Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells

Autores
Reina, Silvia Lorena; Sterin Borda, Leonor; Borda, Enri Santiago
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Primary Sjögren´s syndrome (pSS) is an autoimmune disease that targets salivary and lachrymal glands, characterized by anti-cholinergic autoantibodies directed against the M3 muscarinic acetylcholine receptor (mAChR). The aim of this work was to evaluate if cholinergic autoantibodies contained in IgG purified from Sjögren sera could trigger apoptosis of A253 cell line. We also determined if caspase-3 and matrix metalloproteinase 3 (MMP-3) are involved in the induction of A253 cell death. Our results demonstrated that anti-cholinergic autoantibodies stimulate apoptosis and inositol phosphate (InsP) accumulation accompanied by caspase-3 activation and MMP-3 production. All of these effects were blunted by atropine and J104794, indicating that M3 mAChRs are impacted by the anti-cholinergic autoantibodies. The intracellular pathway leading to autoantibody-induced biological effects involves phospholipase C (PLC), calcium/calmodulin (CaM) and extracellular calcium as demonstrated by treatment with U-73122, W-7, verapamil, BAPTA and BAPTA-AM, all of which blocked the effects of the anti-cholinergic autoantibodies. In conclusion, anti-cholinergic autoantibodies in IgG purified from pSS patient´s sera mediates apoptosis of the A253 cell line in an InsP, caspase-3 and MMP-3 dependent manner.
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Materia
APOPTOSIS
AUTOANTIBODIES
CASPASE-3
CHOLINERGIC SYSTEM
IGG
METALLOPROTEINASE-3
PRIMARY SJÖGREN'S SYNDROME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195175
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195175
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cellsReina, Silvia LorenaSterin Borda, LeonorBorda, Enri SantiagoAPOPTOSISAUTOANTIBODIESCASPASE-3CHOLINERGIC SYSTEMIGGMETALLOPROTEINASE-3PRIMARY SJÖGREN'S SYNDROMEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Primary Sjögren´s syndrome (pSS) is an autoimmune disease that targets salivary and lachrymal glands, characterized by anti-cholinergic autoantibodies directed against the M3 muscarinic acetylcholine receptor (mAChR). The aim of this work was to evaluate if cholinergic autoantibodies contained in IgG purified from Sjögren sera could trigger apoptosis of A253 cell line. We also determined if caspase-3 and matrix metalloproteinase 3 (MMP-3) are involved in the induction of A253 cell death. Our results demonstrated that anti-cholinergic autoantibodies stimulate apoptosis and inositol phosphate (InsP) accumulation accompanied by caspase-3 activation and MMP-3 production. All of these effects were blunted by atropine and J104794, indicating that M3 mAChRs are impacted by the anti-cholinergic autoantibodies. The intracellular pathway leading to autoantibody-induced biological effects involves phospholipase C (PLC), calcium/calmodulin (CaM) and extracellular calcium as demonstrated by treatment with U-73122, W-7, verapamil, BAPTA and BAPTA-AM, all of which blocked the effects of the anti-cholinergic autoantibodies. In conclusion, anti-cholinergic autoantibodies in IgG purified from pSS patient´s sera mediates apoptosis of the A253 cell line in an InsP, caspase-3 and MMP-3 dependent manner.Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaAcademic Press Inc Elsevier Science2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195175Reina, Silvia Lorena; Sterin Borda, Leonor; Borda, Enri Santiago; Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells; Academic Press Inc Elsevier Science; Cellular Immunology; 275; 1-2; 4-2012; 33-410008-8749CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0008874912000561info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellimm.2012.03.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:28Zoai:ri.conicet.gov.ar:11336/195175instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:29.108CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
title Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
spellingShingle Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
Reina, Silvia Lorena
APOPTOSIS
AUTOANTIBODIES
CASPASE-3
CHOLINERGIC SYSTEM
IGG
METALLOPROTEINASE-3
PRIMARY SJÖGREN'S SYNDROME
title_short Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
title_full Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
title_fullStr Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
title_full_unstemmed Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
title_sort Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells
dc.creator.none.fl_str_mv Reina, Silvia Lorena
Sterin Borda, Leonor
Borda, Enri Santiago
author Reina, Silvia Lorena
author_facet Reina, Silvia Lorena
Sterin Borda, Leonor
Borda, Enri Santiago
author_role author
author2 Sterin Borda, Leonor
Borda, Enri Santiago
author2_role author
author
dc.subject.none.fl_str_mv APOPTOSIS
AUTOANTIBODIES
CASPASE-3
CHOLINERGIC SYSTEM
IGG
METALLOPROTEINASE-3
PRIMARY SJÖGREN'S SYNDROME
topic APOPTOSIS
AUTOANTIBODIES
CASPASE-3
CHOLINERGIC SYSTEM
IGG
METALLOPROTEINASE-3
PRIMARY SJÖGREN'S SYNDROME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Primary Sjögren´s syndrome (pSS) is an autoimmune disease that targets salivary and lachrymal glands, characterized by anti-cholinergic autoantibodies directed against the M3 muscarinic acetylcholine receptor (mAChR). The aim of this work was to evaluate if cholinergic autoantibodies contained in IgG purified from Sjögren sera could trigger apoptosis of A253 cell line. We also determined if caspase-3 and matrix metalloproteinase 3 (MMP-3) are involved in the induction of A253 cell death. Our results demonstrated that anti-cholinergic autoantibodies stimulate apoptosis and inositol phosphate (InsP) accumulation accompanied by caspase-3 activation and MMP-3 production. All of these effects were blunted by atropine and J104794, indicating that M3 mAChRs are impacted by the anti-cholinergic autoantibodies. The intracellular pathway leading to autoantibody-induced biological effects involves phospholipase C (PLC), calcium/calmodulin (CaM) and extracellular calcium as demonstrated by treatment with U-73122, W-7, verapamil, BAPTA and BAPTA-AM, all of which blocked the effects of the anti-cholinergic autoantibodies. In conclusion, anti-cholinergic autoantibodies in IgG purified from pSS patient´s sera mediates apoptosis of the A253 cell line in an InsP, caspase-3 and MMP-3 dependent manner.
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
description Primary Sjögren´s syndrome (pSS) is an autoimmune disease that targets salivary and lachrymal glands, characterized by anti-cholinergic autoantibodies directed against the M3 muscarinic acetylcholine receptor (mAChR). The aim of this work was to evaluate if cholinergic autoantibodies contained in IgG purified from Sjögren sera could trigger apoptosis of A253 cell line. We also determined if caspase-3 and matrix metalloproteinase 3 (MMP-3) are involved in the induction of A253 cell death. Our results demonstrated that anti-cholinergic autoantibodies stimulate apoptosis and inositol phosphate (InsP) accumulation accompanied by caspase-3 activation and MMP-3 production. All of these effects were blunted by atropine and J104794, indicating that M3 mAChRs are impacted by the anti-cholinergic autoantibodies. The intracellular pathway leading to autoantibody-induced biological effects involves phospholipase C (PLC), calcium/calmodulin (CaM) and extracellular calcium as demonstrated by treatment with U-73122, W-7, verapamil, BAPTA and BAPTA-AM, all of which blocked the effects of the anti-cholinergic autoantibodies. In conclusion, anti-cholinergic autoantibodies in IgG purified from pSS patient´s sera mediates apoptosis of the A253 cell line in an InsP, caspase-3 and MMP-3 dependent manner.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195175
Reina, Silvia Lorena; Sterin Borda, Leonor; Borda, Enri Santiago; Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells; Academic Press Inc Elsevier Science; Cellular Immunology; 275; 1-2; 4-2012; 33-41
0008-8749
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195175
identifier_str_mv Reina, Silvia Lorena; Sterin Borda, Leonor; Borda, Enri Santiago; Anti-M 3 peptide IgG from Sjögren's syndrome triggers apoptosis in A253 cells; Academic Press Inc Elsevier Science; Cellular Immunology; 275; 1-2; 4-2012; 33-41
0008-8749
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0008874912000561
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellimm.2012.03.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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