Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms

Autores
Martinez, A.; Cavello, Ivana Alejandra; Garmendia, G.; Rufo, C.; Cavalitto, Sebastian Fernando; Vero, S.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures. Esterase activity was the most common enzymatic activity expressed by the yeast isolates regardless the assay temperature and inulinase was the second most common enzymatic activity. No cellulolytic activity was detected. One yeast identified as Guehomyces pullulans (8E) showed significant activity across six of seven enzymes types tested. Twenty-eight yeast isolates were classified as oleaginous, being the isolate 8E the strain that accumulated the highest levels of saponifiable lipids (42 %).
Fil: Martinez, A.. Universidad de la República. Facultad de Química; Uruguay
Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Garmendia, G.. Universidad de la República. Facultad de Química; Uruguay
Fil: Rufo, C.. Universidad de la República. Facultad de Química; Uruguay
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Vero, S.. Universidad de la República. Facultad de Química; Uruguay
Materia
Bioenergetics
Enzymes
Psychrophiles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49440
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49440
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganismsMartinez, A.Cavello, Ivana AlejandraGarmendia, G.Rufo, C.Cavalitto, Sebastian FernandoVero, S.BioenergeticsEnzymesPsychrophileshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures. Esterase activity was the most common enzymatic activity expressed by the yeast isolates regardless the assay temperature and inulinase was the second most common enzymatic activity. No cellulolytic activity was detected. One yeast identified as Guehomyces pullulans (8E) showed significant activity across six of seven enzymes types tested. Twenty-eight yeast isolates were classified as oleaginous, being the isolate 8E the strain that accumulated the highest levels of saponifiable lipids (42 %).Fil: Martinez, A.. Universidad de la República. Facultad de Química; UruguayFil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Garmendia, G.. Universidad de la República. Facultad de Química; UruguayFil: Rufo, C.. Universidad de la República. Facultad de Química; UruguayFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Vero, S.. Universidad de la República. Facultad de Química; UruguaySpringer Tokyo2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49440Martinez, A.; Cavello, Ivana Alejandra; Garmendia, G.; Rufo, C.; Cavalitto, Sebastian Fernando; et al.; Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms; Springer Tokyo; Extremophiles; 20; 5; 9-2016; 759-7691431-0651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00792-016-0865-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00792-016-0865-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:10Zoai:ri.conicet.gov.ar:11336/49440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:10.927CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
title Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
spellingShingle Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
Martinez, A.
Bioenergetics
Enzymes
Psychrophiles
title_short Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
title_full Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
title_fullStr Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
title_full_unstemmed Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
title_sort Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
dc.creator.none.fl_str_mv Martinez, A.
Cavello, Ivana Alejandra
Garmendia, G.
Rufo, C.
Cavalitto, Sebastian Fernando
Vero, S.
author Martinez, A.
author_facet Martinez, A.
Cavello, Ivana Alejandra
Garmendia, G.
Rufo, C.
Cavalitto, Sebastian Fernando
Vero, S.
author_role author
author2 Cavello, Ivana Alejandra
Garmendia, G.
Rufo, C.
Cavalitto, Sebastian Fernando
Vero, S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioenergetics
Enzymes
Psychrophiles
topic Bioenergetics
Enzymes
Psychrophiles
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures. Esterase activity was the most common enzymatic activity expressed by the yeast isolates regardless the assay temperature and inulinase was the second most common enzymatic activity. No cellulolytic activity was detected. One yeast identified as Guehomyces pullulans (8E) showed significant activity across six of seven enzymes types tested. Twenty-eight yeast isolates were classified as oleaginous, being the isolate 8E the strain that accumulated the highest levels of saponifiable lipids (42 %).
Fil: Martinez, A.. Universidad de la República. Facultad de Química; Uruguay
Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Garmendia, G.. Universidad de la República. Facultad de Química; Uruguay
Fil: Rufo, C.. Universidad de la República. Facultad de Química; Uruguay
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Vero, S.. Universidad de la República. Facultad de Química; Uruguay
description Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures. Esterase activity was the most common enzymatic activity expressed by the yeast isolates regardless the assay temperature and inulinase was the second most common enzymatic activity. No cellulolytic activity was detected. One yeast identified as Guehomyces pullulans (8E) showed significant activity across six of seven enzymes types tested. Twenty-eight yeast isolates were classified as oleaginous, being the isolate 8E the strain that accumulated the highest levels of saponifiable lipids (42 %).
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49440
Martinez, A.; Cavello, Ivana Alejandra; Garmendia, G.; Rufo, C.; Cavalitto, Sebastian Fernando; et al.; Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms; Springer Tokyo; Extremophiles; 20; 5; 9-2016; 759-769
1431-0651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49440
identifier_str_mv Martinez, A.; Cavello, Ivana Alejandra; Garmendia, G.; Rufo, C.; Cavalitto, Sebastian Fernando; et al.; Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms; Springer Tokyo; Extremophiles; 20; 5; 9-2016; 759-769
1431-0651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00792-016-0865-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00792-016-0865-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Tokyo
publisher.none.fl_str_mv Springer Tokyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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