Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development

Autores
Benitez, Sergio Gonzalo; Sosa, Claudia Marcela; Tomasini, Nicolás; Macías, Ana
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apoptosis is necessary for the regulation of growth during development, but the precise details of this regulation have not been completely characterized. In this study, we used the Drosophila genital disc as a model to analyze the contribution of apoptosis to growth regulation. We studied the expression or activity of several elements of the apoptotic death pathway such as Drosophila inhibitor of apoptosis1, caspases, the apoptotic genes reaper (rpr) and head involution defective, as well as elements of the Jun-NH2-terminal kinase (JNK) pathway. We found that the JNK pathway is active in a dynamic, asymmetric and genitalia-specific manner. Apoptosis, as measured in terms of the expression of a variety of apoptotic molecules, occurs in a JNK-dependent and -independent manner and was detected among engrailed (en) expressing cells. JNK regulation of apoptotic genes is necessary to control growth in both sexes and rotation in males; this regulatory role is necessary to execute en(+) cell death and to activate expression of rpr in these cells. rpr is up-regulated at antero-posterior borders, and this expression appears to be of particular importance in the control of growth, since the balance between cell proliferation and death in those regions appears to depend on the equilibrium between pro- and anti-apoptotic factors at a cellular level.
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Sosa, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Macías, Ana. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Materia
GENITAL DISC
JNK
APOPTOSIS
REAPER
DROSOPHILA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112105
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc developmentBenitez, Sergio GonzaloSosa, Claudia MarcelaTomasini, NicolásMacías, AnaGENITAL DISCJNKAPOPTOSISREAPERDROSOPHILAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apoptosis is necessary for the regulation of growth during development, but the precise details of this regulation have not been completely characterized. In this study, we used the Drosophila genital disc as a model to analyze the contribution of apoptosis to growth regulation. We studied the expression or activity of several elements of the apoptotic death pathway such as Drosophila inhibitor of apoptosis1, caspases, the apoptotic genes reaper (rpr) and head involution defective, as well as elements of the Jun-NH2-terminal kinase (JNK) pathway. We found that the JNK pathway is active in a dynamic, asymmetric and genitalia-specific manner. Apoptosis, as measured in terms of the expression of a variety of apoptotic molecules, occurs in a JNK-dependent and -independent manner and was detected among engrailed (en) expressing cells. JNK regulation of apoptotic genes is necessary to control growth in both sexes and rotation in males; this regulatory role is necessary to execute en(+) cell death and to activate expression of rpr in these cells. rpr is up-regulated at antero-posterior borders, and this expression appears to be of particular importance in the control of growth, since the balance between cell proliferation and death in those regions appears to depend on the equilibrium between pro- and anti-apoptotic factors at a cellular level.Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; ArgentinaFil: Sosa, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; ArgentinaFil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; ArgentinaFil: Macías, Ana. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; ArgentinaU B C Press2010-01-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112105Benitez, Sergio Gonzalo; Sosa, Claudia Marcela; Tomasini, Nicolás; Macías, Ana; Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development; U B C Press; International Journal Of Developmental Biology; 54; 29-1-2010; 643-6530214-62821696-3547CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=10.1387/ijdb.082724sbinfo:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.082724sbinfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20209437/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:29Zoai:ri.conicet.gov.ar:11336/112105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:29.89CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
title Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
spellingShingle Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
Benitez, Sergio Gonzalo
GENITAL DISC
JNK
APOPTOSIS
REAPER
DROSOPHILA
title_short Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
title_full Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
title_fullStr Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
title_full_unstemmed Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
title_sort Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development
dc.creator.none.fl_str_mv Benitez, Sergio Gonzalo
Sosa, Claudia Marcela
Tomasini, Nicolás
Macías, Ana
author Benitez, Sergio Gonzalo
author_facet Benitez, Sergio Gonzalo
Sosa, Claudia Marcela
Tomasini, Nicolás
Macías, Ana
author_role author
author2 Sosa, Claudia Marcela
Tomasini, Nicolás
Macías, Ana
author2_role author
author
author
dc.subject.none.fl_str_mv GENITAL DISC
JNK
APOPTOSIS
REAPER
DROSOPHILA
topic GENITAL DISC
JNK
APOPTOSIS
REAPER
DROSOPHILA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apoptosis is necessary for the regulation of growth during development, but the precise details of this regulation have not been completely characterized. In this study, we used the Drosophila genital disc as a model to analyze the contribution of apoptosis to growth regulation. We studied the expression or activity of several elements of the apoptotic death pathway such as Drosophila inhibitor of apoptosis1, caspases, the apoptotic genes reaper (rpr) and head involution defective, as well as elements of the Jun-NH2-terminal kinase (JNK) pathway. We found that the JNK pathway is active in a dynamic, asymmetric and genitalia-specific manner. Apoptosis, as measured in terms of the expression of a variety of apoptotic molecules, occurs in a JNK-dependent and -independent manner and was detected among engrailed (en) expressing cells. JNK regulation of apoptotic genes is necessary to control growth in both sexes and rotation in males; this regulatory role is necessary to execute en(+) cell death and to activate expression of rpr in these cells. rpr is up-regulated at antero-posterior borders, and this expression appears to be of particular importance in the control of growth, since the balance between cell proliferation and death in those regions appears to depend on the equilibrium between pro- and anti-apoptotic factors at a cellular level.
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Sosa, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
Fil: Macías, Ana. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología. Laboratorio de Genética del Desarrollo; Argentina
description Apoptosis is necessary for the regulation of growth during development, but the precise details of this regulation have not been completely characterized. In this study, we used the Drosophila genital disc as a model to analyze the contribution of apoptosis to growth regulation. We studied the expression or activity of several elements of the apoptotic death pathway such as Drosophila inhibitor of apoptosis1, caspases, the apoptotic genes reaper (rpr) and head involution defective, as well as elements of the Jun-NH2-terminal kinase (JNK) pathway. We found that the JNK pathway is active in a dynamic, asymmetric and genitalia-specific manner. Apoptosis, as measured in terms of the expression of a variety of apoptotic molecules, occurs in a JNK-dependent and -independent manner and was detected among engrailed (en) expressing cells. JNK regulation of apoptotic genes is necessary to control growth in both sexes and rotation in males; this regulatory role is necessary to execute en(+) cell death and to activate expression of rpr in these cells. rpr is up-regulated at antero-posterior borders, and this expression appears to be of particular importance in the control of growth, since the balance between cell proliferation and death in those regions appears to depend on the equilibrium between pro- and anti-apoptotic factors at a cellular level.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112105
Benitez, Sergio Gonzalo; Sosa, Claudia Marcela; Tomasini, Nicolás; Macías, Ana; Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development; U B C Press; International Journal Of Developmental Biology; 54; 29-1-2010; 643-653
0214-6282
1696-3547
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112105
identifier_str_mv Benitez, Sergio Gonzalo; Sosa, Claudia Marcela; Tomasini, Nicolás; Macías, Ana; Both JNK and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital disc development; U B C Press; International Journal Of Developmental Biology; 54; 29-1-2010; 643-653
0214-6282
1696-3547
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ijdb.ehu.es/web/paper.php?doi=10.1387/ijdb.082724sb
info:eu-repo/semantics/altIdentifier/doi/10.1387/ijdb.082724sb
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/20209437/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv U B C Press
publisher.none.fl_str_mv U B C Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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