Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation

Autores
Marques Da Silva, Wanderson; Castro Oliveira, Leticia; Castro Soares, Siomar; Severiano Sousa, Cassiana; Campos Tavares, Guilherme; Perdigão Resende, Cristiana; Pereira, Felipe Luis; Ghosh, Preetam; Figueiredo, Henrique; Azevedo, Vasco
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactococcus lactis is a gram positive facultative anaerobe widely used in the dairy industry and human health. L. lactis subsp. lactis NCDO 2118 is a strain that exhibits anti-inflammatory and immunomodulatory properties. In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-aerated (30°C and 37°C) conditions, respectively. Our label-free proteomic analysis quantified a total of 1,239 proteins amongst which 161 proteins were statistically differentially expressed. Main differences were observed in cellular metabolism, stress response, transcription and proteins associated to cell wall. In addition, we identified six strain-specific proteins of NCDO 2118. Altogether, the results obtained in our study will help to improve the understanding about the factors related to both physiology and adaptive processes of L. lactis NCDO 2118 under changing environmental conditions.
Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Castro Oliveira, Leticia. Universidade Federal Do Triangulo Mineiro; Brasil
Fil: Castro Soares, Siomar. Universidade Federal Do Triangulo Mineiro; Brasil
Fil: Severiano Sousa, Cassiana. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Campos Tavares, Guilherme. No especifíca;
Fil: Perdigão Resende, Cristiana. No especifíca;
Fil: Pereira, Felipe Luis. No especifíca;
Fil: Ghosh, Preetam. Virginia Commonwealth University; Estados Unidos
Fil: Figueiredo, Henrique. No especifíca;
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Materia
BACTERIAL PHYSIOLOGY
BACTERIAL PROTEOMIC
BACTERIAL STRESS
LABEL-FREE PROTEOMICS
LACTOCOCCUS LACTIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147884
Acceder
id CONICETDig_5b8f25eee8dac615be18a2748710898c
oai_identifier_str oai:ri.conicet.gov.ar:11336/147884
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variationMarques Da Silva, WandersonCastro Oliveira, LeticiaCastro Soares, SiomarSeveriano Sousa, CassianaCampos Tavares, GuilhermePerdigão Resende, CristianaPereira, Felipe LuisGhosh, PreetamFigueiredo, HenriqueAzevedo, VascoBACTERIAL PHYSIOLOGYBACTERIAL PROTEOMICBACTERIAL STRESSLABEL-FREE PROTEOMICSLACTOCOCCUS LACTIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactococcus lactis is a gram positive facultative anaerobe widely used in the dairy industry and human health. L. lactis subsp. lactis NCDO 2118 is a strain that exhibits anti-inflammatory and immunomodulatory properties. In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-aerated (30°C and 37°C) conditions, respectively. Our label-free proteomic analysis quantified a total of 1,239 proteins amongst which 161 proteins were statistically differentially expressed. Main differences were observed in cellular metabolism, stress response, transcription and proteins associated to cell wall. In addition, we identified six strain-specific proteins of NCDO 2118. Altogether, the results obtained in our study will help to improve the understanding about the factors related to both physiology and adaptive processes of L. lactis NCDO 2118 under changing environmental conditions.Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Castro Oliveira, Leticia. Universidade Federal Do Triangulo Mineiro; BrasilFil: Castro Soares, Siomar. Universidade Federal Do Triangulo Mineiro; BrasilFil: Severiano Sousa, Cassiana. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Campos Tavares, Guilherme. No especifíca;Fil: Perdigão Resende, Cristiana. No especifíca;Fil: Pereira, Felipe Luis. No especifíca;Fil: Ghosh, Preetam. Virginia Commonwealth University; Estados UnidosFil: Figueiredo, Henrique. No especifíca;Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFrontiers Media2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147884Marques Da Silva, Wanderson; Castro Oliveira, Leticia; Castro Soares, Siomar; Severiano Sousa, Cassiana; Campos Tavares, Guilherme; et al.; Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation; Frontiers Media; Frontiers in Microbiology; 10; APR; 4-2019; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00759/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00759info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:05Zoai:ri.conicet.gov.ar:11336/147884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:05.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
title Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
spellingShingle Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
Marques Da Silva, Wanderson
BACTERIAL PHYSIOLOGY
BACTERIAL PROTEOMIC
BACTERIAL STRESS
LABEL-FREE PROTEOMICS
LACTOCOCCUS LACTIS
title_short Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
title_full Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
title_fullStr Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
title_full_unstemmed Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
title_sort Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation
dc.creator.none.fl_str_mv Marques Da Silva, Wanderson
Castro Oliveira, Leticia
Castro Soares, Siomar
Severiano Sousa, Cassiana
Campos Tavares, Guilherme
Perdigão Resende, Cristiana
Pereira, Felipe Luis
Ghosh, Preetam
Figueiredo, Henrique
Azevedo, Vasco
author Marques Da Silva, Wanderson
author_facet Marques Da Silva, Wanderson
Castro Oliveira, Leticia
Castro Soares, Siomar
Severiano Sousa, Cassiana
Campos Tavares, Guilherme
Perdigão Resende, Cristiana
Pereira, Felipe Luis
Ghosh, Preetam
Figueiredo, Henrique
Azevedo, Vasco
author_role author
author2 Castro Oliveira, Leticia
Castro Soares, Siomar
Severiano Sousa, Cassiana
Campos Tavares, Guilherme
Perdigão Resende, Cristiana
Pereira, Felipe Luis
Ghosh, Preetam
Figueiredo, Henrique
Azevedo, Vasco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIAL PHYSIOLOGY
BACTERIAL PROTEOMIC
BACTERIAL STRESS
LABEL-FREE PROTEOMICS
LACTOCOCCUS LACTIS
topic BACTERIAL PHYSIOLOGY
BACTERIAL PROTEOMIC
BACTERIAL STRESS
LABEL-FREE PROTEOMICS
LACTOCOCCUS LACTIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactococcus lactis is a gram positive facultative anaerobe widely used in the dairy industry and human health. L. lactis subsp. lactis NCDO 2118 is a strain that exhibits anti-inflammatory and immunomodulatory properties. In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-aerated (30°C and 37°C) conditions, respectively. Our label-free proteomic analysis quantified a total of 1,239 proteins amongst which 161 proteins were statistically differentially expressed. Main differences were observed in cellular metabolism, stress response, transcription and proteins associated to cell wall. In addition, we identified six strain-specific proteins of NCDO 2118. Altogether, the results obtained in our study will help to improve the understanding about the factors related to both physiology and adaptive processes of L. lactis NCDO 2118 under changing environmental conditions.
Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Castro Oliveira, Leticia. Universidade Federal Do Triangulo Mineiro; Brasil
Fil: Castro Soares, Siomar. Universidade Federal Do Triangulo Mineiro; Brasil
Fil: Severiano Sousa, Cassiana. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Campos Tavares, Guilherme. No especifíca;
Fil: Perdigão Resende, Cristiana. No especifíca;
Fil: Pereira, Felipe Luis. No especifíca;
Fil: Ghosh, Preetam. Virginia Commonwealth University; Estados Unidos
Fil: Figueiredo, Henrique. No especifíca;
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
description Lactococcus lactis is a gram positive facultative anaerobe widely used in the dairy industry and human health. L. lactis subsp. lactis NCDO 2118 is a strain that exhibits anti-inflammatory and immunomodulatory properties. In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-aerated (30°C and 37°C) conditions, respectively. Our label-free proteomic analysis quantified a total of 1,239 proteins amongst which 161 proteins were statistically differentially expressed. Main differences were observed in cellular metabolism, stress response, transcription and proteins associated to cell wall. In addition, we identified six strain-specific proteins of NCDO 2118. Altogether, the results obtained in our study will help to improve the understanding about the factors related to both physiology and adaptive processes of L. lactis NCDO 2118 under changing environmental conditions.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147884
Marques Da Silva, Wanderson; Castro Oliveira, Leticia; Castro Soares, Siomar; Severiano Sousa, Cassiana; Campos Tavares, Guilherme; et al.; Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation; Frontiers Media; Frontiers in Microbiology; 10; APR; 4-2019; 1-14
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147884
identifier_str_mv Marques Da Silva, Wanderson; Castro Oliveira, Leticia; Castro Soares, Siomar; Severiano Sousa, Cassiana; Campos Tavares, Guilherme; et al.; Quantitative proteomic analysis of the response of probiotic putative lactococcus lactis NCDO 2118 strain to different oxygen availability under temperature variation; Frontiers Media; Frontiers in Microbiology; 10; APR; 4-2019; 1-14
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00759/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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