Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation

Autores
Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Rotllant, David. Universitat Autònoma de Barcelona; España
Fil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; España
Fil: Armario, Antonio. Universitat Autònoma de Barcelona; España
Materia
ADAPTATION
CHRONIC STRESS
PARAVENTRICULAR HYPOTHALAMIC NUCLEUS
RAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242450

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spelling Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptationOns, SheilaRotllant, DavidMarín Blasco, Ignacio J.Armario, AntonioADAPTATIONCHRONIC STRESSPARAVENTRICULAR HYPOTHALAMIC NUCLEUSRAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Rotllant, David. Universitat Autònoma de Barcelona; EspañaFil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; EspañaFil: Armario, Antonio. Universitat Autònoma de Barcelona; EspañaWiley Blackwell Publishing, Inc2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242450Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-20520953-816XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2010.07242.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2010.07242.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:34Zoai:ri.conicet.gov.ar:11336/242450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:35.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
title Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
spellingShingle Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
Ons, Sheila
ADAPTATION
CHRONIC STRESS
PARAVENTRICULAR HYPOTHALAMIC NUCLEUS
RAT
title_short Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
title_full Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
title_fullStr Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
title_full_unstemmed Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
title_sort Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation
dc.creator.none.fl_str_mv Ons, Sheila
Rotllant, David
Marín Blasco, Ignacio J.
Armario, Antonio
author Ons, Sheila
author_facet Ons, Sheila
Rotllant, David
Marín Blasco, Ignacio J.
Armario, Antonio
author_role author
author2 Rotllant, David
Marín Blasco, Ignacio J.
Armario, Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv ADAPTATION
CHRONIC STRESS
PARAVENTRICULAR HYPOTHALAMIC NUCLEUS
RAT
topic ADAPTATION
CHRONIC STRESS
PARAVENTRICULAR HYPOTHALAMIC NUCLEUS
RAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.
Fil: Ons, Sheila. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Rotllant, David. Universitat Autònoma de Barcelona; España
Fil: Marín Blasco, Ignacio J.. Universitat Autònoma de Barcelona; España
Fil: Armario, Antonio. Universitat Autònoma de Barcelona; España
description Stress exposure resulted in brain induction of immediate-early genes (IEGs), considered as markers of neuronal activation. Upon repeated exposure to the same stressor, reduction of IEG response (adaptation) has been often observed, but there are important discrepancies in literature that may be in part related to the particular IEG and methodology used. We studied the differential pattern of adaptation of the IEGs c-fos and arc (activity-regulated cytoskeleton-associated protein) after repeated exposure to a severe stressor: immobilization on wooden boards (IMO). Rats repeatedly exposed to IMO showed reduced c-fos mRNA levels in response to acute IMO in most brain areas studied: the medial prefrontal cortex (mPFC), lateral septum (LS), medial amygdala (MeA), paraventricular nucleus of the hypothalamus (PVN) and locus coeruleus. In contrast, the number of neurons showing Fos-like immunoreactivity was only reduced in the MeA and the various subregions of the PVN. IMO-induced increases in arc gene expression were restricted to telencephalic regions and reduced by repeated IMO only in the mPFC. Double-labelling in the LS of IMO-exposed rats revealed that arc was expressed in only one-third of Fos+ neurons, suggesting two populations of Fos+ neurons. These data suggest that c-fos mRNA levels are more affected by repeated IMO than corresponding protein, and that arc gene expression does not reflect adaptation in most brain regions, which may be related to its constitutive expression. Therefore, the choice of a particular IEG and the method of measurement are important for proper interpretation of the impact of chronic repeated stress on brain activation.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242450
Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-2052
0953-816X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242450
identifier_str_mv Ons, Sheila; Rotllant, David; Marín Blasco, Ignacio J.; Armario, Antonio; Immediate‐early gene response to repeated immobilization: Fos protein and arc mRNA levels appear to be less sensitive than c‐fos mRNA to adaptation; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 31; 11; 6-2010; 2043-2052
0953-816X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2010.07242.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2010.07242.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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