Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-prom...

Autores
Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Materia
Purines, Alcohol And Prostate Cancer
Purines, Xanthine Oxidase And Prostate Cancer
Prostatic Ethanol Metabolism
Ethanol, Acetaldehyde And Prostate
Ethanol, Free Radicals And Prostate Cancer
Alcohol, Alcoholism And the Prostate
Alcohol Biotransformation And Prostate Cancer
Ethanol Promotion of Prostate Cancer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71924
Acceder
id CONICETDig_708a8280349953f1803eafbd97016fd0
oai_identifier_str oai:ri.conicet.gov.ar:11336/71924
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effectsCastro, Gerardo DanielDelgado, Aurora MariaCostantini, Martin HernanCastro, Jose AlbertoPurines, Alcohol And Prostate CancerPurines, Xanthine Oxidase And Prostate CancerProstatic Ethanol MetabolismEthanol, Acetaldehyde And ProstateEthanol, Free Radicals And Prostate CancerAlcohol, Alcoholism And the ProstateAlcohol Biotransformation And Prostate CancerEthanol Promotion of Prostate Cancerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); ArgentinaWiley-liss, Inc2001-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71924Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-1190270-3211CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/1520-6866(2001)21:2<109::AID-TCM1>3.0.CO;2-4info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/1520-6866%282001%2921%3A2%3C109%3A%3AAID-TCM1%3E3.0.CO%3B2-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:11Zoai:ri.conicet.gov.ar:11336/71924instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:11.954CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
title Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
spellingShingle Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
Castro, Gerardo Daniel
Purines, Alcohol And Prostate Cancer
Purines, Xanthine Oxidase And Prostate Cancer
Prostatic Ethanol Metabolism
Ethanol, Acetaldehyde And Prostate
Ethanol, Free Radicals And Prostate Cancer
Alcohol, Alcoholism And the Prostate
Alcohol Biotransformation And Prostate Cancer
Ethanol Promotion of Prostate Cancer
title_short Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
title_full Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
title_fullStr Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
title_full_unstemmed Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
title_sort Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects
dc.creator.none.fl_str_mv Castro, Gerardo Daniel
Delgado, Aurora Maria
Costantini, Martin Hernan
Castro, Jose Alberto
author Castro, Gerardo Daniel
author_facet Castro, Gerardo Daniel
Delgado, Aurora Maria
Costantini, Martin Hernan
Castro, Jose Alberto
author_role author
author2 Delgado, Aurora Maria
Costantini, Martin Hernan
Castro, Jose Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Purines, Alcohol And Prostate Cancer
Purines, Xanthine Oxidase And Prostate Cancer
Prostatic Ethanol Metabolism
Ethanol, Acetaldehyde And Prostate
Ethanol, Free Radicals And Prostate Cancer
Alcohol, Alcoholism And the Prostate
Alcohol Biotransformation And Prostate Cancer
Ethanol Promotion of Prostate Cancer
topic Purines, Alcohol And Prostate Cancer
Purines, Xanthine Oxidase And Prostate Cancer
Prostatic Ethanol Metabolism
Ethanol, Acetaldehyde And Prostate
Ethanol, Free Radicals And Prostate Cancer
Alcohol, Alcoholism And the Prostate
Alcohol Biotransformation And Prostate Cancer
Ethanol Promotion of Prostate Cancer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Costantini, Martin Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. GP. CITEFA - Centro de Investigaciones Toxicológicas (I); Argentina
description The ability of the ventral prostate cytosolic fractions to biotransform ethanol to acetaldehyde and 1‐hydroxyethyl (1HEt) radicals was tested. Acetaldehyde formation was determined by GC‐FID analysis in the head space of incubation mixtures. 1HEt was determined by spin trapping with PBN followed by extraction, silylation of the adduct and GC‐MS of the product. Prostate cytosol was able to biotransform ethanol to acetaldehyde in the presence of NADH, hypoxanthine, xanthine, caffeine, theobromine, theophylline, and 1,7‐dimethylxanthine but not in the presence of N‐methylnicotinamide. All these biotransformations were inhibited by allopurinol and were sensitive to heating for 5 min at 100°C. The biotransformation of ethanol to acetaldehyde in the presence of purines as cosubstrates was accompanied by the formation of hydroxyl and 1HEt radicals as detected by GC‐MS, and the process was inhibited by allopurinol. Results suggest that prostate cytosolic xanthine oxidase is able to bioactivate ethanol to acetaldehyde and free radicals. The potential of these processes to be involved in tumor‐promoting effects of heavy alcohol drinking in conjunction with high meat and/or purines consumption is analyzed. Multifactorial epidemiological studies considering that possibility might be convenient.
publishDate 2001
dc.date.none.fl_str_mv 2001-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71924
Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-119
0270-3211
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71924
identifier_str_mv Castro, Gerardo Daniel; Delgado, Aurora Maria; Costantini, Martin Hernan; Castro, Jose Alberto; Rat ventral prostate xanthine oxidase bioactivation of ethanol to acetaldehyde and 1-hydroxyethyl free radicals: Analysis of its potential role in heavy alcohol drinking tumor-promoting effects; Wiley-liss, Inc; Teratogenesis Carcinogenesis And Mutagenesis; 21; 2; 2-2001; 109-119
0270-3211
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/1520-6866(2001)21:2<109::AID-TCM1>3.0.CO;2-4
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/1520-6866%282001%2921%3A2%3C109%3A%3AAID-TCM1%3E3.0.CO%3B2-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Inc
publisher.none.fl_str_mv Wiley-liss, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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