Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens

Autores
Quelas, Juan Ignacio; Mesa, S.; Mongiardini, Elias Javier; Jendrossek, D.; Lodeiro, Anibal
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxia, oxic stationary phase and bacteroids inside legume nodules) were permissive for the synthesis of PHB, which was observed as cytoplasmic granules. To study the regulation of PHB synthesis, we generated mutants in the regulator gene phaR and the phasin genes phaP1 and phaP4. Under permissive conditions, mutation of phaR impaired PHB accumulation, and a phaP1/phaP4 double mutant produced more PHB than the wildtype, which was accumulated in a single, large cytoplasmic granule. Moreover, PhaR negatively regulated the expression of phaP1 and phaP4 as well as of phaA1, phaA2 (3-ketoacyl-CoA thiolase), phaC1, phaC2 (PHB synthases), and fixK2 (CRP/FNR-type transcription regulator of genes for microoxic lifestyle). In addition to the depressed PHB cycling, phaR mutants accumulated more extracellular polysaccharide and promoted higher plant shoot dry weight and competitiveness for nodulation than the wildtype, by contrast to phaC1 mutant strains, defective in PHB synthesis. These results suggest that phaR not only regulates PHB granules formation by controlling the expression of phasins and biosynthetic enzymes, but also acts as global regulator of excess carbon allocation and symbiosis by controlling fixK2.
Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mesa, S.. Consejo Superior de Investigaciones Científicas; España
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Jendrossek, D.. University of Stuttgart; Alemania
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
POLYHYDROXYBUTYRATE
BRADYRHIZOBIUM
SOYBEAN
REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48116
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48116
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiensQuelas, Juan IgnacioMesa, S.Mongiardini, Elias JavierJendrossek, D.Lodeiro, AnibalPOLYHYDROXYBUTYRATEBRADYRHIZOBIUMSOYBEANREGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxia, oxic stationary phase and bacteroids inside legume nodules) were permissive for the synthesis of PHB, which was observed as cytoplasmic granules. To study the regulation of PHB synthesis, we generated mutants in the regulator gene phaR and the phasin genes phaP1 and phaP4. Under permissive conditions, mutation of phaR impaired PHB accumulation, and a phaP1/phaP4 double mutant produced more PHB than the wildtype, which was accumulated in a single, large cytoplasmic granule. Moreover, PhaR negatively regulated the expression of phaP1 and phaP4 as well as of phaA1, phaA2 (3-ketoacyl-CoA thiolase), phaC1, phaC2 (PHB synthases), and fixK2 (CRP/FNR-type transcription regulator of genes for microoxic lifestyle). In addition to the depressed PHB cycling, phaR mutants accumulated more extracellular polysaccharide and promoted higher plant shoot dry weight and competitiveness for nodulation than the wildtype, by contrast to phaC1 mutant strains, defective in PHB synthesis. These results suggest that phaR not only regulates PHB granules formation by controlling the expression of phasins and biosynthetic enzymes, but also acts as global regulator of excess carbon allocation and symbiosis by controlling fixK2.Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Mesa, S.. Consejo Superior de Investigaciones Científicas; EspañaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Jendrossek, D.. University of Stuttgart; AlemaniaFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society for Microbiology2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48116Quelas, Juan Ignacio; Mesa, S.; Mongiardini, Elias Javier; Jendrossek, D.; Lodeiro, Anibal; Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens; American Society for Microbiology; Applied And Environmental Microbiology; 82; 14; 7-2016; 4299-43080099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00757-16info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/14/4299info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:59Zoai:ri.conicet.gov.ar:11336/48116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:59.621CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
title Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
spellingShingle Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
Quelas, Juan Ignacio
POLYHYDROXYBUTYRATE
BRADYRHIZOBIUM
SOYBEAN
REGULATION
title_short Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
title_full Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
title_fullStr Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
title_full_unstemmed Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
title_sort Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens
dc.creator.none.fl_str_mv Quelas, Juan Ignacio
Mesa, S.
Mongiardini, Elias Javier
Jendrossek, D.
Lodeiro, Anibal
author Quelas, Juan Ignacio
author_facet Quelas, Juan Ignacio
Mesa, S.
Mongiardini, Elias Javier
Jendrossek, D.
Lodeiro, Anibal
author_role author
author2 Mesa, S.
Mongiardini, Elias Javier
Jendrossek, D.
Lodeiro, Anibal
author2_role author
author
author
author
dc.subject.none.fl_str_mv POLYHYDROXYBUTYRATE
BRADYRHIZOBIUM
SOYBEAN
REGULATION
topic POLYHYDROXYBUTYRATE
BRADYRHIZOBIUM
SOYBEAN
REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxia, oxic stationary phase and bacteroids inside legume nodules) were permissive for the synthesis of PHB, which was observed as cytoplasmic granules. To study the regulation of PHB synthesis, we generated mutants in the regulator gene phaR and the phasin genes phaP1 and phaP4. Under permissive conditions, mutation of phaR impaired PHB accumulation, and a phaP1/phaP4 double mutant produced more PHB than the wildtype, which was accumulated in a single, large cytoplasmic granule. Moreover, PhaR negatively regulated the expression of phaP1 and phaP4 as well as of phaA1, phaA2 (3-ketoacyl-CoA thiolase), phaC1, phaC2 (PHB synthases), and fixK2 (CRP/FNR-type transcription regulator of genes for microoxic lifestyle). In addition to the depressed PHB cycling, phaR mutants accumulated more extracellular polysaccharide and promoted higher plant shoot dry weight and competitiveness for nodulation than the wildtype, by contrast to phaC1 mutant strains, defective in PHB synthesis. These results suggest that phaR not only regulates PHB granules formation by controlling the expression of phasins and biosynthetic enzymes, but also acts as global regulator of excess carbon allocation and symbiosis by controlling fixK2.
Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mesa, S.. Consejo Superior de Investigaciones Científicas; España
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Jendrossek, D.. University of Stuttgart; Alemania
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxia, oxic stationary phase and bacteroids inside legume nodules) were permissive for the synthesis of PHB, which was observed as cytoplasmic granules. To study the regulation of PHB synthesis, we generated mutants in the regulator gene phaR and the phasin genes phaP1 and phaP4. Under permissive conditions, mutation of phaR impaired PHB accumulation, and a phaP1/phaP4 double mutant produced more PHB than the wildtype, which was accumulated in a single, large cytoplasmic granule. Moreover, PhaR negatively regulated the expression of phaP1 and phaP4 as well as of phaA1, phaA2 (3-ketoacyl-CoA thiolase), phaC1, phaC2 (PHB synthases), and fixK2 (CRP/FNR-type transcription regulator of genes for microoxic lifestyle). In addition to the depressed PHB cycling, phaR mutants accumulated more extracellular polysaccharide and promoted higher plant shoot dry weight and competitiveness for nodulation than the wildtype, by contrast to phaC1 mutant strains, defective in PHB synthesis. These results suggest that phaR not only regulates PHB granules formation by controlling the expression of phasins and biosynthetic enzymes, but also acts as global regulator of excess carbon allocation and symbiosis by controlling fixK2.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48116
Quelas, Juan Ignacio; Mesa, S.; Mongiardini, Elias Javier; Jendrossek, D.; Lodeiro, Anibal; Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens; American Society for Microbiology; Applied And Environmental Microbiology; 82; 14; 7-2016; 4299-4308
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48116
identifier_str_mv Quelas, Juan Ignacio; Mesa, S.; Mongiardini, Elias Javier; Jendrossek, D.; Lodeiro, Anibal; Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens; American Society for Microbiology; Applied And Environmental Microbiology; 82; 14; 7-2016; 4299-4308
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00757-16
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/14/4299
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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