Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
- Autores
- Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Rotllant, David. Universitat Autònoma de Barcelona; España
Fil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; España
Fil: Armario, Antonio. Universitat Autònoma de Barcelona; España - Materia
-
ADAPTATION
CHRONIC STRESS
PARAVENTRICULAR HYPOTHALAMIC NUCLEUS
RAT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/242450
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CONICET Digital (CONICET) |
spelling |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptationOns, SheilaRotllant, DavidMarín Blasco, Ignacio J.Armario, AntonioADAPTATIONCHRONIC STRESSPARAVENTRICULAR HYPOTHALAMIC NUCLEUSRAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Rotllant, David. Universitat Autònoma de Barcelona; EspañaFil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; EspañaFil: Armario, Antonio. Universitat Autònoma de Barcelona; EspañaWiley Blackwell Publishing, Inc2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242450Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-20520953-816XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2010.07242.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2010.07242.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:34Zoai:ri.conicet.gov.ar:11336/242450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:35.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
title |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
spellingShingle |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation Ons, Sheila ADAPTATION CHRONIC STRESS PARAVENTRICULAR HYPOTHALAMIC NUCLEUS RAT |
title_short |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
title_full |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
title_fullStr |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
title_full_unstemmed |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
title_sort |
Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation |
dc.creator.none.fl_str_mv |
Ons, Sheila Rotllant, David Marín Blasco, Ignacio J. Armario, Antonio |
author |
Ons, Sheila |
author_facet |
Ons, Sheila Rotllant, David Marín Blasco, Ignacio J. Armario, Antonio |
author_role |
author |
author2 |
Rotllant, David Marín Blasco, Ignacio J. Armario, Antonio |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
ADAPTATION CHRONIC STRESS PARAVENTRICULAR HYPOTHALAMIC NUCLEUS RAT |
topic |
ADAPTATION CHRONIC STRESS PARAVENTRICULAR HYPOTHALAMIC NUCLEUS RAT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation. Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Rotllant, David. Universitat Autònoma de Barcelona; España Fil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; España Fil: Armario, Antonio. Universitat Autònoma de Barcelona; España |
description |
Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/242450 Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-2052 0953-816X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/242450 |
identifier_str_mv |
Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-2052 0953-816X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2010.07242.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2010.07242.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613558858416128 |
score |
13.070432 |