Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases

Autores
Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The vast majority of Pseudomonas species areunable to fix atmospheric nitrogen. Although severalstudies have demonstrated that some strains belonging tothe genus Pseudomonas sensu stricto do have the ability tofix nitrogen by the expression of horizontally acquirednitrogenase, little is known about the mechanisms ofnitrogenase adaptation to the new bacterial host. Recently,we transferred the nitrogen fixation island from Pseudomonasstutzeri A1501 to the non-nitrogen-fixing bacteriumPseudomonas protegens Pf-5, and interestingly, theresulting recombinant strain Pf-5 X940 showed anuncommon phenotype of constitutive nitrogenase activity.Here, we integrated evolutionary and functional approachesto elucidate this unusual phenotype. Phylogeneticanalysis showed that polyhydroxybutyrate (PHB) biosynthesisgenes from natural nitrogen-fixing Pseudomonasstrains have been acquired by horizontal transfer. Contraryto Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity undernitrogen-excess conditions, and displayed the typicalswitch-on phenotype observed in natural nitrogen-fixingstrains after nitrogen deficiency. This indicates a competitionbetween PHB production and nitrogen fixation.Therefore, we propose that horizontal transfer of PHBbiosynthesis genes could be an ancestral mechanism ofregulation of horizontally acquired nitrogenases in thegenus Pseudomonas.
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Materia
EVOLUTION
HORIZONTAL TRANSFER
NITROGENASE
POLYHYDROXYBUTYRATE
REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113105
Acceder
id CONICETDig_74852132108ed1df1ae8ff30301db559
oai_identifier_str oai:ri.conicet.gov.ar:11336/113105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired NitrogenasesPascuan, Cecilia GabrielaFox, Ana RominaSoto, Gabriela CynthiaAyub, Nicolás DanielEVOLUTIONHORIZONTAL TRANSFERNITROGENASEPOLYHYDROXYBUTYRATEREGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The vast majority of Pseudomonas species areunable to fix atmospheric nitrogen. Although severalstudies have demonstrated that some strains belonging tothe genus Pseudomonas sensu stricto do have the ability tofix nitrogen by the expression of horizontally acquirednitrogenase, little is known about the mechanisms ofnitrogenase adaptation to the new bacterial host. Recently,we transferred the nitrogen fixation island from Pseudomonasstutzeri A1501 to the non-nitrogen-fixing bacteriumPseudomonas protegens Pf-5, and interestingly, theresulting recombinant strain Pf-5 X940 showed anuncommon phenotype of constitutive nitrogenase activity.Here, we integrated evolutionary and functional approachesto elucidate this unusual phenotype. Phylogeneticanalysis showed that polyhydroxybutyrate (PHB) biosynthesisgenes from natural nitrogen-fixing Pseudomonasstrains have been acquired by horizontal transfer. Contraryto Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity undernitrogen-excess conditions, and displayed the typicalswitch-on phenotype observed in natural nitrogen-fixingstrains after nitrogen deficiency. This indicates a competitionbetween PHB production and nitrogen fixation.Therefore, we propose that horizontal transfer of PHBbiosynthesis genes could be an ancestral mechanism ofregulation of horizontally acquired nitrogenases in thegenus Pseudomonas.Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113105Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases; Springer; Journal Of Molecular Evolution; 81; 3-4; 10-2015; 84-890022-2844CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00239-015-9698-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s00239-015-9698-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:16Zoai:ri.conicet.gov.ar:11336/113105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:16.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
title Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
spellingShingle Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
Pascuan, Cecilia Gabriela
EVOLUTION
HORIZONTAL TRANSFER
NITROGENASE
POLYHYDROXYBUTYRATE
REGULATION
title_short Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
title_full Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
title_fullStr Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
title_full_unstemmed Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
title_sort Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases
dc.creator.none.fl_str_mv Pascuan, Cecilia Gabriela
Fox, Ana Romina
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author Pascuan, Cecilia Gabriela
author_facet Pascuan, Cecilia Gabriela
Fox, Ana Romina
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author_role author
author2 Fox, Ana Romina
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv EVOLUTION
HORIZONTAL TRANSFER
NITROGENASE
POLYHYDROXYBUTYRATE
REGULATION
topic EVOLUTION
HORIZONTAL TRANSFER
NITROGENASE
POLYHYDROXYBUTYRATE
REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The vast majority of Pseudomonas species areunable to fix atmospheric nitrogen. Although severalstudies have demonstrated that some strains belonging tothe genus Pseudomonas sensu stricto do have the ability tofix nitrogen by the expression of horizontally acquirednitrogenase, little is known about the mechanisms ofnitrogenase adaptation to the new bacterial host. Recently,we transferred the nitrogen fixation island from Pseudomonasstutzeri A1501 to the non-nitrogen-fixing bacteriumPseudomonas protegens Pf-5, and interestingly, theresulting recombinant strain Pf-5 X940 showed anuncommon phenotype of constitutive nitrogenase activity.Here, we integrated evolutionary and functional approachesto elucidate this unusual phenotype. Phylogeneticanalysis showed that polyhydroxybutyrate (PHB) biosynthesisgenes from natural nitrogen-fixing Pseudomonasstrains have been acquired by horizontal transfer. Contraryto Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity undernitrogen-excess conditions, and displayed the typicalswitch-on phenotype observed in natural nitrogen-fixingstrains after nitrogen deficiency. This indicates a competitionbetween PHB production and nitrogen fixation.Therefore, we propose that horizontal transfer of PHBbiosynthesis genes could be an ancestral mechanism ofregulation of horizontally acquired nitrogenases in thegenus Pseudomonas.
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
description The vast majority of Pseudomonas species areunable to fix atmospheric nitrogen. Although severalstudies have demonstrated that some strains belonging tothe genus Pseudomonas sensu stricto do have the ability tofix nitrogen by the expression of horizontally acquirednitrogenase, little is known about the mechanisms ofnitrogenase adaptation to the new bacterial host. Recently,we transferred the nitrogen fixation island from Pseudomonasstutzeri A1501 to the non-nitrogen-fixing bacteriumPseudomonas protegens Pf-5, and interestingly, theresulting recombinant strain Pf-5 X940 showed anuncommon phenotype of constitutive nitrogenase activity.Here, we integrated evolutionary and functional approachesto elucidate this unusual phenotype. Phylogeneticanalysis showed that polyhydroxybutyrate (PHB) biosynthesisgenes from natural nitrogen-fixing Pseudomonasstrains have been acquired by horizontal transfer. Contraryto Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity undernitrogen-excess conditions, and displayed the typicalswitch-on phenotype observed in natural nitrogen-fixingstrains after nitrogen deficiency. This indicates a competitionbetween PHB production and nitrogen fixation.Therefore, we propose that horizontal transfer of PHBbiosynthesis genes could be an ancestral mechanism ofregulation of horizontally acquired nitrogenases in thegenus Pseudomonas.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113105
Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases; Springer; Journal Of Molecular Evolution; 81; 3-4; 10-2015; 84-89
0022-2844
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113105
identifier_str_mv Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases; Springer; Journal Of Molecular Evolution; 81; 3-4; 10-2015; 84-89
0022-2844
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00239-015-9698-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00239-015-9698-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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