Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G...

Autores
Hensley, Casey; Nyblade, Charlotte; Zhou, Peng; Parreño, Gladys Viviana; Ramesh, Ashwin; Frazier, Annie; Frazier, Maggie; Garrison, Sarah; Fantasia Davis, Ariana; Cai, Ruiqing; Huang, Peng Wei; Xia, Ming; Tan, Ming; Yuan, Lijuan
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human rotavirus (HRV) is the causative agent of severe dehydrating diarrhea in children under the age of five, resulting in up to 215,000 deaths each year. These deaths almost exclusively occur in low- and middle-income countries where vaccine efficacy is the lowest due to chronic malnutrition, gut dysbiosis, and concurrent enteric viral infection. Parenteral vaccines for HRV are particularly attractive as they avoid many of the concerns associated with currently used live oral vaccines. In this study, a two-dose intramuscular (IM) regimen of the trivalent, nanoparticle- based, nonreplicating HRV vaccine (trivalent S60-VP8*), utilizing the shell (S) domain of the capsid of norovirus as an HRV VP8* antigen display platform, was evaluated for immunogenicity and protective efficacy against P[6] and P[8] HRV using gnotobiotic pig models. A prime–boost strategy using one dose of the oral Rotarix® vaccine, followed by one dose of the IM trivalent nanoparticle vaccine was also evaluated. Both regimens were highly immunogenic in inducing serum virus neutralizing, IgG, and IgA antibodies. The two vaccine regimens failed to confer significant protection against diarrhea; however, the prime–boost regimen significantly shortened the duration of virus shedding in pigs challenged orally with the virulent Wa (G1P[8]) HRV and significantly shortened the mean duration of virus shedding, mean peak titer, and area under the curve of virus shedding after challenge with Arg (G4P[6]) HRV. Prime–boost-vaccinated pigs challenged with P[8] HRV had significantly higher P[8]-specific IgG antibody-secreting cells (ASCs) in the spleen post-challenge. Prime–boost-vaccinated pigs challenged with P[6] HRV had significantly higher numbers of P[6]- and P[8]-specific IgG ASCs in the ileum, as well as significantly higher numbers of P[8]-specific IgA ASCs in the spleen post-challenge. These results suggest the promise of and warrant further investigation into the oral priming and parenteral boosting strategy for future HRV vaccines.
Fil: Hensley, Casey. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Nyblade, Charlotte. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Zhou, Peng. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Ramesh, Ashwin. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Frazier, Annie. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Frazier, Maggie. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Garrison, Sarah. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Fantasia Davis, Ariana. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Cai, Ruiqing. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Huang, Peng Wei. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Xia, Ming. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Tan, Ming. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Materia
rotavirus
gn free pig
priming immunization
vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/252379
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/252379
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus InfectionHensley, CaseyNyblade, CharlotteZhou, PengParreño, Gladys VivianaRamesh, AshwinFrazier, AnnieFrazier, MaggieGarrison, SarahFantasia Davis, ArianaCai, RuiqingHuang, Peng WeiXia, MingTan, MingYuan, Lijuanrotavirusgn free pigpriming immunizationvaccinehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human rotavirus (HRV) is the causative agent of severe dehydrating diarrhea in children under the age of five, resulting in up to 215,000 deaths each year. These deaths almost exclusively occur in low- and middle-income countries where vaccine efficacy is the lowest due to chronic malnutrition, gut dysbiosis, and concurrent enteric viral infection. Parenteral vaccines for HRV are particularly attractive as they avoid many of the concerns associated with currently used live oral vaccines. In this study, a two-dose intramuscular (IM) regimen of the trivalent, nanoparticle- based, nonreplicating HRV vaccine (trivalent S60-VP8*), utilizing the shell (S) domain of the capsid of norovirus as an HRV VP8* antigen display platform, was evaluated for immunogenicity and protective efficacy against P[6] and P[8] HRV using gnotobiotic pig models. A prime–boost strategy using one dose of the oral Rotarix® vaccine, followed by one dose of the IM trivalent nanoparticle vaccine was also evaluated. Both regimens were highly immunogenic in inducing serum virus neutralizing, IgG, and IgA antibodies. The two vaccine regimens failed to confer significant protection against diarrhea; however, the prime–boost regimen significantly shortened the duration of virus shedding in pigs challenged orally with the virulent Wa (G1P[8]) HRV and significantly shortened the mean duration of virus shedding, mean peak titer, and area under the curve of virus shedding after challenge with Arg (G4P[6]) HRV. Prime–boost-vaccinated pigs challenged with P[8] HRV had significantly higher P[8]-specific IgG antibody-secreting cells (ASCs) in the spleen post-challenge. Prime–boost-vaccinated pigs challenged with P[6] HRV had significantly higher numbers of P[6]- and P[8]-specific IgG ASCs in the ileum, as well as significantly higher numbers of P[8]-specific IgA ASCs in the spleen post-challenge. These results suggest the promise of and warrant further investigation into the oral priming and parenteral boosting strategy for future HRV vaccines.Fil: Hensley, Casey. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Nyblade, Charlotte. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Zhou, Peng. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; ArgentinaFil: Ramesh, Ashwin. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Frazier, Annie. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Frazier, Maggie. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Garrison, Sarah. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Fantasia Davis, Ariana. Virginia-Maryland College of Veterinary Medicine; Estados UnidosFil: Cai, Ruiqing. Cincinnati Children’s Hospital Medical Center; Estados UnidosFil: Huang, Peng Wei. Cincinnati Children’s Hospital Medical Center; Estados UnidosFil: Xia, Ming. Cincinnati Children’s Hospital Medical Center; Estados UnidosFil: Tan, Ming. Cincinnati Children’s Hospital Medical Center; Estados UnidosFil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados UnidosMDPI2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/252379Hensley, Casey; Nyblade, Charlotte; Zhou, Peng; Parreño, Gladys Viviana; Ramesh, Ashwin; et al.; Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection; MDPI; Vaccines; 11; 5; 5-2023; 1-192076-393XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/11/5/927info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines11050927info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:18Zoai:ri.conicet.gov.ar:11336/252379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:18.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
title Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
spellingShingle Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
Hensley, Casey
rotavirus
gn free pig
priming immunization
vaccine
title_short Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
title_full Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
title_fullStr Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
title_full_unstemmed Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
title_sort Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection
dc.creator.none.fl_str_mv Hensley, Casey
Nyblade, Charlotte
Zhou, Peng
Parreño, Gladys Viviana
Ramesh, Ashwin
Frazier, Annie
Frazier, Maggie
Garrison, Sarah
Fantasia Davis, Ariana
Cai, Ruiqing
Huang, Peng Wei
Xia, Ming
Tan, Ming
Yuan, Lijuan
author Hensley, Casey
author_facet Hensley, Casey
Nyblade, Charlotte
Zhou, Peng
Parreño, Gladys Viviana
Ramesh, Ashwin
Frazier, Annie
Frazier, Maggie
Garrison, Sarah
Fantasia Davis, Ariana
Cai, Ruiqing
Huang, Peng Wei
Xia, Ming
Tan, Ming
Yuan, Lijuan
author_role author
author2 Nyblade, Charlotte
Zhou, Peng
Parreño, Gladys Viviana
Ramesh, Ashwin
Frazier, Annie
Frazier, Maggie
Garrison, Sarah
Fantasia Davis, Ariana
Cai, Ruiqing
Huang, Peng Wei
Xia, Ming
Tan, Ming
Yuan, Lijuan
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv rotavirus
gn free pig
priming immunization
vaccine
topic rotavirus
gn free pig
priming immunization
vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human rotavirus (HRV) is the causative agent of severe dehydrating diarrhea in children under the age of five, resulting in up to 215,000 deaths each year. These deaths almost exclusively occur in low- and middle-income countries where vaccine efficacy is the lowest due to chronic malnutrition, gut dysbiosis, and concurrent enteric viral infection. Parenteral vaccines for HRV are particularly attractive as they avoid many of the concerns associated with currently used live oral vaccines. In this study, a two-dose intramuscular (IM) regimen of the trivalent, nanoparticle- based, nonreplicating HRV vaccine (trivalent S60-VP8*), utilizing the shell (S) domain of the capsid of norovirus as an HRV VP8* antigen display platform, was evaluated for immunogenicity and protective efficacy against P[6] and P[8] HRV using gnotobiotic pig models. A prime–boost strategy using one dose of the oral Rotarix® vaccine, followed by one dose of the IM trivalent nanoparticle vaccine was also evaluated. Both regimens were highly immunogenic in inducing serum virus neutralizing, IgG, and IgA antibodies. The two vaccine regimens failed to confer significant protection against diarrhea; however, the prime–boost regimen significantly shortened the duration of virus shedding in pigs challenged orally with the virulent Wa (G1P[8]) HRV and significantly shortened the mean duration of virus shedding, mean peak titer, and area under the curve of virus shedding after challenge with Arg (G4P[6]) HRV. Prime–boost-vaccinated pigs challenged with P[8] HRV had significantly higher P[8]-specific IgG antibody-secreting cells (ASCs) in the spleen post-challenge. Prime–boost-vaccinated pigs challenged with P[6] HRV had significantly higher numbers of P[6]- and P[8]-specific IgG ASCs in the ileum, as well as significantly higher numbers of P[8]-specific IgA ASCs in the spleen post-challenge. These results suggest the promise of and warrant further investigation into the oral priming and parenteral boosting strategy for future HRV vaccines.
Fil: Hensley, Casey. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Nyblade, Charlotte. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Zhou, Peng. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas. Grupo Vinculado Incuinta Al Ivit.; Argentina
Fil: Ramesh, Ashwin. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Frazier, Annie. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Frazier, Maggie. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Garrison, Sarah. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Fantasia Davis, Ariana. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
Fil: Cai, Ruiqing. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Huang, Peng Wei. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Xia, Ming. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Tan, Ming. Cincinnati Children’s Hospital Medical Center; Estados Unidos
Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine; Estados Unidos
description Human rotavirus (HRV) is the causative agent of severe dehydrating diarrhea in children under the age of five, resulting in up to 215,000 deaths each year. These deaths almost exclusively occur in low- and middle-income countries where vaccine efficacy is the lowest due to chronic malnutrition, gut dysbiosis, and concurrent enteric viral infection. Parenteral vaccines for HRV are particularly attractive as they avoid many of the concerns associated with currently used live oral vaccines. In this study, a two-dose intramuscular (IM) regimen of the trivalent, nanoparticle- based, nonreplicating HRV vaccine (trivalent S60-VP8*), utilizing the shell (S) domain of the capsid of norovirus as an HRV VP8* antigen display platform, was evaluated for immunogenicity and protective efficacy against P[6] and P[8] HRV using gnotobiotic pig models. A prime–boost strategy using one dose of the oral Rotarix® vaccine, followed by one dose of the IM trivalent nanoparticle vaccine was also evaluated. Both regimens were highly immunogenic in inducing serum virus neutralizing, IgG, and IgA antibodies. The two vaccine regimens failed to confer significant protection against diarrhea; however, the prime–boost regimen significantly shortened the duration of virus shedding in pigs challenged orally with the virulent Wa (G1P[8]) HRV and significantly shortened the mean duration of virus shedding, mean peak titer, and area under the curve of virus shedding after challenge with Arg (G4P[6]) HRV. Prime–boost-vaccinated pigs challenged with P[8] HRV had significantly higher P[8]-specific IgG antibody-secreting cells (ASCs) in the spleen post-challenge. Prime–boost-vaccinated pigs challenged with P[6] HRV had significantly higher numbers of P[6]- and P[8]-specific IgG ASCs in the ileum, as well as significantly higher numbers of P[8]-specific IgA ASCs in the spleen post-challenge. These results suggest the promise of and warrant further investigation into the oral priming and parenteral boosting strategy for future HRV vaccines.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/252379
Hensley, Casey; Nyblade, Charlotte; Zhou, Peng; Parreño, Gladys Viviana; Ramesh, Ashwin; et al.; Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection; MDPI; Vaccines; 11; 5; 5-2023; 1-19
2076-393X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/252379
identifier_str_mv Hensley, Casey; Nyblade, Charlotte; Zhou, Peng; Parreño, Gladys Viviana; Ramesh, Ashwin; et al.; Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix® and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection; MDPI; Vaccines; 11; 5; 5-2023; 1-19
2076-393X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/11/5/927
info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines11050927
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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