Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) fro...

Autores
Reina, Silvia Lorena; Sterin, Leonor Josefina; Passafaro, Daniela; Borda, Enri Santiago
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: We demonstrated that serum immunoglobulin G (IgG) from patients with primary Sjögren's syndrome (pSS), interacting with the second extracellular loop of human glandular M3 muscarinic acetylcholine receptors (M3 mAChR), trigger the production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2). Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed in the presence of M3 mAChR synthetic peptide as antigen to detect in serum the autoantibodies. Further, MMP-3 and PGE2 production were determined in the presence of anti-M3 mAChR autoantibodies. Results: An association was observed between serum and anti-M3 mAChR autoantibodies and serum levels of MMP-3 and PGE2 in pSS patients. Thus, we established that serum anti-M3 mAChR autoantibodies, MMP-3 and PGE2 may be considered to be early markers of pSS associated with inflammation. Affinity-purified anti-M3 mAChR peptide IgG from pSS patients, whilst stimulating salivary-gland M3 mAChR, causes an increase in the level of MMP-3 and PGE2 as a result of the activation of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) (but not COX-1). Conclusions: These results provide a novel insight into the role that cholinoceptor antibodies play in the development of glandular inflammation. This is the first report showing that an antibody interacting with glandular mAChR can induce the production of pro-inflammatory mediators (MMP-3/PGE2).
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Autoantibodies
Anti-M3 Peptide Igg
Sjögren'S Syndrome
Pge2
Mmp-3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glandsReina, Silvia LorenaSterin, Leonor JosefinaPassafaro, DanielaBorda, Enri SantiagoAutoantibodiesAnti-M3 Peptide IggSjögren'S SyndromePge2Mmp-3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: We demonstrated that serum immunoglobulin G (IgG) from patients with primary Sjögren's syndrome (pSS), interacting with the second extracellular loop of human glandular M3 muscarinic acetylcholine receptors (M3 mAChR), trigger the production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2). Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed in the presence of M3 mAChR synthetic peptide as antigen to detect in serum the autoantibodies. Further, MMP-3 and PGE2 production were determined in the presence of anti-M3 mAChR autoantibodies. Results: An association was observed between serum and anti-M3 mAChR autoantibodies and serum levels of MMP-3 and PGE2 in pSS patients. Thus, we established that serum anti-M3 mAChR autoantibodies, MMP-3 and PGE2 may be considered to be early markers of pSS associated with inflammation. Affinity-purified anti-M3 mAChR peptide IgG from pSS patients, whilst stimulating salivary-gland M3 mAChR, causes an increase in the level of MMP-3 and PGE2 as a result of the activation of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) (but not COX-1). Conclusions: These results provide a novel insight into the role that cholinoceptor antibodies play in the development of glandular inflammation. This is the first report showing that an antibody interacting with glandular mAChR can induce the production of pro-inflammatory mediators (MMP-3/PGE2).Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPergamon-Elsevier Science Ltd2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16271Reina, Silvia Lorena; Sterin, Leonor Josefina; Passafaro, Daniela; Borda, Enri Santiago; Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 56; 5; 5-2011; 413-4200003-9969enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2010.08.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996910002591info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:59Zoai:ri.conicet.gov.ar:11336/16271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:00.097CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
title Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
spellingShingle Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
Reina, Silvia Lorena
Autoantibodies
Anti-M3 Peptide Igg
Sjögren'S Syndrome
Pge2
Mmp-3
title_short Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
title_full Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
title_fullStr Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
title_full_unstemmed Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
title_sort Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands
dc.creator.none.fl_str_mv Reina, Silvia Lorena
Sterin, Leonor Josefina
Passafaro, Daniela
Borda, Enri Santiago
author Reina, Silvia Lorena
author_facet Reina, Silvia Lorena
Sterin, Leonor Josefina
Passafaro, Daniela
Borda, Enri Santiago
author_role author
author2 Sterin, Leonor Josefina
Passafaro, Daniela
Borda, Enri Santiago
author2_role author
author
author
dc.subject.none.fl_str_mv Autoantibodies
Anti-M3 Peptide Igg
Sjögren'S Syndrome
Pge2
Mmp-3
topic Autoantibodies
Anti-M3 Peptide Igg
Sjögren'S Syndrome
Pge2
Mmp-3
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: We demonstrated that serum immunoglobulin G (IgG) from patients with primary Sjögren's syndrome (pSS), interacting with the second extracellular loop of human glandular M3 muscarinic acetylcholine receptors (M3 mAChR), trigger the production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2). Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed in the presence of M3 mAChR synthetic peptide as antigen to detect in serum the autoantibodies. Further, MMP-3 and PGE2 production were determined in the presence of anti-M3 mAChR autoantibodies. Results: An association was observed between serum and anti-M3 mAChR autoantibodies and serum levels of MMP-3 and PGE2 in pSS patients. Thus, we established that serum anti-M3 mAChR autoantibodies, MMP-3 and PGE2 may be considered to be early markers of pSS associated with inflammation. Affinity-purified anti-M3 mAChR peptide IgG from pSS patients, whilst stimulating salivary-gland M3 mAChR, causes an increase in the level of MMP-3 and PGE2 as a result of the activation of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) (but not COX-1). Conclusions: These results provide a novel insight into the role that cholinoceptor antibodies play in the development of glandular inflammation. This is the first report showing that an antibody interacting with glandular mAChR can induce the production of pro-inflammatory mediators (MMP-3/PGE2).
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: We demonstrated that serum immunoglobulin G (IgG) from patients with primary Sjögren's syndrome (pSS), interacting with the second extracellular loop of human glandular M3 muscarinic acetylcholine receptors (M3 mAChR), trigger the production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2). Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed in the presence of M3 mAChR synthetic peptide as antigen to detect in serum the autoantibodies. Further, MMP-3 and PGE2 production were determined in the presence of anti-M3 mAChR autoantibodies. Results: An association was observed between serum and anti-M3 mAChR autoantibodies and serum levels of MMP-3 and PGE2 in pSS patients. Thus, we established that serum anti-M3 mAChR autoantibodies, MMP-3 and PGE2 may be considered to be early markers of pSS associated with inflammation. Affinity-purified anti-M3 mAChR peptide IgG from pSS patients, whilst stimulating salivary-gland M3 mAChR, causes an increase in the level of MMP-3 and PGE2 as a result of the activation of phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) (but not COX-1). Conclusions: These results provide a novel insight into the role that cholinoceptor antibodies play in the development of glandular inflammation. This is the first report showing that an antibody interacting with glandular mAChR can induce the production of pro-inflammatory mediators (MMP-3/PGE2).
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16271
Reina, Silvia Lorena; Sterin, Leonor Josefina; Passafaro, Daniela; Borda, Enri Santiago; Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 56; 5; 5-2011; 413-420
0003-9969
url http://hdl.handle.net/11336/16271
identifier_str_mv Reina, Silvia Lorena; Sterin, Leonor Josefina; Passafaro, Daniela; Borda, Enri Santiago; Anti-M3 muscarinic cholinergic autoantibodies from patients with primary Sjögren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E2 (PGE2) from the submandibular glands; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 56; 5; 5-2011; 413-420
0003-9969
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2010.08.017
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996910002591
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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