Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients

Autores
Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Materia
IgA
Autoantibodies in sjögren syndrome
Saliva
Nitrites/nitrates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179067

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network_name_str CONICET Digital (CONICET)
spelling Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patientsBorda, Enri SantiagoReina, Silvia LorenaGanzinelli, Sabrina BelenIgAAutoantibodies in sjögren syndromeSalivaNitrites/nitrateshttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaSci Forschen2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179067Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-1292471-657XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biocoreopen.org/ijdoh/Role-of-Anti-Muscarinic-Acetylcholine-Ig-and-anti-autoantibodies-in-whole-saliva-from-Primary-Sjogrens-syndrome-patients.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:10Zoai:ri.conicet.gov.ar:11336/179067instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:10.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
title Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
spellingShingle Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
Borda, Enri Santiago
IgA
Autoantibodies in sjögren syndrome
Saliva
Nitrites/nitrates
title_short Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
title_full Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
title_fullStr Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
title_full_unstemmed Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
title_sort Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
dc.creator.none.fl_str_mv Borda, Enri Santiago
Reina, Silvia Lorena
Ganzinelli, Sabrina Belen
author Borda, Enri Santiago
author_facet Borda, Enri Santiago
Reina, Silvia Lorena
Ganzinelli, Sabrina Belen
author_role author
author2 Reina, Silvia Lorena
Ganzinelli, Sabrina Belen
author2_role author
author
dc.subject.none.fl_str_mv IgA
Autoantibodies in sjögren syndrome
Saliva
Nitrites/nitrates
topic IgA
Autoantibodies in sjögren syndrome
Saliva
Nitrites/nitrates
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
description Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179067
Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-129
2471-657X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179067
identifier_str_mv Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-129
2471-657X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biocoreopen.org/ijdoh/Role-of-Anti-Muscarinic-Acetylcholine-Ig-and-anti-autoantibodies-in-whole-saliva-from-Primary-Sjogrens-syndrome-patients.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sci Forschen
publisher.none.fl_str_mv Sci Forschen
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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