Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infe...

Autores
Parreño, Gladys Viviana; Bai, Muqun; Liu, Fangning; Jing, Jiqiang; Olney, Erika; Li, Guohua; Wen, Ke; Yang, Xingdong; Castellucc, Tammy Bui; Kocher, Jacob F.; Zhou, Xu; Yuan, Lijuan
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective.
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bai, Muqun. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Liu, Fangning. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Jing, Jiqiang. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Olney, Erika. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Li, Guohua. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Wen, Ke. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Yang, Xingdong. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Castellucc, Tammy Bui. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Kocher, Jacob F.. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Zhou, Xu. Lanzhou Institute of Biological Products; China
Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Materia
GNOTOBIOTIC PIG
LACTOBACILLUS RHAMNOSUS GG
LANZHOU TRIVALENT VACCINE
PROBIOTIC ADJUVANT
ROTAVIRUS VACCINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210364
Acceder
id CONICETDig_74a85a848d8477044584bf551749c6dd
oai_identifier_str oai:ri.conicet.gov.ar:11336/210364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and DiseaseParreño, Gladys VivianaBai, MuqunLiu, FangningJing, JiqiangOlney, ErikaLi, GuohuaWen, KeYang, XingdongCastellucc, Tammy BuiKocher, Jacob F.Zhou, XuYuan, LijuanGNOTOBIOTIC PIGLACTOBACILLUS RHAMNOSUS GGLANZHOU TRIVALENT VACCINEPROBIOTIC ADJUVANTROTAVIRUS VACCINEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective.Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Bai, Muqun. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Liu, Fangning. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Jing, Jiqiang. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Olney, Erika. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Li, Guohua. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Wen, Ke. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Yang, Xingdong. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Castellucc, Tammy Bui. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Kocher, Jacob F.. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Zhou, Xu. Lanzhou Institute of Biological Products; ChinaFil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados UnidosMDPI2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210364Parreño, Gladys Viviana; Bai, Muqun; Liu, Fangning; Jing, Jiqiang; Olney, Erika; et al.; Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease; MDPI; Vaccines; 10; 9; 9-2022; 1-222076-393XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines10091529info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:50Zoai:ri.conicet.gov.ar:11336/210364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:50.585CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
title Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
spellingShingle Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
Parreño, Gladys Viviana
GNOTOBIOTIC PIG
LACTOBACILLUS RHAMNOSUS GG
LANZHOU TRIVALENT VACCINE
PROBIOTIC ADJUVANT
ROTAVIRUS VACCINE
title_short Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
title_full Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
title_fullStr Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
title_full_unstemmed Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
title_sort Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease
dc.creator.none.fl_str_mv Parreño, Gladys Viviana
Bai, Muqun
Liu, Fangning
Jing, Jiqiang
Olney, Erika
Li, Guohua
Wen, Ke
Yang, Xingdong
Castellucc, Tammy Bui
Kocher, Jacob F.
Zhou, Xu
Yuan, Lijuan
author Parreño, Gladys Viviana
author_facet Parreño, Gladys Viviana
Bai, Muqun
Liu, Fangning
Jing, Jiqiang
Olney, Erika
Li, Guohua
Wen, Ke
Yang, Xingdong
Castellucc, Tammy Bui
Kocher, Jacob F.
Zhou, Xu
Yuan, Lijuan
author_role author
author2 Bai, Muqun
Liu, Fangning
Jing, Jiqiang
Olney, Erika
Li, Guohua
Wen, Ke
Yang, Xingdong
Castellucc, Tammy Bui
Kocher, Jacob F.
Zhou, Xu
Yuan, Lijuan
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GNOTOBIOTIC PIG
LACTOBACILLUS RHAMNOSUS GG
LANZHOU TRIVALENT VACCINE
PROBIOTIC ADJUVANT
ROTAVIRUS VACCINE
topic GNOTOBIOTIC PIG
LACTOBACILLUS RHAMNOSUS GG
LANZHOU TRIVALENT VACCINE
PROBIOTIC ADJUVANT
ROTAVIRUS VACCINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective.
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bai, Muqun. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Liu, Fangning. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Jing, Jiqiang. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Olney, Erika. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Li, Guohua. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Wen, Ke. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Yang, Xingdong. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Castellucc, Tammy Bui. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Kocher, Jacob F.. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Zhou, Xu. Lanzhou Institute of Biological Products; China
Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
description This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210364
Parreño, Gladys Viviana; Bai, Muqun; Liu, Fangning; Jing, Jiqiang; Olney, Erika; et al.; Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease; MDPI; Vaccines; 10; 9; 9-2022; 1-22
2076-393X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210364
identifier_str_mv Parreño, Gladys Viviana; Bai, Muqun; Liu, Fangning; Jing, Jiqiang; Olney, Erika; et al.; Probiotic as Adjuvant Significantly Improves Protection of the Lanzhou Trivalent Rotavirus Vaccine against Heterologous Challenge in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease; MDPI; Vaccines; 10; 9; 9-2022; 1-22
2076-393X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines10091529
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268997854167040
score 13.13397

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