Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation

Autores
Busso, D.; Cohen, Debora Juana; Da Ros, Vanina Gabriela; Fissore, R.; Cuasnicu, Patricia Sara
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.
Fil: Busso, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fissore, R.. University of Massachussets; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
FERTILIZATION
OOCYTES
EPIDIDYMAL SECRETORY PROTEINS
SPERM CAPACITATION
SPERMATOZOA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activationBusso, D.Cohen, Debora JuanaDa Ros, Vanina GabrielaFissore, R.Cuasnicu, Patricia SaraFERTILIZATIONOOCYTESEPIDIDYMAL SECRETORY PROTEINSSPERM CAPACITATIONSPERMATOZOAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.Fil: Busso, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fissore, R.. University of Massachussets; Estados UnidosFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaC. M. B. Association2003-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36271Busso, D.; Cohen, Debora Juana; Da Ros, Vanina Gabriela; Fissore, R.; Cuasnicu, Patricia Sara; Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation; C. M. B. Association; Cellular and Molecular Biology; 49; 3; 5-2003; 407-4120145-5680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/pmid/12887093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:41Zoai:ri.conicet.gov.ar:11336/36271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:41.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
title Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
spellingShingle Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
Busso, D.
FERTILIZATION
OOCYTES
EPIDIDYMAL SECRETORY PROTEINS
SPERM CAPACITATION
SPERMATOZOA
title_short Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
title_full Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
title_fullStr Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
title_full_unstemmed Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
title_sort Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation
dc.creator.none.fl_str_mv Busso, D.
Cohen, Debora Juana
Da Ros, Vanina Gabriela
Fissore, R.
Cuasnicu, Patricia Sara
author Busso, D.
author_facet Busso, D.
Cohen, Debora Juana
Da Ros, Vanina Gabriela
Fissore, R.
Cuasnicu, Patricia Sara
author_role author
author2 Cohen, Debora Juana
Da Ros, Vanina Gabriela
Fissore, R.
Cuasnicu, Patricia Sara
author2_role author
author
author
author
dc.subject.none.fl_str_mv FERTILIZATION
OOCYTES
EPIDIDYMAL SECRETORY PROTEINS
SPERM CAPACITATION
SPERMATOZOA
topic FERTILIZATION
OOCYTES
EPIDIDYMAL SECRETORY PROTEINS
SPERM CAPACITATION
SPERMATOZOA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.
Fil: Busso, D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fissore, R.. University of Massachussets; Estados Unidos
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.
publishDate 2003
dc.date.none.fl_str_mv 2003-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36271
Busso, D.; Cohen, Debora Juana; Da Ros, Vanina Gabriela; Fissore, R.; Cuasnicu, Patricia Sara; Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation; C. M. B. Association; Cellular and Molecular Biology; 49; 3; 5-2003; 407-412
0145-5680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36271
identifier_str_mv Busso, D.; Cohen, Debora Juana; Da Ros, Vanina Gabriela; Fissore, R.; Cuasnicu, Patricia Sara; Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation; C. M. B. Association; Cellular and Molecular Biology; 49; 3; 5-2003; 407-412
0145-5680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/pmid/12887093
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv C. M. B. Association
publisher.none.fl_str_mv C. M. B. Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269047403577344
score 13.13397

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