Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol

Autores
Buekens, Pierre; Cafferata, Maria Luisa; Alger, Jackeline; Althabe, Fernando; Belizan, Jose; Carlier, Yves; Ciganda, Alvaro; Dumonteil, Eric; Gamboa Leon, Rubi; Howard, Elizabeth; Matute, Maria Luisa; Sosa-Estani, Sergio Alejandro; Truyens, Carine; Wesson, Dawn; Zuniga, Concepcion
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Trypanosoma cruzi has been divided into Discrete Typing Units I and non-I (II-VI). T. cruzi I is predominant in Mexico and Central America, while non-I is predominant in most of South America, including Argentina. Little is known about congenital transmission of T. cruzi I. The specific aim of this study is to determine the rate of congenital transmission of T. cruzi I compared to non-I. Methods/design. We are conducting a prospective study to enroll at delivery, 10,000 women in Argentina, 7,500 women in Honduras, and 13,000 women in Mexico. We are measuring transmitted maternal T. cruzi antibodies by performing two rapid tests in cord blood (Stat-Pak, Chembio, Medford, New York, and Trypanosoma Detect, InBios, Seattle, Washington). If at least one of the results is positive, we are identifying infants who are congenitally infected by performing parasitological examinations on cord blood and at 4-8 weeks, and serological follow-up at 10 months. Serological confirmation by ELISA (Wiener, Rosario, Argentina) is performed in cord and maternal blood, and at 10 months. We also are performing T. cruzi standard PCR, real-time quantitative PCR and genotyping on maternal venous blood and on cord blood, and serological examinations on siblings. Data are managed by a Data Center in Montevideo, Uruguay. Data are entered online at the sites in an OpenClinica data management system, and digital pictures of data forms are sent to the Data Center for quality control. Weekly reports allow for rapid feedback to the sites. Trial registration. Observational study with ClinicalTrials.gov Identifier NCT01787968.
Fil: Buekens, Pierre. Tulane University; Estados Unidos
Fil: Cafferata, Maria Luisa. Investigación Clínica y Epidemiológica Montevideo; Uruguay
Fil: Alger, Jackeline. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Honduras
Fil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina
Fil: Belizan, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina
Fil: Carlier, Yves. Université Libre de Bruxelles; Bélgica. Tulane University; Estados Unidos
Fil: Ciganda, Alvaro. Investigación Clínica y Epidemiológica Montevideo; Uruguay
Fil: Dumonteil, Eric. Universidad Autónoma de Yucatán; México
Fil: Gamboa Leon, Rubi. Universidad Autónoma de Yucatán; México
Fil: Howard, Elizabeth. Tulane University; Estados Unidos
Fil: Matute, Maria Luisa. Ministry of Health; Honduras
Fil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Truyens, Carine. Université Libre de Bruxelles; Bélgica
Fil: Wesson, Dawn. Tulane University; Estados Unidos
Fil: Zuniga, Concepcion. Ministry of Health; Honduras. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Honduras
Materia
CHAGAS DISEASE
CONGENITAL TRANSMISSION
LATIN AMERICA
TRYPANOSOMA CRUZI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89497
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/89497
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocolBuekens, PierreCafferata, Maria LuisaAlger, JackelineAlthabe, FernandoBelizan, JoseCarlier, YvesCiganda, AlvaroDumonteil, EricGamboa Leon, RubiHoward, ElizabethMatute, Maria LuisaSosa-Estani, Sergio AlejandroTruyens, CarineWesson, DawnZuniga, ConcepcionCHAGAS DISEASECONGENITAL TRANSMISSIONLATIN AMERICATRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Trypanosoma cruzi has been divided into Discrete Typing Units I and non-I (II-VI). T. cruzi I is predominant in Mexico and Central America, while non-I is predominant in most of South America, including Argentina. Little is known about congenital transmission of T. cruzi I. The specific aim of this study is to determine the rate of congenital transmission of T. cruzi I compared to non-I. Methods/design. We are conducting a prospective study to enroll at delivery, 10,000 women in Argentina, 7,500 women in Honduras, and 13,000 women in Mexico. We are measuring transmitted maternal T. cruzi antibodies by performing two rapid tests in cord blood (Stat-Pak, Chembio, Medford, New York, and Trypanosoma Detect, InBios, Seattle, Washington). If at least one of the results is positive, we are identifying infants who are congenitally infected by performing parasitological examinations on cord blood and at 4-8 weeks, and serological follow-up at 10 months. Serological confirmation by ELISA (Wiener, Rosario, Argentina) is performed in cord and maternal blood, and at 10 months. We also are performing T. cruzi standard PCR, real-time quantitative PCR and genotyping on maternal venous blood and on cord blood, and serological examinations on siblings. Data are managed by a Data Center in Montevideo, Uruguay. Data are entered online at the sites in an OpenClinica data management system, and digital pictures of data forms are sent to the Data Center for quality control. Weekly reports allow for rapid feedback to the sites. Trial registration. Observational study with ClinicalTrials.gov Identifier NCT01787968.Fil: Buekens, Pierre. Tulane University; Estados UnidosFil: Cafferata, Maria Luisa. Investigación Clínica y Epidemiológica Montevideo; UruguayFil: Alger, Jackeline. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; HondurasFil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Belizan, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Carlier, Yves. Université Libre de Bruxelles; Bélgica. Tulane University; Estados UnidosFil: Ciganda, Alvaro. Investigación Clínica y Epidemiológica Montevideo; UruguayFil: Dumonteil, Eric. Universidad Autónoma de Yucatán; MéxicoFil: Gamboa Leon, Rubi. Universidad Autónoma de Yucatán; MéxicoFil: Howard, Elizabeth. Tulane University; Estados UnidosFil: Matute, Maria Luisa. Ministry of Health; HondurasFil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Truyens, Carine. Université Libre de Bruxelles; BélgicaFil: Wesson, Dawn. Tulane University; Estados UnidosFil: Zuniga, Concepcion. Ministry of Health; Honduras. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; HondurasSpringer2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89497Buekens, Pierre; Cafferata, Maria Luisa; Alger, Jackeline; Althabe, Fernando; Belizan, Jose; et al.; Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol; Springer; Reproductive Health; 10; 1; 10-2013; 1-100968-80801742-4755CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://reproductive-health-journal.biomedcentral.com/articles/10.1186/1742-4755-10-55info:eu-repo/semantics/altIdentifier/doi/10.1186/1742-4755-10-55info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852796/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:28Zoai:ri.conicet.gov.ar:11336/89497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:28.564CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
title Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
spellingShingle Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
Buekens, Pierre
CHAGAS DISEASE
CONGENITAL TRANSMISSION
LATIN AMERICA
TRYPANOSOMA CRUZI
title_short Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
title_full Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
title_fullStr Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
title_full_unstemmed Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
title_sort Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol
dc.creator.none.fl_str_mv Buekens, Pierre
Cafferata, Maria Luisa
Alger, Jackeline
Althabe, Fernando
Belizan, Jose
Carlier, Yves
Ciganda, Alvaro
Dumonteil, Eric
Gamboa Leon, Rubi
Howard, Elizabeth
Matute, Maria Luisa
Sosa-Estani, Sergio Alejandro
Truyens, Carine
Wesson, Dawn
Zuniga, Concepcion
author Buekens, Pierre
author_facet Buekens, Pierre
Cafferata, Maria Luisa
Alger, Jackeline
Althabe, Fernando
Belizan, Jose
Carlier, Yves
Ciganda, Alvaro
Dumonteil, Eric
Gamboa Leon, Rubi
Howard, Elizabeth
Matute, Maria Luisa
Sosa-Estani, Sergio Alejandro
Truyens, Carine
Wesson, Dawn
Zuniga, Concepcion
author_role author
author2 Cafferata, Maria Luisa
Alger, Jackeline
Althabe, Fernando
Belizan, Jose
Carlier, Yves
Ciganda, Alvaro
Dumonteil, Eric
Gamboa Leon, Rubi
Howard, Elizabeth
Matute, Maria Luisa
Sosa-Estani, Sergio Alejandro
Truyens, Carine
Wesson, Dawn
Zuniga, Concepcion
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
CONGENITAL TRANSMISSION
LATIN AMERICA
TRYPANOSOMA CRUZI
topic CHAGAS DISEASE
CONGENITAL TRANSMISSION
LATIN AMERICA
TRYPANOSOMA CRUZI
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Trypanosoma cruzi has been divided into Discrete Typing Units I and non-I (II-VI). T. cruzi I is predominant in Mexico and Central America, while non-I is predominant in most of South America, including Argentina. Little is known about congenital transmission of T. cruzi I. The specific aim of this study is to determine the rate of congenital transmission of T. cruzi I compared to non-I. Methods/design. We are conducting a prospective study to enroll at delivery, 10,000 women in Argentina, 7,500 women in Honduras, and 13,000 women in Mexico. We are measuring transmitted maternal T. cruzi antibodies by performing two rapid tests in cord blood (Stat-Pak, Chembio, Medford, New York, and Trypanosoma Detect, InBios, Seattle, Washington). If at least one of the results is positive, we are identifying infants who are congenitally infected by performing parasitological examinations on cord blood and at 4-8 weeks, and serological follow-up at 10 months. Serological confirmation by ELISA (Wiener, Rosario, Argentina) is performed in cord and maternal blood, and at 10 months. We also are performing T. cruzi standard PCR, real-time quantitative PCR and genotyping on maternal venous blood and on cord blood, and serological examinations on siblings. Data are managed by a Data Center in Montevideo, Uruguay. Data are entered online at the sites in an OpenClinica data management system, and digital pictures of data forms are sent to the Data Center for quality control. Weekly reports allow for rapid feedback to the sites. Trial registration. Observational study with ClinicalTrials.gov Identifier NCT01787968.
Fil: Buekens, Pierre. Tulane University; Estados Unidos
Fil: Cafferata, Maria Luisa. Investigación Clínica y Epidemiológica Montevideo; Uruguay
Fil: Alger, Jackeline. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Honduras
Fil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina
Fil: Belizan, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina
Fil: Carlier, Yves. Université Libre de Bruxelles; Bélgica. Tulane University; Estados Unidos
Fil: Ciganda, Alvaro. Investigación Clínica y Epidemiológica Montevideo; Uruguay
Fil: Dumonteil, Eric. Universidad Autónoma de Yucatán; México
Fil: Gamboa Leon, Rubi. Universidad Autónoma de Yucatán; México
Fil: Howard, Elizabeth. Tulane University; Estados Unidos
Fil: Matute, Maria Luisa. Ministry of Health; Honduras
Fil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Truyens, Carine. Université Libre de Bruxelles; Bélgica
Fil: Wesson, Dawn. Tulane University; Estados Unidos
Fil: Zuniga, Concepcion. Ministry of Health; Honduras. Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Honduras
description Background: Trypanosoma cruzi has been divided into Discrete Typing Units I and non-I (II-VI). T. cruzi I is predominant in Mexico and Central America, while non-I is predominant in most of South America, including Argentina. Little is known about congenital transmission of T. cruzi I. The specific aim of this study is to determine the rate of congenital transmission of T. cruzi I compared to non-I. Methods/design. We are conducting a prospective study to enroll at delivery, 10,000 women in Argentina, 7,500 women in Honduras, and 13,000 women in Mexico. We are measuring transmitted maternal T. cruzi antibodies by performing two rapid tests in cord blood (Stat-Pak, Chembio, Medford, New York, and Trypanosoma Detect, InBios, Seattle, Washington). If at least one of the results is positive, we are identifying infants who are congenitally infected by performing parasitological examinations on cord blood and at 4-8 weeks, and serological follow-up at 10 months. Serological confirmation by ELISA (Wiener, Rosario, Argentina) is performed in cord and maternal blood, and at 10 months. We also are performing T. cruzi standard PCR, real-time quantitative PCR and genotyping on maternal venous blood and on cord blood, and serological examinations on siblings. Data are managed by a Data Center in Montevideo, Uruguay. Data are entered online at the sites in an OpenClinica data management system, and digital pictures of data forms are sent to the Data Center for quality control. Weekly reports allow for rapid feedback to the sites. Trial registration. Observational study with ClinicalTrials.gov Identifier NCT01787968.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89497
Buekens, Pierre; Cafferata, Maria Luisa; Alger, Jackeline; Althabe, Fernando; Belizan, Jose; et al.; Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol; Springer; Reproductive Health; 10; 1; 10-2013; 1-10
0968-8080
1742-4755
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89497
identifier_str_mv Buekens, Pierre; Cafferata, Maria Luisa; Alger, Jackeline; Althabe, Fernando; Belizan, Jose; et al.; Congenital transmission of Trypanosoma cruzi in Argentina, Honduras, and Mexico: Study protocol; Springer; Reproductive Health; 10; 1; 10-2013; 1-10
0968-8080
1742-4755
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/1742-4755-10-55
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852796/
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dc.publisher.none.fl_str_mv Springer
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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