Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
- Autores
- Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina - Materia
-
IgA
Autoantibodies in sjögren syndrome
Saliva
Nitrites/nitrates - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/179067
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CONICET Digital (CONICET) |
spelling |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patientsBorda, Enri SantiagoReina, Silvia LorenaGanzinelli, Sabrina BelenIgAAutoantibodies in sjögren syndromeSalivaNitrites/nitrateshttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaSci Forschen2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179067Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-1292471-657XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biocoreopen.org/ijdoh/Role-of-Anti-Muscarinic-Acetylcholine-Ig-and-anti-autoantibodies-in-whole-saliva-from-Primary-Sjogrens-syndrome-patients.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:10Zoai:ri.conicet.gov.ar:11336/179067instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:10.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
title |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
spellingShingle |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients Borda, Enri Santiago IgA Autoantibodies in sjögren syndrome Saliva Nitrites/nitrates |
title_short |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
title_full |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
title_fullStr |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
title_full_unstemmed |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
title_sort |
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients |
dc.creator.none.fl_str_mv |
Borda, Enri Santiago Reina, Silvia Lorena Ganzinelli, Sabrina Belen |
author |
Borda, Enri Santiago |
author_facet |
Borda, Enri Santiago Reina, Silvia Lorena Ganzinelli, Sabrina Belen |
author_role |
author |
author2 |
Reina, Silvia Lorena Ganzinelli, Sabrina Belen |
author2_role |
author author |
dc.subject.none.fl_str_mv |
IgA Autoantibodies in sjögren syndrome Saliva Nitrites/nitrates |
topic |
IgA Autoantibodies in sjögren syndrome Saliva Nitrites/nitrates |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.5 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects. Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina |
description |
Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/179067 Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-129 2471-657X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/179067 |
identifier_str_mv |
Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-129 2471-657X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://biocoreopen.org/ijdoh/Role-of-Anti-Muscarinic-Acetylcholine-Ig-and-anti-autoantibodies-in-whole-saliva-from-Primary-Sjogrens-syndrome-patients.php |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sci Forschen |
publisher.none.fl_str_mv |
Sci Forschen |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269389085212672 |
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13.13397 |