Challenges in Identifying the Dark Molecules of Life

Autores
Monge, Maria Eugenia; Dodds, James N.; Baker, Erin S.; Edison, Arthur Scott; Fernández, Facundo M.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metabolomics is the study of the metabolome, the collection of small molecules in living organisms, cells, tissues, and biofluids. Technological advances in mass spectrometry, liquid- and gas-phase separations, nuclear magnetic resonance spectroscopy, and big data analytics have now made it possible to study metabolism at an omics or systems level. The significance of this burgeoning scientific field cannot be overstated: It impacts disciplines ranging from biomedicine to plant science. Despite these advances, the central bottleneck in metabolomics remains the identification of key metabolites that play a class-discriminant role. Because metabolites do not follow a molecular alphabet as proteins and nucleic acids do, their identification is much more time consuming, with a high failure rate. In this review, we critically discuss the state-of-the-art in metabolite identification with specific applications in metabolomics and how technologies such as mass spectrometry, ion mobility, chromatography, and nuclear magnetic resonance currently contribute to this challenging task.
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Dodds, James N.. North Carolina State University; Estados Unidos
Fil: Baker, Erin S.. North Carolina State University; Estados Unidos
Fil: Edison, Arthur Scott. University of Georgia; Estados Unidos
Fil: Fernández, Facundo M.. Georgia Institute Of Technology; Estados Unidos. Petit Institute for Biochemistry and Bioscience; Estados Unidos
Materia
METABOLOMICS
METABOLITE IDENTIFICATION
CHROMATOGRAPHY
ION MOBILITY
TANDEM MASS SPECTROMETRY
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108448
Acceder
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spelling Challenges in Identifying the Dark Molecules of LifeMonge, Maria EugeniaDodds, James N.Baker, Erin S.Edison, Arthur ScottFernández, Facundo M.METABOLOMICSMETABOLITE IDENTIFICATIONCHROMATOGRAPHYION MOBILITYTANDEM MASS SPECTROMETRYNUCLEAR MAGNETIC RESONANCE SPECTROSCOPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metabolomics is the study of the metabolome, the collection of small molecules in living organisms, cells, tissues, and biofluids. Technological advances in mass spectrometry, liquid- and gas-phase separations, nuclear magnetic resonance spectroscopy, and big data analytics have now made it possible to study metabolism at an omics or systems level. The significance of this burgeoning scientific field cannot be overstated: It impacts disciplines ranging from biomedicine to plant science. Despite these advances, the central bottleneck in metabolomics remains the identification of key metabolites that play a class-discriminant role. Because metabolites do not follow a molecular alphabet as proteins and nucleic acids do, their identification is much more time consuming, with a high failure rate. In this review, we critically discuss the state-of-the-art in metabolite identification with specific applications in metabolomics and how technologies such as mass spectrometry, ion mobility, chromatography, and nuclear magnetic resonance currently contribute to this challenging task.Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Dodds, James N.. North Carolina State University; Estados UnidosFil: Baker, Erin S.. North Carolina State University; Estados UnidosFil: Edison, Arthur Scott. University of Georgia; Estados UnidosFil: Fernández, Facundo M.. Georgia Institute Of Technology; Estados Unidos. Petit Institute for Biochemistry and Bioscience; Estados UnidosAnnual Reviews2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108448Monge, Maria Eugenia; Dodds, James N.; Baker, Erin S.; Edison, Arthur Scott; Fernández, Facundo M.; Challenges in Identifying the Dark Molecules of Life; Annual Reviews; Annual Review Of Analytical Chemistry; 12; 1; 3-2019; 177-1991936-1327CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.annualreviews.org/doi/10.1146/annurev-anchem-061318-114959info:eu-repo/semantics/altIdentifier/doi/10.1146/annurev-anchem-061318-114959info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716371/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:00Zoai:ri.conicet.gov.ar:11336/108448instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:00.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Challenges in Identifying the Dark Molecules of Life
title Challenges in Identifying the Dark Molecules of Life
spellingShingle Challenges in Identifying the Dark Molecules of Life
Monge, Maria Eugenia
METABOLOMICS
METABOLITE IDENTIFICATION
CHROMATOGRAPHY
ION MOBILITY
TANDEM MASS SPECTROMETRY
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
title_short Challenges in Identifying the Dark Molecules of Life
title_full Challenges in Identifying the Dark Molecules of Life
title_fullStr Challenges in Identifying the Dark Molecules of Life
title_full_unstemmed Challenges in Identifying the Dark Molecules of Life
title_sort Challenges in Identifying the Dark Molecules of Life
dc.creator.none.fl_str_mv Monge, Maria Eugenia
Dodds, James N.
Baker, Erin S.
Edison, Arthur Scott
Fernández, Facundo M.
author Monge, Maria Eugenia
author_facet Monge, Maria Eugenia
Dodds, James N.
Baker, Erin S.
Edison, Arthur Scott
Fernández, Facundo M.
author_role author
author2 Dodds, James N.
Baker, Erin S.
Edison, Arthur Scott
Fernández, Facundo M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv METABOLOMICS
METABOLITE IDENTIFICATION
CHROMATOGRAPHY
ION MOBILITY
TANDEM MASS SPECTROMETRY
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
topic METABOLOMICS
METABOLITE IDENTIFICATION
CHROMATOGRAPHY
ION MOBILITY
TANDEM MASS SPECTROMETRY
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Metabolomics is the study of the metabolome, the collection of small molecules in living organisms, cells, tissues, and biofluids. Technological advances in mass spectrometry, liquid- and gas-phase separations, nuclear magnetic resonance spectroscopy, and big data analytics have now made it possible to study metabolism at an omics or systems level. The significance of this burgeoning scientific field cannot be overstated: It impacts disciplines ranging from biomedicine to plant science. Despite these advances, the central bottleneck in metabolomics remains the identification of key metabolites that play a class-discriminant role. Because metabolites do not follow a molecular alphabet as proteins and nucleic acids do, their identification is much more time consuming, with a high failure rate. In this review, we critically discuss the state-of-the-art in metabolite identification with specific applications in metabolomics and how technologies such as mass spectrometry, ion mobility, chromatography, and nuclear magnetic resonance currently contribute to this challenging task.
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Dodds, James N.. North Carolina State University; Estados Unidos
Fil: Baker, Erin S.. North Carolina State University; Estados Unidos
Fil: Edison, Arthur Scott. University of Georgia; Estados Unidos
Fil: Fernández, Facundo M.. Georgia Institute Of Technology; Estados Unidos. Petit Institute for Biochemistry and Bioscience; Estados Unidos
description Metabolomics is the study of the metabolome, the collection of small molecules in living organisms, cells, tissues, and biofluids. Technological advances in mass spectrometry, liquid- and gas-phase separations, nuclear magnetic resonance spectroscopy, and big data analytics have now made it possible to study metabolism at an omics or systems level. The significance of this burgeoning scientific field cannot be overstated: It impacts disciplines ranging from biomedicine to plant science. Despite these advances, the central bottleneck in metabolomics remains the identification of key metabolites that play a class-discriminant role. Because metabolites do not follow a molecular alphabet as proteins and nucleic acids do, their identification is much more time consuming, with a high failure rate. In this review, we critically discuss the state-of-the-art in metabolite identification with specific applications in metabolomics and how technologies such as mass spectrometry, ion mobility, chromatography, and nuclear magnetic resonance currently contribute to this challenging task.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108448
Monge, Maria Eugenia; Dodds, James N.; Baker, Erin S.; Edison, Arthur Scott; Fernández, Facundo M.; Challenges in Identifying the Dark Molecules of Life; Annual Reviews; Annual Review Of Analytical Chemistry; 12; 1; 3-2019; 177-199
1936-1327
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108448
identifier_str_mv Monge, Maria Eugenia; Dodds, James N.; Baker, Erin S.; Edison, Arthur Scott; Fernández, Facundo M.; Challenges in Identifying the Dark Molecules of Life; Annual Reviews; Annual Review Of Analytical Chemistry; 12; 1; 3-2019; 177-199
1936-1327
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.annualreviews.org/doi/10.1146/annurev-anchem-061318-114959
info:eu-repo/semantics/altIdentifier/doi/10.1146/annurev-anchem-061318-114959
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716371/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Annual Reviews
publisher.none.fl_str_mv Annual Reviews
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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