Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants

Autores
Mainez, María Esperanza; Müller, Diana María; Murguia, Marcelo Cesar
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of biosurfactants became essential because of its multiple properties and applications. The high toxicity to the environment led to search for new alternatives such as the reduction or replacement by biological surfactants. Because of this, it is in our interest to produce biosurfactants from a non-pathogenic Pseudomonas. We obtained lower values of critical micelle concentration (CMC) from the culture broth than obtained from dodecyl sulfate sodium (SDS) and Pluronic F-68, used as pure surfactans. We found values of critical micellar concentration close to 0.15 mg/L in the purified fraction by adsorption chromatography. We determine by mass spectrometry this strain possibly produces two families of biosurfactants. Majority fraction might be formed by cyclic lipopeptides whose molecular weights could be located in the range of 1100-1200 Da. However, it is necessary perform confirmatory structural studies and to determine the specific structure of these analytes.
Fil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Müller, Diana María. Universidad Nacional del Litoral; Argentina
Fil: Murguia, Marcelo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
BIOSURFACTANTES
GLICOLIPIDOS
BIOPROCESOS
TECNOLOGIA QUIMICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56062
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical SurfactantsMainez, María EsperanzaMüller, Diana MaríaMurguia, Marcelo CesarBIOSURFACTANTESGLICOLIPIDOSBIOPROCESOSTECNOLOGIA QUIMICAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The use of biosurfactants became essential because of its multiple properties and applications. The high toxicity to the environment led to search for new alternatives such as the reduction or replacement by biological surfactants. Because of this, it is in our interest to produce biosurfactants from a non-pathogenic Pseudomonas. We obtained lower values of critical micelle concentration (CMC) from the culture broth than obtained from dodecyl sulfate sodium (SDS) and Pluronic F-68, used as pure surfactans. We found values of critical micellar concentration close to 0.15 mg/L in the purified fraction by adsorption chromatography. We determine by mass spectrometry this strain possibly produces two families of biosurfactants. Majority fraction might be formed by cyclic lipopeptides whose molecular weights could be located in the range of 1100-1200 Da. However, it is necessary perform confirmatory structural studies and to determine the specific structure of these analytes.Fil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Müller, Diana María. Universidad Nacional del Litoral; ArgentinaFil: Murguia, Marcelo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaMukesh Arora2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56062Mainez, María Esperanza; Müller, Diana María; Murguia, Marcelo Cesar; Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants; Mukesh Arora; International Journal of Engineering Research & Science (IJOER); 3; 3; 6-2017; 1-72395-6992CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijoer.com/Paper-March-2017/IJOER-FEB-2017-15.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:46Zoai:ri.conicet.gov.ar:11336/56062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:46.745CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
title Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
spellingShingle Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
Mainez, María Esperanza
BIOSURFACTANTES
GLICOLIPIDOS
BIOPROCESOS
TECNOLOGIA QUIMICA
title_short Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
title_full Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
title_fullStr Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
title_full_unstemmed Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
title_sort Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants
dc.creator.none.fl_str_mv Mainez, María Esperanza
Müller, Diana María
Murguia, Marcelo Cesar
author Mainez, María Esperanza
author_facet Mainez, María Esperanza
Müller, Diana María
Murguia, Marcelo Cesar
author_role author
author2 Müller, Diana María
Murguia, Marcelo Cesar
author2_role author
author
dc.subject.none.fl_str_mv BIOSURFACTANTES
GLICOLIPIDOS
BIOPROCESOS
TECNOLOGIA QUIMICA
topic BIOSURFACTANTES
GLICOLIPIDOS
BIOPROCESOS
TECNOLOGIA QUIMICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of biosurfactants became essential because of its multiple properties and applications. The high toxicity to the environment led to search for new alternatives such as the reduction or replacement by biological surfactants. Because of this, it is in our interest to produce biosurfactants from a non-pathogenic Pseudomonas. We obtained lower values of critical micelle concentration (CMC) from the culture broth than obtained from dodecyl sulfate sodium (SDS) and Pluronic F-68, used as pure surfactans. We found values of critical micellar concentration close to 0.15 mg/L in the purified fraction by adsorption chromatography. We determine by mass spectrometry this strain possibly produces two families of biosurfactants. Majority fraction might be formed by cyclic lipopeptides whose molecular weights could be located in the range of 1100-1200 Da. However, it is necessary perform confirmatory structural studies and to determine the specific structure of these analytes.
Fil: Mainez, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Müller, Diana María. Universidad Nacional del Litoral; Argentina
Fil: Murguia, Marcelo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description The use of biosurfactants became essential because of its multiple properties and applications. The high toxicity to the environment led to search for new alternatives such as the reduction or replacement by biological surfactants. Because of this, it is in our interest to produce biosurfactants from a non-pathogenic Pseudomonas. We obtained lower values of critical micelle concentration (CMC) from the culture broth than obtained from dodecyl sulfate sodium (SDS) and Pluronic F-68, used as pure surfactans. We found values of critical micellar concentration close to 0.15 mg/L in the purified fraction by adsorption chromatography. We determine by mass spectrometry this strain possibly produces two families of biosurfactants. Majority fraction might be formed by cyclic lipopeptides whose molecular weights could be located in the range of 1100-1200 Da. However, it is necessary perform confirmatory structural studies and to determine the specific structure of these analytes.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56062
Mainez, María Esperanza; Müller, Diana María; Murguia, Marcelo Cesar; Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants; Mukesh Arora; International Journal of Engineering Research & Science (IJOER); 3; 3; 6-2017; 1-7
2395-6992
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56062
identifier_str_mv Mainez, María Esperanza; Müller, Diana María; Murguia, Marcelo Cesar; Applied Biotechnology: Isolation and Detection of an Efficient Biosurfactant from Pseudomonas sp. Comparative Studies against Chemical Surfactants; Mukesh Arora; International Journal of Engineering Research & Science (IJOER); 3; 3; 6-2017; 1-7
2395-6992
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ijoer.com/Paper-March-2017/IJOER-FEB-2017-15.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mukesh Arora
publisher.none.fl_str_mv Mukesh Arora
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269116266708992
score 13.13397

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