Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina

Autores
Quiroga, Gisela Alejandra Ramona; Wright, Eduardo Roberto; Herrera, O.; Silvestro, Luciana Belén; Stenglein, Sebastian Alberto; Rivera, M. C.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In July 2015, bulbs of Hippeastrum × hybridum imported for cultivation in Buenos Aires (Argentina) showed loss of firmness, rot and green mold. Small pieces from the edge of the lesions were surface disinfected with 2% sodium hypochlorite for 90 s, rinsed in sterilized distilled water and incubated on potato dextrose agar at 22°C. Isolates of Penicillium brevicompactum Dierckx (Samson and Frisvad, 2004) were consistently obtained, and one of them coded INTA-IF-502 was chosen to confirm pathogenicity. Healthy, unwounded and needle-punctured bulbs were immersed for 1 min in suspensions of 1.4 × 106 conidia ml-1, while those immersed in sterilized water served as controls. The bulbs were individually planted in pots filled with a sterile soil/perlite mixture (80/20), and enclosed in polyethylene bags for the first three days. All the plants from inoculated bulbs showed yellowish foliage and loss of turgidity in 45 days. Ten days after, the plants from inoculated punctured bulbs had died and those from inoculated unwounded bulbs showed an advanced wilt. Rotten bulbs and roots, and green mold were observed on inoculated plants eased out of the pots whereas controls remained healthy. The pathogen was successfully recovered from infected organs and deposited in La Plata Spegazzini Collection as LPSC1146. The morphological identification (Visagie et al., 2014) was confirmed by DNA sequence data of the β-tubulin gene (GenBank accession No. KY216143) showing 99% identity to accession AY674437 (type of P. brevicompactum CBS 257.29). This finding represents the first record of P. brevicompactum associated with bulb rot of Hippeastrum × hybridum in Argentina.
Fil: Quiroga, Gisela Alejandra Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Wright, Eduardo Roberto. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Herrera, O.. No especifica;
Fil: Silvestro, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Rivera, M. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Materia
Bulb
Pathogen
Fungi
Penicillium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78509
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78509
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in ArgentinaQuiroga, Gisela Alejandra RamonaWright, Eduardo RobertoHerrera, O.Silvestro, Luciana BelénStenglein, Sebastian AlbertoRivera, M. C.BulbPathogenFungiPenicilliumhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4In July 2015, bulbs of Hippeastrum × hybridum imported for cultivation in Buenos Aires (Argentina) showed loss of firmness, rot and green mold. Small pieces from the edge of the lesions were surface disinfected with 2% sodium hypochlorite for 90 s, rinsed in sterilized distilled water and incubated on potato dextrose agar at 22°C. Isolates of Penicillium brevicompactum Dierckx (Samson and Frisvad, 2004) were consistently obtained, and one of them coded INTA-IF-502 was chosen to confirm pathogenicity. Healthy, unwounded and needle-punctured bulbs were immersed for 1 min in suspensions of 1.4 × 106 conidia ml-1, while those immersed in sterilized water served as controls. The bulbs were individually planted in pots filled with a sterile soil/perlite mixture (80/20), and enclosed in polyethylene bags for the first three days. All the plants from inoculated bulbs showed yellowish foliage and loss of turgidity in 45 days. Ten days after, the plants from inoculated punctured bulbs had died and those from inoculated unwounded bulbs showed an advanced wilt. Rotten bulbs and roots, and green mold were observed on inoculated plants eased out of the pots whereas controls remained healthy. The pathogen was successfully recovered from infected organs and deposited in La Plata Spegazzini Collection as LPSC1146. The morphological identification (Visagie et al., 2014) was confirmed by DNA sequence data of the β-tubulin gene (GenBank accession No. KY216143) showing 99% identity to accession AY674437 (type of P. brevicompactum CBS 257.29). This finding represents the first record of P. brevicompactum associated with bulb rot of Hippeastrum × hybridum in Argentina.Fil: Quiroga, Gisela Alejandra Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Wright, Eduardo Roberto. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Herrera, O.. No especifica;Fil: Silvestro, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Rivera, M. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaEdizioni Ets2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78509Quiroga, Gisela Alejandra Ramona; Wright, Eduardo Roberto; Herrera, O.; Silvestro, Luciana Belén; Stenglein, Sebastian Alberto; et al.; Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 5-2017; 8011125-4653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3928info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i3.3928info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:34Zoai:ri.conicet.gov.ar:11336/78509instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:34.488CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
title Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
spellingShingle Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
Quiroga, Gisela Alejandra Ramona
Bulb
Pathogen
Fungi
Penicillium
title_short Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
title_full Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
title_fullStr Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
title_full_unstemmed Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
title_sort Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina
dc.creator.none.fl_str_mv Quiroga, Gisela Alejandra Ramona
Wright, Eduardo Roberto
Herrera, O.
Silvestro, Luciana Belén
Stenglein, Sebastian Alberto
Rivera, M. C.
author Quiroga, Gisela Alejandra Ramona
author_facet Quiroga, Gisela Alejandra Ramona
Wright, Eduardo Roberto
Herrera, O.
Silvestro, Luciana Belén
Stenglein, Sebastian Alberto
Rivera, M. C.
author_role author
author2 Wright, Eduardo Roberto
Herrera, O.
Silvestro, Luciana Belén
Stenglein, Sebastian Alberto
Rivera, M. C.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bulb
Pathogen
Fungi
Penicillium
topic Bulb
Pathogen
Fungi
Penicillium
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In July 2015, bulbs of Hippeastrum × hybridum imported for cultivation in Buenos Aires (Argentina) showed loss of firmness, rot and green mold. Small pieces from the edge of the lesions were surface disinfected with 2% sodium hypochlorite for 90 s, rinsed in sterilized distilled water and incubated on potato dextrose agar at 22°C. Isolates of Penicillium brevicompactum Dierckx (Samson and Frisvad, 2004) were consistently obtained, and one of them coded INTA-IF-502 was chosen to confirm pathogenicity. Healthy, unwounded and needle-punctured bulbs were immersed for 1 min in suspensions of 1.4 × 106 conidia ml-1, while those immersed in sterilized water served as controls. The bulbs were individually planted in pots filled with a sterile soil/perlite mixture (80/20), and enclosed in polyethylene bags for the first three days. All the plants from inoculated bulbs showed yellowish foliage and loss of turgidity in 45 days. Ten days after, the plants from inoculated punctured bulbs had died and those from inoculated unwounded bulbs showed an advanced wilt. Rotten bulbs and roots, and green mold were observed on inoculated plants eased out of the pots whereas controls remained healthy. The pathogen was successfully recovered from infected organs and deposited in La Plata Spegazzini Collection as LPSC1146. The morphological identification (Visagie et al., 2014) was confirmed by DNA sequence data of the β-tubulin gene (GenBank accession No. KY216143) showing 99% identity to accession AY674437 (type of P. brevicompactum CBS 257.29). This finding represents the first record of P. brevicompactum associated with bulb rot of Hippeastrum × hybridum in Argentina.
Fil: Quiroga, Gisela Alejandra Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Wright, Eduardo Roberto. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Herrera, O.. No especifica;
Fil: Silvestro, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina
Fil: Rivera, M. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
description In July 2015, bulbs of Hippeastrum × hybridum imported for cultivation in Buenos Aires (Argentina) showed loss of firmness, rot and green mold. Small pieces from the edge of the lesions were surface disinfected with 2% sodium hypochlorite for 90 s, rinsed in sterilized distilled water and incubated on potato dextrose agar at 22°C. Isolates of Penicillium brevicompactum Dierckx (Samson and Frisvad, 2004) were consistently obtained, and one of them coded INTA-IF-502 was chosen to confirm pathogenicity. Healthy, unwounded and needle-punctured bulbs were immersed for 1 min in suspensions of 1.4 × 106 conidia ml-1, while those immersed in sterilized water served as controls. The bulbs were individually planted in pots filled with a sterile soil/perlite mixture (80/20), and enclosed in polyethylene bags for the first three days. All the plants from inoculated bulbs showed yellowish foliage and loss of turgidity in 45 days. Ten days after, the plants from inoculated punctured bulbs had died and those from inoculated unwounded bulbs showed an advanced wilt. Rotten bulbs and roots, and green mold were observed on inoculated plants eased out of the pots whereas controls remained healthy. The pathogen was successfully recovered from infected organs and deposited in La Plata Spegazzini Collection as LPSC1146. The morphological identification (Visagie et al., 2014) was confirmed by DNA sequence data of the β-tubulin gene (GenBank accession No. KY216143) showing 99% identity to accession AY674437 (type of P. brevicompactum CBS 257.29). This finding represents the first record of P. brevicompactum associated with bulb rot of Hippeastrum × hybridum in Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78509
Quiroga, Gisela Alejandra Ramona; Wright, Eduardo Roberto; Herrera, O.; Silvestro, Luciana Belén; Stenglein, Sebastian Alberto; et al.; Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 5-2017; 801
1125-4653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78509
identifier_str_mv Quiroga, Gisela Alejandra Ramona; Wright, Eduardo Roberto; Herrera, O.; Silvestro, Luciana Belén; Stenglein, Sebastian Alberto; et al.; Penicillium Brevicompactum causing bulb rot on Hippeastrum × Hybridum in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 3; 5-2017; 801
1125-4653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3928
info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i3.3928
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Edizioni Ets
publisher.none.fl_str_mv Edizioni Ets
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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