Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease

Autores
Ortega, Hugo Hector; Salvetti, Natalia Raquel; Müller, L. A.; Amable, P.; Lorente, J. A.; Barbeito, Claudio Gustavo; Gimeno, Eduardo Juan
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The distribution of intermediate ¢laments (vimentin, cytokeratins, desmin) and micro¢laments (a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. r
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Müller, L. A.. Universidad Nacional del Litoral; Argentina
Fil: Amable, P.. Universidad Nacional del Litoral; Argentina
Fil: Lorente, J. A.. Universidad Nacional del Litoral; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina
Materia
Cattle
Cysticovary
Follicular cysts
Ovarian disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113585
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113585
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian DiseaseOrtega, Hugo HectorSalvetti, Natalia RaquelMüller, L. A.Amable, P.Lorente, J. A.Barbeito, Claudio GustavoGimeno, Eduardo JuanCattleCysticovaryFollicular cystsOvarian diseasehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The distribution of intermediate ¢laments (vimentin, cytokeratins, desmin) and micro¢laments (a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. rFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Müller, L. A.. Universidad Nacional del Litoral; ArgentinaFil: Amable, P.. Universidad Nacional del Litoral; ArgentinaFil: Lorente, J. A.. Universidad Nacional del Litoral; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; ArgentinaElsevier2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113585Ortega, Hugo Hector; Salvetti, Natalia Raquel; Müller, L. A.; Amable, P.; Lorente, J. A.; et al.; Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease; Elsevier; Journal Of Comparative Pathology; 136; 4; 12-2007; 222-2300021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcpa.2007.02.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:13Zoai:ri.conicet.gov.ar:11336/113585instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:13.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
title Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
spellingShingle Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
Ortega, Hugo Hector
Cattle
Cysticovary
Follicular cysts
Ovarian disease
title_short Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
title_full Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
title_fullStr Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
title_full_unstemmed Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
title_sort Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease
dc.creator.none.fl_str_mv Ortega, Hugo Hector
Salvetti, Natalia Raquel
Müller, L. A.
Amable, P.
Lorente, J. A.
Barbeito, Claudio Gustavo
Gimeno, Eduardo Juan
author Ortega, Hugo Hector
author_facet Ortega, Hugo Hector
Salvetti, Natalia Raquel
Müller, L. A.
Amable, P.
Lorente, J. A.
Barbeito, Claudio Gustavo
Gimeno, Eduardo Juan
author_role author
author2 Salvetti, Natalia Raquel
Müller, L. A.
Amable, P.
Lorente, J. A.
Barbeito, Claudio Gustavo
Gimeno, Eduardo Juan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cattle
Cysticovary
Follicular cysts
Ovarian disease
topic Cattle
Cysticovary
Follicular cysts
Ovarian disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The distribution of intermediate ¢laments (vimentin, cytokeratins, desmin) and micro¢laments (a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. r
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Müller, L. A.. Universidad Nacional del Litoral; Argentina
Fil: Amable, P.. Universidad Nacional del Litoral; Argentina
Fil: Lorente, J. A.. Universidad Nacional del Litoral; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina
description The distribution of intermediate ¢laments (vimentin, cytokeratins, desmin) and micro¢laments (a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. r
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113585
Ortega, Hugo Hector; Salvetti, Natalia Raquel; Müller, L. A.; Amable, P.; Lorente, J. A.; et al.; Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease; Elsevier; Journal Of Comparative Pathology; 136; 4; 12-2007; 222-230
0021-9975
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113585
identifier_str_mv Ortega, Hugo Hector; Salvetti, Natalia Raquel; Müller, L. A.; Amable, P.; Lorente, J. A.; et al.; Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease; Elsevier; Journal Of Comparative Pathology; 136; 4; 12-2007; 222-230
0021-9975
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcpa.2007.02.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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