Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis

Autores
Diniz Luerce, Tessália; Gomes Santos, Ana Cristina; Santos Rochat, Clarissa; Garcias Moreira, Thais; Nogueira Cruz, Déborah; Lemos, Luísa; Sousa, Adna Luciana; Bastos Pereira, Vanessa; de Azevedo, Marcela; Moraes, Kátia; Carmona Cara, Denise; Leblanc, Jean Guy Joseph; Azevedo, Vasco; Caetano Faria, Ana Maria; Miyoshi, Anderson
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most of these bacteria are lactic acid bacteria, which are part of the healthy human microbiota. However, little is known about the effects of transient bacteria present in normal diets, including Lactococcus lactis. Methods. In the present study, we analysed the immunomodulatory effects of three L. lactis strains in vitro using intestinal epithelial cells. L. lactis NCDO 2118 was administered for 4 days to C57BL/6 mice during the remission period of colitis induced by dextran sodium sulphate (DSS). Results: Only one strain, L. lactis NCDO 2118, was able to reduce IL-1β-induced IL-8 secretion in Caco-2 cells, suggesting a potential anti-inflammatory effect. Oral treatment using L. lactis NCDO 2118 resulted in a milder form of recurrent colitis than that observed in control diseased mice. This protective effect was not attributable to changes in secretory IgA (sIgA); however, NCDO 2118 administration was associated with an early increase in IL-6 production and sustained IL-10 production in colonic tissue. Mice fed L. lactis NCDO 2118 had an increased number of regulatory CD4+T cells (Tregs) bearing surface TGF-β in its latent form (Latency-associated peptide-LAP) in the mesenteric lymph nodes and spleen. Conclusions: Here, we identified a new probiotic strain with a potential role in the treatment of IBD, and we elucidated some of the mechanisms underlying its anti-inflammatory effect.
Fil: Diniz Luerce, Tessália. Universidade Federal de Minas Gerais; Brasil
Fil: Gomes Santos, Ana Cristina. Universidade Federal de Minas Gerais; Brasil
Fil: Santos Rochat, Clarissa. Universidade Federal de Minas Gerais; Brasil
Fil: Garcias Moreira, Thais. Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Ciência de Alimentos.; Brasil
Fil: Nogueira Cruz, Déborah. Universidade Federal de Minas Gerais; Brasil
Fil: Lemos, Luísa. Universidade Federal de Minas Gerais; Brasil
Fil: Sousa, Adna Luciana. Universidade Federal de Minas Gerais; Brasil
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; Brasil
Fil: Moraes, Kátia. Universidade Federal de Minas Gerais; Brasil
Fil: Carmona Cara, Denise. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Caetano Faria, Ana Maria. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
Materia
COLITIS
CYTOKINES
LACTOCOCCUS LACTIS
PROBIOTICS
REGULATORY T CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2903
Acceder
id CONICETDig_73c4f62af7cf2142886035063c0b957a
oai_identifier_str oai:ri.conicet.gov.ar:11336/2903
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitisDiniz Luerce, TessáliaGomes Santos, Ana CristinaSantos Rochat, ClarissaGarcias Moreira, ThaisNogueira Cruz, DéborahLemos, LuísaSousa, Adna LucianaBastos Pereira, Vanessade Azevedo, MarcelaMoraes, KátiaCarmona Cara, DeniseLeblanc, Jean Guy JosephAzevedo, VascoCaetano Faria, Ana MariaMiyoshi, AndersonCOLITISCYTOKINESLACTOCOCCUS LACTISPROBIOTICSREGULATORY T CELLShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Background: Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most of these bacteria are lactic acid bacteria, which are part of the healthy human microbiota. However, little is known about the effects of transient bacteria present in normal diets, including Lactococcus lactis. Methods. In the present study, we analysed the immunomodulatory effects of three L. lactis strains in vitro using intestinal epithelial cells. L. lactis NCDO 2118 was administered for 4 days to C57BL/6 mice during the remission period of colitis induced by dextran sodium sulphate (DSS). Results: Only one strain, L. lactis NCDO 2118, was able to reduce IL-1β-induced IL-8 secretion in Caco-2 cells, suggesting a potential anti-inflammatory effect. Oral treatment using L. lactis NCDO 2118 resulted in a milder form of recurrent colitis than that observed in control diseased mice. This protective effect was not attributable to changes in secretory IgA (sIgA); however, NCDO 2118 administration was associated with an early increase in IL-6 production and sustained IL-10 production in colonic tissue. Mice fed L. lactis NCDO 2118 had an increased number of regulatory CD4+T cells (Tregs) bearing surface TGF-β in its latent form (Latency-associated peptide-LAP) in the mesenteric lymph nodes and spleen. Conclusions: Here, we identified a new probiotic strain with a potential role in the treatment of IBD, and we elucidated some of the mechanisms underlying its anti-inflammatory effect.Fil: Diniz Luerce, Tessália. Universidade Federal de Minas Gerais; BrasilFil: Gomes Santos, Ana Cristina. Universidade Federal de Minas Gerais; BrasilFil: Santos Rochat, Clarissa. Universidade Federal de Minas Gerais; BrasilFil: Garcias Moreira, Thais. Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Ciência de Alimentos.; BrasilFil: Nogueira Cruz, Déborah. Universidade Federal de Minas Gerais; BrasilFil: Lemos, Luísa. Universidade Federal de Minas Gerais; BrasilFil: Sousa, Adna Luciana. Universidade Federal de Minas Gerais; BrasilFil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; BrasilFil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; BrasilFil: Moraes, Kátia. Universidade Federal de Minas Gerais; BrasilFil: Carmona Cara, Denise. Universidade Federal de Minas Gerais; BrasilFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Azevedo, Vasco. Universidade Federal de Minas Gerais; BrasilFil: Caetano Faria, Ana Maria. Universidade Federal de Minas Gerais; BrasilFil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; BrasilBioMed Central2014-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2903Diniz Luerce, Tessália; Gomes Santos, Ana Cristina; Santos Rochat, Clarissa; Garcias Moreira, Thais; Nogueira Cruz, Déborah; et al.; Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis; BioMed Central; Gut Pathogens; 6; 33; 29-7-2014; 1-111757-47491757-4749enginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1757-4749-6-33info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:28Zoai:ri.conicet.gov.ar:11336/2903instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:28.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
title Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
spellingShingle Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
Diniz Luerce, Tessália
COLITIS
CYTOKINES
LACTOCOCCUS LACTIS
PROBIOTICS
REGULATORY T CELLS
title_short Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
title_full Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
title_fullStr Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
title_full_unstemmed Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
title_sort Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis
dc.creator.none.fl_str_mv Diniz Luerce, Tessália
Gomes Santos, Ana Cristina
Santos Rochat, Clarissa
Garcias Moreira, Thais
Nogueira Cruz, Déborah
Lemos, Luísa
Sousa, Adna Luciana
Bastos Pereira, Vanessa
de Azevedo, Marcela
Moraes, Kátia
Carmona Cara, Denise
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Caetano Faria, Ana Maria
Miyoshi, Anderson
author Diniz Luerce, Tessália
author_facet Diniz Luerce, Tessália
Gomes Santos, Ana Cristina
Santos Rochat, Clarissa
Garcias Moreira, Thais
Nogueira Cruz, Déborah
Lemos, Luísa
Sousa, Adna Luciana
Bastos Pereira, Vanessa
de Azevedo, Marcela
Moraes, Kátia
Carmona Cara, Denise
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Caetano Faria, Ana Maria
Miyoshi, Anderson
author_role author
author2 Gomes Santos, Ana Cristina
Santos Rochat, Clarissa
Garcias Moreira, Thais
Nogueira Cruz, Déborah
Lemos, Luísa
Sousa, Adna Luciana
Bastos Pereira, Vanessa
de Azevedo, Marcela
Moraes, Kátia
Carmona Cara, Denise
Leblanc, Jean Guy Joseph
Azevedo, Vasco
Caetano Faria, Ana Maria
Miyoshi, Anderson
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COLITIS
CYTOKINES
LACTOCOCCUS LACTIS
PROBIOTICS
REGULATORY T CELLS
topic COLITIS
CYTOKINES
LACTOCOCCUS LACTIS
PROBIOTICS
REGULATORY T CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most of these bacteria are lactic acid bacteria, which are part of the healthy human microbiota. However, little is known about the effects of transient bacteria present in normal diets, including Lactococcus lactis. Methods. In the present study, we analysed the immunomodulatory effects of three L. lactis strains in vitro using intestinal epithelial cells. L. lactis NCDO 2118 was administered for 4 days to C57BL/6 mice during the remission period of colitis induced by dextran sodium sulphate (DSS). Results: Only one strain, L. lactis NCDO 2118, was able to reduce IL-1β-induced IL-8 secretion in Caco-2 cells, suggesting a potential anti-inflammatory effect. Oral treatment using L. lactis NCDO 2118 resulted in a milder form of recurrent colitis than that observed in control diseased mice. This protective effect was not attributable to changes in secretory IgA (sIgA); however, NCDO 2118 administration was associated with an early increase in IL-6 production and sustained IL-10 production in colonic tissue. Mice fed L. lactis NCDO 2118 had an increased number of regulatory CD4+T cells (Tregs) bearing surface TGF-β in its latent form (Latency-associated peptide-LAP) in the mesenteric lymph nodes and spleen. Conclusions: Here, we identified a new probiotic strain with a potential role in the treatment of IBD, and we elucidated some of the mechanisms underlying its anti-inflammatory effect.
Fil: Diniz Luerce, Tessália. Universidade Federal de Minas Gerais; Brasil
Fil: Gomes Santos, Ana Cristina. Universidade Federal de Minas Gerais; Brasil
Fil: Santos Rochat, Clarissa. Universidade Federal de Minas Gerais; Brasil
Fil: Garcias Moreira, Thais. Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Ciência de Alimentos.; Brasil
Fil: Nogueira Cruz, Déborah. Universidade Federal de Minas Gerais; Brasil
Fil: Lemos, Luísa. Universidade Federal de Minas Gerais; Brasil
Fil: Sousa, Adna Luciana. Universidade Federal de Minas Gerais; Brasil
Fil: Bastos Pereira, Vanessa. Universidade Federal de Minas Gerais; Brasil
Fil: de Azevedo, Marcela. Universidade Federal de Minas Gerais; Brasil
Fil: Moraes, Kátia. Universidade Federal de Minas Gerais; Brasil
Fil: Carmona Cara, Denise. Universidade Federal de Minas Gerais; Brasil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil
Fil: Caetano Faria, Ana Maria. Universidade Federal de Minas Gerais; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais; Brasil
description Background: Many probiotic bacteria have been described as promising tools for the treatment and prevention of inflammatory bowel diseases (IBDs). Most of these bacteria are lactic acid bacteria, which are part of the healthy human microbiota. However, little is known about the effects of transient bacteria present in normal diets, including Lactococcus lactis. Methods. In the present study, we analysed the immunomodulatory effects of three L. lactis strains in vitro using intestinal epithelial cells. L. lactis NCDO 2118 was administered for 4 days to C57BL/6 mice during the remission period of colitis induced by dextran sodium sulphate (DSS). Results: Only one strain, L. lactis NCDO 2118, was able to reduce IL-1β-induced IL-8 secretion in Caco-2 cells, suggesting a potential anti-inflammatory effect. Oral treatment using L. lactis NCDO 2118 resulted in a milder form of recurrent colitis than that observed in control diseased mice. This protective effect was not attributable to changes in secretory IgA (sIgA); however, NCDO 2118 administration was associated with an early increase in IL-6 production and sustained IL-10 production in colonic tissue. Mice fed L. lactis NCDO 2118 had an increased number of regulatory CD4+T cells (Tregs) bearing surface TGF-β in its latent form (Latency-associated peptide-LAP) in the mesenteric lymph nodes and spleen. Conclusions: Here, we identified a new probiotic strain with a potential role in the treatment of IBD, and we elucidated some of the mechanisms underlying its anti-inflammatory effect.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2903
Diniz Luerce, Tessália; Gomes Santos, Ana Cristina; Santos Rochat, Clarissa; Garcias Moreira, Thais; Nogueira Cruz, Déborah; et al.; Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis; BioMed Central; Gut Pathogens; 6; 33; 29-7-2014; 1-11
1757-4749
1757-4749
url http://hdl.handle.net/11336/2903
identifier_str_mv Diniz Luerce, Tessália; Gomes Santos, Ana Cristina; Santos Rochat, Clarissa; Garcias Moreira, Thais; Nogueira Cruz, Déborah; et al.; Anti-inflammatory effects of Lactococcus lactis NCDO 2118 during the remission period of chemically-induced colitis; BioMed Central; Gut Pathogens; 6; 33; 29-7-2014; 1-11
1757-4749
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1757-4749-6-33
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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