Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved

Autores
Medina, María Fernanda; Pucci, Ana; Pucci, Franco José; Bühler, Marta Ines; Gramajo Bühler, María Cecilia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.
Fil: Medina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pucci, Ana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Pucci, Franco José. Fundación Miguel Lillo; Argentina
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
ACROSOME REACTION
DEHYDROLEUCODINE
EPIDIDYMAL SPERMATOZOA
SESQUITERPENE LACTONES
SPERM QUALITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257133
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/257133
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involvedMedina, María FernandaPucci, AnaPucci, Franco JoséBühler, Marta InesGramajo Bühler, María CeciliaACROSOME REACTIONDEHYDROLEUCODINEEPIDIDYMAL SPERMATOZOASESQUITERPENE LACTONESSPERM QUALITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.Fil: Medina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pucci, Ana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Pucci, Franco José. Fundación Miguel Lillo; ArgentinaFil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaCambridge University Press2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257133Medina, María Fernanda; Pucci, Ana; Pucci, Franco José; Bühler, Marta Ines; Gramajo Bühler, María Cecilia; Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved; Cambridge University Press; Zygote; 32; 6; 11-2024; 446-4510967-1994CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S096719942400042X/type/journal_articleinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S096719942400042Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:47Zoai:ri.conicet.gov.ar:11336/257133instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:47.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
title Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
spellingShingle Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
Medina, María Fernanda
ACROSOME REACTION
DEHYDROLEUCODINE
EPIDIDYMAL SPERMATOZOA
SESQUITERPENE LACTONES
SPERM QUALITY
title_short Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
title_full Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
title_fullStr Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
title_full_unstemmed Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
title_sort Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved
dc.creator.none.fl_str_mv Medina, María Fernanda
Pucci, Ana
Pucci, Franco José
Bühler, Marta Ines
Gramajo Bühler, María Cecilia
author Medina, María Fernanda
author_facet Medina, María Fernanda
Pucci, Ana
Pucci, Franco José
Bühler, Marta Ines
Gramajo Bühler, María Cecilia
author_role author
author2 Pucci, Ana
Pucci, Franco José
Bühler, Marta Ines
Gramajo Bühler, María Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACROSOME REACTION
DEHYDROLEUCODINE
EPIDIDYMAL SPERMATOZOA
SESQUITERPENE LACTONES
SPERM QUALITY
topic ACROSOME REACTION
DEHYDROLEUCODINE
EPIDIDYMAL SPERMATOZOA
SESQUITERPENE LACTONES
SPERM QUALITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.
Fil: Medina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pucci, Ana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Pucci, Franco José. Fundación Miguel Lillo; Argentina
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257133
Medina, María Fernanda; Pucci, Ana; Pucci, Franco José; Bühler, Marta Ines; Gramajo Bühler, María Cecilia; Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved; Cambridge University Press; Zygote; 32; 6; 11-2024; 446-451
0967-1994
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257133
identifier_str_mv Medina, María Fernanda; Pucci, Ana; Pucci, Franco José; Bühler, Marta Ines; Gramajo Bühler, María Cecilia; Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera : signalling pathways involved; Cambridge University Press; Zygote; 32; 6; 11-2024; 446-451
0967-1994
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S096719942400042X/type/journal_article
info:eu-repo/semantics/altIdentifier/doi/10.1017/S096719942400042X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269182762156032
score 13.13397

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