Proteomic analysis of the Trypanosoma cruzi ribosomal proteins

Autores
Juri Ayub, Maximiliano; Atwood, James; Nuccio, Arthur; Tarleton, Rick; Levin, Mariano Jorge
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi is a parasite responsible for Chagas disease. The identification of new targets for chemotherapy is a major challenge for the control of this disease. Several lines of evidences suggest that the translational system in trypanosomatids show important differences compared to other eukaryotes. However, there little is known information about this. We have performed a detailed data mining search for ribosomal protein genes in T. cruzi genome data base combined with mass spectrometry analysis of purified T. cruzi ribosomes. Our results show that T. cruzi ribosomal proteins have ∼50% sequence identity to yeast ones. Nevertheless, some parasite proteins are longer due to the presence of several N- or C-terminal extensions, which are exclusive of trypanosomatids. In particular, L19 and S21 show C-terminal extensions of 168 and 164 amino acids, respectively. In addition, we detected two 60S subunit proteins that had not been previously detected in the T. cruzi total proteome; namely, L22 and L42.
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Atwood, James. Georgia State University; Estados Unidos
Fil: Nuccio, Arthur. Georgia State University; Estados Unidos
Fil: Tarleton, Rick. Georgia State University; Estados Unidos
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
MASS SPECTROMETRY
PROTEIN SYNTHESIS
RIBOSOME
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130750

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network_name_str CONICET Digital (CONICET)
spelling Proteomic analysis of the Trypanosoma cruzi ribosomal proteinsJuri Ayub, MaximilianoAtwood, JamesNuccio, ArthurTarleton, RickLevin, Mariano JorgeMASS SPECTROMETRYPROTEIN SYNTHESISRIBOSOMETRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is a parasite responsible for Chagas disease. The identification of new targets for chemotherapy is a major challenge for the control of this disease. Several lines of evidences suggest that the translational system in trypanosomatids show important differences compared to other eukaryotes. However, there little is known information about this. We have performed a detailed data mining search for ribosomal protein genes in T. cruzi genome data base combined with mass spectrometry analysis of purified T. cruzi ribosomes. Our results show that T. cruzi ribosomal proteins have ∼50% sequence identity to yeast ones. Nevertheless, some parasite proteins are longer due to the presence of several N- or C-terminal extensions, which are exclusive of trypanosomatids. In particular, L19 and S21 show C-terminal extensions of 168 and 164 amino acids, respectively. In addition, we detected two 60S subunit proteins that had not been previously detected in the T. cruzi total proteome; namely, L22 and L42.Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Atwood, James. Georgia State University; Estados UnidosFil: Nuccio, Arthur. Georgia State University; Estados UnidosFil: Tarleton, Rick. Georgia State University; Estados UnidosFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaAcademic Press Inc Elsevier Science2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130750Juri Ayub, Maximiliano; Atwood, James; Nuccio, Arthur; Tarleton, Rick; Levin, Mariano Jorge; Proteomic analysis of the Trypanosoma cruzi ribosomal proteins; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 382; 1; 2-2009; 30-340006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0006291X0900360Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.02.095info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:36Zoai:ri.conicet.gov.ar:11336/130750instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:36.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
title Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
spellingShingle Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
Juri Ayub, Maximiliano
MASS SPECTROMETRY
PROTEIN SYNTHESIS
RIBOSOME
TRYPANOSOMA CRUZI
title_short Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
title_full Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
title_fullStr Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
title_full_unstemmed Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
title_sort Proteomic analysis of the Trypanosoma cruzi ribosomal proteins
dc.creator.none.fl_str_mv Juri Ayub, Maximiliano
Atwood, James
Nuccio, Arthur
Tarleton, Rick
Levin, Mariano Jorge
author Juri Ayub, Maximiliano
author_facet Juri Ayub, Maximiliano
Atwood, James
Nuccio, Arthur
Tarleton, Rick
Levin, Mariano Jorge
author_role author
author2 Atwood, James
Nuccio, Arthur
Tarleton, Rick
Levin, Mariano Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv MASS SPECTROMETRY
PROTEIN SYNTHESIS
RIBOSOME
TRYPANOSOMA CRUZI
topic MASS SPECTROMETRY
PROTEIN SYNTHESIS
RIBOSOME
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi is a parasite responsible for Chagas disease. The identification of new targets for chemotherapy is a major challenge for the control of this disease. Several lines of evidences suggest that the translational system in trypanosomatids show important differences compared to other eukaryotes. However, there little is known information about this. We have performed a detailed data mining search for ribosomal protein genes in T. cruzi genome data base combined with mass spectrometry analysis of purified T. cruzi ribosomes. Our results show that T. cruzi ribosomal proteins have ∼50% sequence identity to yeast ones. Nevertheless, some parasite proteins are longer due to the presence of several N- or C-terminal extensions, which are exclusive of trypanosomatids. In particular, L19 and S21 show C-terminal extensions of 168 and 164 amino acids, respectively. In addition, we detected two 60S subunit proteins that had not been previously detected in the T. cruzi total proteome; namely, L22 and L42.
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Atwood, James. Georgia State University; Estados Unidos
Fil: Nuccio, Arthur. Georgia State University; Estados Unidos
Fil: Tarleton, Rick. Georgia State University; Estados Unidos
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description Trypanosoma cruzi is a parasite responsible for Chagas disease. The identification of new targets for chemotherapy is a major challenge for the control of this disease. Several lines of evidences suggest that the translational system in trypanosomatids show important differences compared to other eukaryotes. However, there little is known information about this. We have performed a detailed data mining search for ribosomal protein genes in T. cruzi genome data base combined with mass spectrometry analysis of purified T. cruzi ribosomes. Our results show that T. cruzi ribosomal proteins have ∼50% sequence identity to yeast ones. Nevertheless, some parasite proteins are longer due to the presence of several N- or C-terminal extensions, which are exclusive of trypanosomatids. In particular, L19 and S21 show C-terminal extensions of 168 and 164 amino acids, respectively. In addition, we detected two 60S subunit proteins that had not been previously detected in the T. cruzi total proteome; namely, L22 and L42.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130750
Juri Ayub, Maximiliano; Atwood, James; Nuccio, Arthur; Tarleton, Rick; Levin, Mariano Jorge; Proteomic analysis of the Trypanosoma cruzi ribosomal proteins; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 382; 1; 2-2009; 30-34
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130750
identifier_str_mv Juri Ayub, Maximiliano; Atwood, James; Nuccio, Arthur; Tarleton, Rick; Levin, Mariano Jorge; Proteomic analysis of the Trypanosoma cruzi ribosomal proteins; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 382; 1; 2-2009; 30-34
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0006291X0900360X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.02.095
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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