A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern

Autores
Muñoz Nava, Luis Manuel; Alvarez, Hugo Ariel; Flores Flores, Marycruz; Chara, Osvaldo; Nahmad, Marcos
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. The overscaling of the Vg pattern cannot be explained by differential proliferation, apoptosis, or oriented-cell divisions, but can be recapitulated by a mathematical model that explicitly considers cell recruitment. When impairing cell recruitment genetically, we find that the Vg pattern almost perfectly scales and adult wings are approximately 20% smaller. Conversely, impairing cell proliferation results in very small wings, suggesting that cell recruitment and cell proliferation additively contribute to organ growth in this system. Furthermore, using fluorescent reporter tools, we provide direct evidence that cell recruitment is initiated between early and mid third-instar larval development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing.
Fil: Muñoz Nava, Luis Manuel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
Fil: Alvarez, Hugo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Flores Flores, Marycruz. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; Alemania
Fil: Nahmad, Marcos. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
Materia
ORGAN GROWTH
PATTERNING
VESTIGIAL
CELL RECRUITMENT
SCALING
DROSOPHILA WING DISC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154028
Acceder
id CONICETDig_5d5cdbfb76fdfb9999147b4563ac5016
oai_identifier_str oai:ri.conicet.gov.ar:11336/154028
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression patternMuñoz Nava, Luis ManuelAlvarez, Hugo ArielFlores Flores, MarycruzChara, OsvaldoNahmad, MarcosORGAN GROWTHPATTERNINGVESTIGIALCELL RECRUITMENTSCALINGDROSOPHILA WING DISChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. The overscaling of the Vg pattern cannot be explained by differential proliferation, apoptosis, or oriented-cell divisions, but can be recapitulated by a mathematical model that explicitly considers cell recruitment. When impairing cell recruitment genetically, we find that the Vg pattern almost perfectly scales and adult wings are approximately 20% smaller. Conversely, impairing cell proliferation results in very small wings, suggesting that cell recruitment and cell proliferation additively contribute to organ growth in this system. Furthermore, using fluorescent reporter tools, we provide direct evidence that cell recruitment is initiated between early and mid third-instar larval development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing.Fil: Muñoz Nava, Luis Manuel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; MéxicoFil: Alvarez, Hugo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; ArgentinaFil: Flores Flores, Marycruz. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; MéxicoFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; AlemaniaFil: Nahmad, Marcos. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; MéxicoAcademic Press Inc Elsevier Science2020-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154028Muñoz Nava, Luis Manuel; Alvarez, Hugo Ariel; Flores Flores, Marycruz; Chara, Osvaldo; Nahmad, Marcos; A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern; Academic Press Inc Elsevier Science; Developmental Biology; 462; 2; 15-6-2020; 141-1510012-1606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0012160620300919info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2020.03.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:39:03Zoai:ri.conicet.gov.ar:11336/154028instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:39:03.967CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
spellingShingle A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
Muñoz Nava, Luis Manuel
ORGAN GROWTH
PATTERNING
VESTIGIAL
CELL RECRUITMENT
SCALING
DROSOPHILA WING DISC
title_short A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_full A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_fullStr A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_full_unstemmed A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_sort A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
dc.creator.none.fl_str_mv Muñoz Nava, Luis Manuel
Alvarez, Hugo Ariel
Flores Flores, Marycruz
Chara, Osvaldo
Nahmad, Marcos
author Muñoz Nava, Luis Manuel
author_facet Muñoz Nava, Luis Manuel
Alvarez, Hugo Ariel
Flores Flores, Marycruz
Chara, Osvaldo
Nahmad, Marcos
author_role author
author2 Alvarez, Hugo Ariel
Flores Flores, Marycruz
Chara, Osvaldo
Nahmad, Marcos
author2_role author
author
author
author
dc.subject.none.fl_str_mv ORGAN GROWTH
PATTERNING
VESTIGIAL
CELL RECRUITMENT
SCALING
DROSOPHILA WING DISC
topic ORGAN GROWTH
PATTERNING
VESTIGIAL
CELL RECRUITMENT
SCALING
DROSOPHILA WING DISC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. The overscaling of the Vg pattern cannot be explained by differential proliferation, apoptosis, or oriented-cell divisions, but can be recapitulated by a mathematical model that explicitly considers cell recruitment. When impairing cell recruitment genetically, we find that the Vg pattern almost perfectly scales and adult wings are approximately 20% smaller. Conversely, impairing cell proliferation results in very small wings, suggesting that cell recruitment and cell proliferation additively contribute to organ growth in this system. Furthermore, using fluorescent reporter tools, we provide direct evidence that cell recruitment is initiated between early and mid third-instar larval development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing.
Fil: Muñoz Nava, Luis Manuel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
Fil: Alvarez, Hugo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Flores Flores, Marycruz. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; Alemania
Fil: Nahmad, Marcos. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional; México
description Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. The overscaling of the Vg pattern cannot be explained by differential proliferation, apoptosis, or oriented-cell divisions, but can be recapitulated by a mathematical model that explicitly considers cell recruitment. When impairing cell recruitment genetically, we find that the Vg pattern almost perfectly scales and adult wings are approximately 20% smaller. Conversely, impairing cell proliferation results in very small wings, suggesting that cell recruitment and cell proliferation additively contribute to organ growth in this system. Furthermore, using fluorescent reporter tools, we provide direct evidence that cell recruitment is initiated between early and mid third-instar larval development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154028
Muñoz Nava, Luis Manuel; Alvarez, Hugo Ariel; Flores Flores, Marycruz; Chara, Osvaldo; Nahmad, Marcos; A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern; Academic Press Inc Elsevier Science; Developmental Biology; 462; 2; 15-6-2020; 141-151
0012-1606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154028
identifier_str_mv Muñoz Nava, Luis Manuel; Alvarez, Hugo Ariel; Flores Flores, Marycruz; Chara, Osvaldo; Nahmad, Marcos; A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern; Academic Press Inc Elsevier Science; Developmental Biology; 462; 2; 15-6-2020; 141-151
0012-1606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0012160620300919
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2020.03.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782052366548992
score 12.982451

Publicaciones similares