Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation

Autores
Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Ma, Kit-Wan. The Chinese University of Hong Kong; China
Fil: Shaw, Pang-Chui. The Chinese University of Hong Kong; China
Fil: Wong, Kam-Bo. The Chinese University of Hong Kong; China
Materia
CHAGAS DISEASE
PROTEIN SYNTHESIS
RIBOSOME-INACTIVATING PROTEIN (RIP)
TRICHOSANTHIN
TRYPANOSOMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125061
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivationJuri Ayub, MaximilianoMa, Kit-WanShaw, Pang-ChuiWong, Kam-BoCHAGAS DISEASEPROTEIN SYNTHESISRIBOSOME-INACTIVATING PROTEIN (RIP)TRICHOSANTHINTRYPANOSOMAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: Ma, Kit-Wan. The Chinese University of Hong Kong; ChinaFil: Shaw, Pang-Chui. The Chinese University of Hong Kong; ChinaFil: Wong, Kam-Bo. The Chinese University of Hong Kong; ChinaAcademic Press Inc Elsevier Science2008-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125061Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo; Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation; Academic Press Inc Elsevier Science; Experimental Parasitology; 118; 3; 3-2008; 442-4470014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0014489407002408info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2007.09.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:44Zoai:ri.conicet.gov.ar:11336/125061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:44.853CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
title Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
spellingShingle Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
Juri Ayub, Maximiliano
CHAGAS DISEASE
PROTEIN SYNTHESIS
RIBOSOME-INACTIVATING PROTEIN (RIP)
TRICHOSANTHIN
TRYPANOSOMA
title_short Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
title_full Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
title_fullStr Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
title_full_unstemmed Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
title_sort Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
dc.creator.none.fl_str_mv Juri Ayub, Maximiliano
Ma, Kit-Wan
Shaw, Pang-Chui
Wong, Kam-Bo
author Juri Ayub, Maximiliano
author_facet Juri Ayub, Maximiliano
Ma, Kit-Wan
Shaw, Pang-Chui
Wong, Kam-Bo
author_role author
author2 Ma, Kit-Wan
Shaw, Pang-Chui
Wong, Kam-Bo
author2_role author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
PROTEIN SYNTHESIS
RIBOSOME-INACTIVATING PROTEIN (RIP)
TRICHOSANTHIN
TRYPANOSOMA
topic CHAGAS DISEASE
PROTEIN SYNTHESIS
RIBOSOME-INACTIVATING PROTEIN (RIP)
TRICHOSANTHIN
TRYPANOSOMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Ma, Kit-Wan. The Chinese University of Hong Kong; China
Fil: Shaw, Pang-Chui. The Chinese University of Hong Kong; China
Fil: Wong, Kam-Bo. The Chinese University of Hong Kong; China
description Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.
publishDate 2008
dc.date.none.fl_str_mv 2008-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125061
Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo; Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation; Academic Press Inc Elsevier Science; Experimental Parasitology; 118; 3; 3-2008; 442-447
0014-4894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125061
identifier_str_mv Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo; Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation; Academic Press Inc Elsevier Science; Experimental Parasitology; 118; 3; 3-2008; 442-447
0014-4894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0014489407002408
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2007.09.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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