jun-N terminal kinase pathway, lead to the same phenotypes as mutations in PVF1/PVR. Our results indicate that PVF1/PVR signaling functions not only in apoptotic phenomena but are also required during rotation and dorsal closure of the Drosophila male genital disc.Fil: Macias, Ana Maria. Universidad Nacional de Córdoba; ArgentinaFil: Romero, Nuria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Martín, Francisco. Consejo Superior de Investigaciones Científicas; EspañaFil: Suárez, Leonardo. Universidad Nacional de Córdoba; ArgentinaFil: Rosa, Alberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Morata, Ginés. Consejo Superior de Investigaciones Científicas; EspañaU B C Press2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96213Macias, Ana Maria; Romero, Nuria Magdalena; Martín, Francisco; Suárez, Leonardo; Rosa, Alberto Luis; et al.; PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia; U B C Press; International Journal Of Developmental Biology; 48; 10; 12-2004; 1087-10940214-62821696-3547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.041859aminfo:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=10.1387/ijdb.041859aminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:11:40Zoai:ri.conicet.gov.ar:11336/96213instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:11:40.547CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
title PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
spellingShingle PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
Macias, Ana Maria
APOPTOSIS
DROSOPHILA
MALE GENITAL DISC
PVF1/PVR
VEGF/PDGF
title_short PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
title_full PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
title_fullStr PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
title_full_unstemmed PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
title_sort PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia
dc.creator.none.fl_str_mv Macias, Ana Maria
Romero, Nuria Magdalena
Martín, Francisco
Suárez, Leonardo
Rosa, Alberto Luis
Morata, Ginés
author Macias, Ana Maria
author_facet Macias, Ana Maria
Romero, Nuria Magdalena
Martín, Francisco
Suárez, Leonardo
Rosa, Alberto Luis
Morata, Ginés
author_role author
author2 Romero, Nuria Magdalena
Martín, Francisco
Suárez, Leonardo
Rosa, Alberto Luis
Morata, Ginés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
DROSOPHILA
MALE GENITAL DISC
PVF1/PVR
VEGF/PDGF
topic APOPTOSIS
DROSOPHILA
MALE GENITAL DISC
PVF1/PVR
VEGF/PDGF
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Drosophila adult male terminalia originate from the genital disc. During the pupal stages, the external parts of terminalia evert from two ventral stalks; the everted left and right dorsal halves fuse at the dorsal midline. At the same time the male terminalia perform a 360 clockwise rotation. Several mutations are known to affect the rotation of the male terminalia, while none is known to affect dorsal closure. We show here that the Pvf1 gene, encoding one of the three Drosophila homologues of the mammalian VEGF/PDGF growth factors, is required for both processes. Males either mutant for Pvf1 or bearing a dominant negative form of Pvr or stasis (stai), the unique PVF receptor, do not complete either rotation or dorsal closure. Pvf1 expression in the genital disc is restricted to the A8 cells. However, PVF1/PVR signaling influences A8, A9 and A10 cells, suggesting that the PVF1 protein diffuses from its source. Flies hemizygous for the apoptotic genes hid, reaper and grim, or mutant for puckered which encodes a phosphatase that down-regulates the n-Jun-N terminal kinase pathway, lead to the same phenotypes as mutations in PVF1/PVR. Our results indicate that PVF1/PVR signaling functions not only in apoptotic phenomena but are also required during rotation and dorsal closure of the Drosophila male genital disc.
Fil: Macias, Ana Maria. Universidad Nacional de Córdoba; Argentina
Fil: Romero, Nuria Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Martín, Francisco. Consejo Superior de Investigaciones Científicas; España
Fil: Suárez, Leonardo. Universidad Nacional de Córdoba; Argentina
Fil: Rosa, Alberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Morata, Ginés. Consejo Superior de Investigaciones Científicas; España
description The Drosophila adult male terminalia originate from the genital disc. During the pupal stages, the external parts of terminalia evert from two ventral stalks; the everted left and right dorsal halves fuse at the dorsal midline. At the same time the male terminalia perform a 360 clockwise rotation. Several mutations are known to affect the rotation of the male terminalia, while none is known to affect dorsal closure. We show here that the Pvf1 gene, encoding one of the three Drosophila homologues of the mammalian VEGF/PDGF growth factors, is required for both processes. Males either mutant for Pvf1 or bearing a dominant negative form of Pvr or stasis (stai), the unique PVF receptor, do not complete either rotation or dorsal closure. Pvf1 expression in the genital disc is restricted to the A8 cells. However, PVF1/PVR signaling influences A8, A9 and A10 cells, suggesting that the PVF1 protein diffuses from its source. Flies hemizygous for the apoptotic genes hid, reaper and grim, or mutant for puckered which encodes a phosphatase that down-regulates the n-Jun-N terminal kinase pathway, lead to the same phenotypes as mutations in PVF1/PVR. Our results indicate that PVF1/PVR signaling functions not only in apoptotic phenomena but are also required during rotation and dorsal closure of the Drosophila male genital disc.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96213
Macias, Ana Maria; Romero, Nuria Magdalena; Martín, Francisco; Suárez, Leonardo; Rosa, Alberto Luis; et al.; PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia; U B C Press; International Journal Of Developmental Biology; 48; 10; 12-2004; 1087-1094
0214-6282
1696-3547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96213
identifier_str_mv Macias, Ana Maria; Romero, Nuria Magdalena; Martín, Francisco; Suárez, Leonardo; Rosa, Alberto Luis; et al.; PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure of the Drosophila male terminalia; U B C Press; International Journal Of Developmental Biology; 48; 10; 12-2004; 1087-1094
0214-6282
1696-3547
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.041859am
info:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=10.1387/ijdb.041859am
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv U B C Press
publisher.none.fl_str_mv U B C Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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