Altered expression of the lymphocyte activation antigen CD30 in active celiac disease

Autores
Periolo, Natalia; Guillen, Laura Cristina; Bernardo, D.; Niveloni, Sonia Isabel; Hwang, H. J.; Garrote, J. A.; BaI, J. C.; Arranz, Silvia Eda; Cherñavsky, Alejandra Claudia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interleukin (IL)-15 and CD30 may be associated with the ongoing intestinal immunologic activation in celiac disease (CD). We studied duodenal biopsies and blood samples of patients with active CD (Cel) and controls in order to determine the regulatory role proposed for CD30þ T cells in this Th1-driven disease and the potential influences of IL-15 on CD30 expression. We detected that a CD30þ T-cell subpopulation persists longer in Cel after a 5 day incubation with anti-CD3 antibody than in controls ( p ¼ 0.0063). CD30 upregulation by IL-15 in T blasts was greater in Cel than in controls ( p ¼ 0.0062). At the mucosal compartment, the CD30 antigen was examined by immunohistochemistry and quantified on isolated lamina propria (LP) and epithelial T cells by flow cytometry. For Cel and controls, similar mean percentages of CD3þCD30þ intraepithelial T cells (5.88 vs. 5.51, p ¼ ns) and LP T cells (7.38 vs. 7.49, p ¼ ns) were observed at baseline and after in vitro gliadin challenge of duodenal biopsy samples. Our study demonstrates the occurrence of potentially important alterations of the immune response at the peripheral compartment. Our findings also allow us to speculate that a negative effect of soluble mediators at the mucosal compartment might counteract the latent influence of IL-15 on CD30 expression precluding a more severe course of active CD.
Fil: Periolo, Natalia. Direccion Nacional de Instituto de Investigacion. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Guillen, Laura Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Bernardo, D.. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Niveloni, Sonia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Hwang, H. J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
Fil: Garrote, J. A.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina. Hospital Clínico Universitario; España
Fil: BaI, J. C.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
Fil: Arranz, Silvia Eda. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Materia
ACTIVE CELIAC DISEASE
CD30 ANTIGEN
PERIPHERAL BLOOD
SMALL INTESTINE MUCOSA
INTERLEUKIN-15
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105026
Acceder
id CONICETDig_7477984bd83ba9ce67ed9e51d00528f6
oai_identifier_str oai:ri.conicet.gov.ar:11336/105026
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered expression of the lymphocyte activation antigen CD30 in active celiac diseasePeriolo, NataliaGuillen, Laura CristinaBernardo, D.Niveloni, Sonia IsabelHwang, H. J.Garrote, J. A.BaI, J. C.Arranz, Silvia EdaCherñavsky, Alejandra ClaudiaACTIVE CELIAC DISEASECD30 ANTIGENPERIPHERAL BLOODSMALL INTESTINE MUCOSAINTERLEUKIN-15https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Interleukin (IL)-15 and CD30 may be associated with the ongoing intestinal immunologic activation in celiac disease (CD). We studied duodenal biopsies and blood samples of patients with active CD (Cel) and controls in order to determine the regulatory role proposed for CD30þ T cells in this Th1-driven disease and the potential influences of IL-15 on CD30 expression. We detected that a CD30þ T-cell subpopulation persists longer in Cel after a 5 day incubation with anti-CD3 antibody than in controls ( p ¼ 0.0063). CD30 upregulation by IL-15 in T blasts was greater in Cel than in controls ( p ¼ 0.0062). At the mucosal compartment, the CD30 antigen was examined by immunohistochemistry and quantified on isolated lamina propria (LP) and epithelial T cells by flow cytometry. For Cel and controls, similar mean percentages of CD3þCD30þ intraepithelial T cells (5.88 vs. 5.51, p ¼ ns) and LP T cells (7.38 vs. 7.49, p ¼ ns) were observed at baseline and after in vitro gliadin challenge of duodenal biopsy samples. Our study demonstrates the occurrence of potentially important alterations of the immune response at the peripheral compartment. Our findings also allow us to speculate that a negative effect of soluble mediators at the mucosal compartment might counteract the latent influence of IL-15 on CD30 expression precluding a more severe course of active CD.Fil: Periolo, Natalia. Direccion Nacional de Instituto de Investigacion. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Guillen, Laura Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Bernardo, D.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Niveloni, Sonia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Hwang, H. J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; ArgentinaFil: Garrote, J. A.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina. Hospital Clínico Universitario; EspañaFil: BaI, J. C.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; ArgentinaFil: Arranz, Silvia Eda. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaTaylor & Francis Ltd2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105026Periolo, Natalia; Guillen, Laura Cristina; Bernardo, D.; Niveloni, Sonia Isabel; Hwang, H. J.; et al.; Altered expression of the lymphocyte activation antigen CD30 in active celiac disease; Taylor & Francis Ltd; Autoimmunity; 43; 4; 2-2010; 288-2980891-6934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/08916930903405867info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/08916930903405867info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-17T14:16:17Zoai:ri.conicet.gov.ar:11336/105026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-17 14:16:17.725CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
title Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
spellingShingle Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
Periolo, Natalia
ACTIVE CELIAC DISEASE
CD30 ANTIGEN
PERIPHERAL BLOOD
SMALL INTESTINE MUCOSA
INTERLEUKIN-15
title_short Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
title_full Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
title_fullStr Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
title_full_unstemmed Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
title_sort Altered expression of the lymphocyte activation antigen CD30 in active celiac disease
dc.creator.none.fl_str_mv Periolo, Natalia
Guillen, Laura Cristina
Bernardo, D.
Niveloni, Sonia Isabel
Hwang, H. J.
Garrote, J. A.
BaI, J. C.
Arranz, Silvia Eda
Cherñavsky, Alejandra Claudia
author Periolo, Natalia
author_facet Periolo, Natalia
Guillen, Laura Cristina
Bernardo, D.
Niveloni, Sonia Isabel
Hwang, H. J.
Garrote, J. A.
BaI, J. C.
Arranz, Silvia Eda
Cherñavsky, Alejandra Claudia
author_role author
author2 Guillen, Laura Cristina
Bernardo, D.
Niveloni, Sonia Isabel
Hwang, H. J.
Garrote, J. A.
BaI, J. C.
Arranz, Silvia Eda
Cherñavsky, Alejandra Claudia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIVE CELIAC DISEASE
CD30 ANTIGEN
PERIPHERAL BLOOD
SMALL INTESTINE MUCOSA
INTERLEUKIN-15
topic ACTIVE CELIAC DISEASE
CD30 ANTIGEN
PERIPHERAL BLOOD
SMALL INTESTINE MUCOSA
INTERLEUKIN-15
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Interleukin (IL)-15 and CD30 may be associated with the ongoing intestinal immunologic activation in celiac disease (CD). We studied duodenal biopsies and blood samples of patients with active CD (Cel) and controls in order to determine the regulatory role proposed for CD30þ T cells in this Th1-driven disease and the potential influences of IL-15 on CD30 expression. We detected that a CD30þ T-cell subpopulation persists longer in Cel after a 5 day incubation with anti-CD3 antibody than in controls ( p ¼ 0.0063). CD30 upregulation by IL-15 in T blasts was greater in Cel than in controls ( p ¼ 0.0062). At the mucosal compartment, the CD30 antigen was examined by immunohistochemistry and quantified on isolated lamina propria (LP) and epithelial T cells by flow cytometry. For Cel and controls, similar mean percentages of CD3þCD30þ intraepithelial T cells (5.88 vs. 5.51, p ¼ ns) and LP T cells (7.38 vs. 7.49, p ¼ ns) were observed at baseline and after in vitro gliadin challenge of duodenal biopsy samples. Our study demonstrates the occurrence of potentially important alterations of the immune response at the peripheral compartment. Our findings also allow us to speculate that a negative effect of soluble mediators at the mucosal compartment might counteract the latent influence of IL-15 on CD30 expression precluding a more severe course of active CD.
Fil: Periolo, Natalia. Direccion Nacional de Instituto de Investigacion. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Guillen, Laura Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Bernardo, D.. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Niveloni, Sonia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Hwang, H. J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
Fil: Garrote, J. A.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina. Hospital Clínico Universitario; España
Fil: BaI, J. C.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
Fil: Arranz, Silvia Eda. Universidad de Valladolid. Facultad de Ciencias; España
Fil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
description Interleukin (IL)-15 and CD30 may be associated with the ongoing intestinal immunologic activation in celiac disease (CD). We studied duodenal biopsies and blood samples of patients with active CD (Cel) and controls in order to determine the regulatory role proposed for CD30þ T cells in this Th1-driven disease and the potential influences of IL-15 on CD30 expression. We detected that a CD30þ T-cell subpopulation persists longer in Cel after a 5 day incubation with anti-CD3 antibody than in controls ( p ¼ 0.0063). CD30 upregulation by IL-15 in T blasts was greater in Cel than in controls ( p ¼ 0.0062). At the mucosal compartment, the CD30 antigen was examined by immunohistochemistry and quantified on isolated lamina propria (LP) and epithelial T cells by flow cytometry. For Cel and controls, similar mean percentages of CD3þCD30þ intraepithelial T cells (5.88 vs. 5.51, p ¼ ns) and LP T cells (7.38 vs. 7.49, p ¼ ns) were observed at baseline and after in vitro gliadin challenge of duodenal biopsy samples. Our study demonstrates the occurrence of potentially important alterations of the immune response at the peripheral compartment. Our findings also allow us to speculate that a negative effect of soluble mediators at the mucosal compartment might counteract the latent influence of IL-15 on CD30 expression precluding a more severe course of active CD.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105026
Periolo, Natalia; Guillen, Laura Cristina; Bernardo, D.; Niveloni, Sonia Isabel; Hwang, H. J.; et al.; Altered expression of the lymphocyte activation antigen CD30 in active celiac disease; Taylor & Francis Ltd; Autoimmunity; 43; 4; 2-2010; 288-298
0891-6934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105026
identifier_str_mv Periolo, Natalia; Guillen, Laura Cristina; Bernardo, D.; Niveloni, Sonia Isabel; Hwang, H. J.; et al.; Altered expression of the lymphocyte activation antigen CD30 in active celiac disease; Taylor & Francis Ltd; Autoimmunity; 43; 4; 2-2010; 288-298
0891-6934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/08916930903405867
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/08916930903405867
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1851851933913972736
score 13.176297

Publicaciones similares