Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera

Autores
Gramajo Bühler, María Cecilia; Pucci Alcaide, F.; Seisdedos de Sánchez Toranzo, Graciela del Valle
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Summary Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen-thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material. Copyright © Cambridge University Press 2012.
Fil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo | Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo; Argentina
Fil: Pucci Alcaide, F.. Fundación Miguel Lillo; Argentina
Fil: Seisdedos de Sánchez Toranzo, Graciela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Chinchilla Lanigera
Cryopreservation
Dna Fragmentation
Epididymal Sperm
Sperm Quality
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62618
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62618
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigeraGramajo Bühler, María CeciliaPucci Alcaide, F.Seisdedos de Sánchez Toranzo, Graciela del ValleChinchilla LanigeraCryopreservationDna FragmentationEpididymal SpermSperm Qualityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Summary Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen-thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material. Copyright © Cambridge University Press 2012.Fil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo | Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo; ArgentinaFil: Pucci Alcaide, F.. Fundación Miguel Lillo; ArgentinaFil: Seisdedos de Sánchez Toranzo, Graciela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaCambridge University Press2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62618Gramajo Bühler, María Cecilia; Pucci Alcaide, F.; Seisdedos de Sánchez Toranzo, Graciela del Valle; Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera; Cambridge University Press; Zygote; 21; 4; 11-2013; 351-3570967-1994CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/effect-on-sperm-quality-of-different-cryoprotectants-in-sperm-of-chinchilla-lanigera/0205E757D51D353A128A93E1779ABF3Dinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199412000068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:51Zoai:ri.conicet.gov.ar:11336/62618instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:51.767CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
title Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
spellingShingle Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
Gramajo Bühler, María Cecilia
Chinchilla Lanigera
Cryopreservation
Dna Fragmentation
Epididymal Sperm
Sperm Quality
title_short Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
title_full Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
title_fullStr Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
title_full_unstemmed Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
title_sort Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera
dc.creator.none.fl_str_mv Gramajo Bühler, María Cecilia
Pucci Alcaide, F.
Seisdedos de Sánchez Toranzo, Graciela del Valle
author Gramajo Bühler, María Cecilia
author_facet Gramajo Bühler, María Cecilia
Pucci Alcaide, F.
Seisdedos de Sánchez Toranzo, Graciela del Valle
author_role author
author2 Pucci Alcaide, F.
Seisdedos de Sánchez Toranzo, Graciela del Valle
author2_role author
author
dc.subject.none.fl_str_mv Chinchilla Lanigera
Cryopreservation
Dna Fragmentation
Epididymal Sperm
Sperm Quality
topic Chinchilla Lanigera
Cryopreservation
Dna Fragmentation
Epididymal Sperm
Sperm Quality
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Summary Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen-thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material. Copyright © Cambridge University Press 2012.
Fil: Gramajo Bühler, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo | Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas. Departamento de Biología del Desarrollo; Argentina
Fil: Pucci Alcaide, F.. Fundación Miguel Lillo; Argentina
Fil: Seisdedos de Sánchez Toranzo, Graciela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Summary Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen-thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material. Copyright © Cambridge University Press 2012.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62618
Gramajo Bühler, María Cecilia; Pucci Alcaide, F.; Seisdedos de Sánchez Toranzo, Graciela del Valle; Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera; Cambridge University Press; Zygote; 21; 4; 11-2013; 351-357
0967-1994
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62618
identifier_str_mv Gramajo Bühler, María Cecilia; Pucci Alcaide, F.; Seisdedos de Sánchez Toranzo, Graciela del Valle; Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera; Cambridge University Press; Zygote; 21; 4; 11-2013; 351-357
0967-1994
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/effect-on-sperm-quality-of-different-cryoprotectants-in-sperm-of-chinchilla-lanigera/0205E757D51D353A128A93E1779ABF3D
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199412000068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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