Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-prom...
- Autores
- Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto
- Año de publicación
- 2001
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina - Materia
-
Purines, Alcohol And Prostate Cancer
Purines, Xanthine Oxidase And Prostate Cancer
Prostatic Ethanol Metabolism
Ethanol, Acetaldehyde And Prostate
Ethanol, Free Radicals And Prostate Cancer
Alcohol, Alcoholism And the Prostate
Alcohol Biotransformation And Prostate Cancer
Ethanol Promotion of Prostate Cancer - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/71924
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oai:ri.conicet.gov.ar:11336/71924 |
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repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effectsCastro, Gerardo DanielDelgado, Aurora MariaCostantini, Martin HernanCastro, Jose AlbertoPurines, Alcohol And Prostate CancerPurines, Xanthine Oxidase And Prostate CancerProstatic Ethanol MetabolismEthanol, Acetaldehyde And ProstateEthanol, Free Radicals And Prostate CancerAlcohol, Alcoholism And the ProstateAlcohol Biotransformation And Prostate CancerEthanol Promotion of Prostate Cancerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaWiley-liss, Inc2001-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71924Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-1190270-3211CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/1520-6866(2001)21:2<109::AID-TCM1>3.0.CO;2-4info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/1520-6866%282001%2921%3A2%3C109%3A%3AAID-TCM1%3E3.0.CO%3B2-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:07Zoai:ri.conicet.gov.ar:11336/71924instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:07.575CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
title |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
spellingShingle |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects Castro, Gerardo Daniel Purines, Alcohol And Prostate Cancer Purines, Xanthine Oxidase And Prostate Cancer Prostatic Ethanol Metabolism Ethanol, Acetaldehyde And Prostate Ethanol, Free Radicals And Prostate Cancer Alcohol, Alcoholism And the Prostate Alcohol Biotransformation And Prostate Cancer Ethanol Promotion of Prostate Cancer |
title_short |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
title_full |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
title_fullStr |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
title_full_unstemmed |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
title_sort |
Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects |
dc.creator.none.fl_str_mv |
Castro, Gerardo Daniel Delgado, Aurora Maria Costantini, Martin Hernan Castro, Jose Alberto |
author |
Castro, Gerardo Daniel |
author_facet |
Castro, Gerardo Daniel Delgado, Aurora Maria Costantini, Martin Hernan Castro, Jose Alberto |
author_role |
author |
author2 |
Delgado, Aurora Maria Costantini, Martin Hernan Castro, Jose Alberto |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Purines, Alcohol And Prostate Cancer Purines, Xanthine Oxidase And Prostate Cancer Prostatic Ethanol Metabolism Ethanol, Acetaldehyde And Prostate Ethanol, Free Radicals And Prostate Cancer Alcohol, Alcoholism And the Prostate Alcohol Biotransformation And Prostate Cancer Ethanol Promotion of Prostate Cancer |
topic |
Purines, Alcohol And Prostate Cancer Purines, Xanthine Oxidase And Prostate Cancer Prostatic Ethanol Metabolism Ethanol, Acetaldehyde And Prostate Ethanol, Free Radicals And Prostate Cancer Alcohol, Alcoholism And the Prostate Alcohol Biotransformation And Prostate Cancer Ethanol Promotion of Prostate Cancer |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient. Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina |
description |
The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/71924 Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-119 0270-3211 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/71924 |
identifier_str_mv |
Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-119 0270-3211 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/1520-6866(2001)21:2<109::AID-TCM1>3.0.CO;2-4 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/1520-6866%282001%2921%3A2%3C109%3A%3AAID-TCM1%3E3.0.CO%3B2-4 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-liss, Inc |
publisher.none.fl_str_mv |
Wiley-liss, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613821714399232 |
score |
13.070432 |