Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications

Autores
Placido, Alexandra; Oliveira Farias, Emanuel A.; Marani, Mariela Mirta; Vasconcelos, Adreanne G.; Mafud, Ana C.; Mascarenhas, Yvonne P.; Eiras, Carla; Leite, José R. S. A.; Delerue Matos, María C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Peptides are potential candidates to meet the needs of the modern world in relation to diagnosis, disease monitoring, quality control in industry, and more recently, detection of genetically modified organisms (GMOs) and food security through the development of biosensors.1 Cry1Ab16 is a toxin of crystalline insecticidal proteins family that has been widely used in GMOs to gain resistance to pests. Recently, many studies have focused on evaluating the potential environmental impact of this toxin, including the impact on aquatic environments. Moreover, this toxin has been detected in maternal and fetal samples during pesticide exposure studies related to genetically modified food. For the first time, in this study, peptides derived from the immunogenic Cry1Ab16 toxin (from Bacillus thuringiensis) were immobilized as layer-by-layer films. 2 Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342- 354C, was selected for electrochemical characterization. In addition, an attempt was made to optimize the electrochemical signal of the peptide through interspersion with different natural or synthetic polymers. Finally, the system was characterized using various morphological and spectroscopic techniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)n film proved to be an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators.
Fil: Placido, Alexandra. Universidad de Porto; Portugal
Fil: Oliveira Farias, Emanuel A.. Universidad de Porto; Portugal
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Vasconcelos, Adreanne G.. Universidade Federal do Piaui; Brasil
Fil: Mafud, Ana C.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; Brasil
Fil: Mascarenhas, Yvonne P.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; Brasil
Fil: Eiras, Carla. Universidade Federal do Piaui; Brasil
Fil: Leite, José R. S. A.. Universidade Federal do Piaui; Brasil
Fil: Delerue Matos, María C.. Universidad de Porto; Portugal
15° Encontro Peptídico Ibérico; 15th Iberian Peptide Meeting
Porto
Portugal
Universidade do Porto. Faculdade de Ciencias
Materia
BIOSENSORS
PEPTIDES
GMO
LAYER-BY-LAYER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/209116
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/209116
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applicationsPlacido, AlexandraOliveira Farias, Emanuel A.Marani, Mariela MirtaVasconcelos, Adreanne G.Mafud, Ana C.Mascarenhas, Yvonne P.Eiras, CarlaLeite, José R. S. A.Delerue Matos, María C.BIOSENSORSPEPTIDESGMOLAYER-BY-LAYERhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Peptides are potential candidates to meet the needs of the modern world in relation to diagnosis, disease monitoring, quality control in industry, and more recently, detection of genetically modified organisms (GMOs) and food security through the development of biosensors.1 Cry1Ab16 is a toxin of crystalline insecticidal proteins family that has been widely used in GMOs to gain resistance to pests. Recently, many studies have focused on evaluating the potential environmental impact of this toxin, including the impact on aquatic environments. Moreover, this toxin has been detected in maternal and fetal samples during pesticide exposure studies related to genetically modified food. For the first time, in this study, peptides derived from the immunogenic Cry1Ab16 toxin (from Bacillus thuringiensis) were immobilized as layer-by-layer films. 2 Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342- 354C, was selected for electrochemical characterization. In addition, an attempt was made to optimize the electrochemical signal of the peptide through interspersion with different natural or synthetic polymers. Finally, the system was characterized using various morphological and spectroscopic techniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)n film proved to be an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators.Fil: Placido, Alexandra. Universidad de Porto; PortugalFil: Oliveira Farias, Emanuel A.. Universidad de Porto; PortugalFil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Vasconcelos, Adreanne G.. Universidade Federal do Piaui; BrasilFil: Mafud, Ana C.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; BrasilFil: Mascarenhas, Yvonne P.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; BrasilFil: Eiras, Carla. Universidade Federal do Piaui; BrasilFil: Leite, José R. S. A.. Universidade Federal do Piaui; BrasilFil: Delerue Matos, María C.. Universidad de Porto; Portugal15° Encontro Peptídico Ibérico; 15th Iberian Peptide MeetingPortoPortugalUniversidade do Porto. Faculdade de CienciasOrganizing Committee of XV EPI2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/209116Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications; 15° Encontro Peptídico Ibérico; 15th Iberian Peptide Meeting; Porto; Portugal; 2016; 56-56978-989-206374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.fc.up.pt/EPI2016/include/book_of_abstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:27Zoai:ri.conicet.gov.ar:11336/209116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:28.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
title Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
spellingShingle Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
Placido, Alexandra
BIOSENSORS
PEPTIDES
GMO
LAYER-BY-LAYER
title_short Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
title_full Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
title_fullStr Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
title_full_unstemmed Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
title_sort Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications
dc.creator.none.fl_str_mv Placido, Alexandra
Oliveira Farias, Emanuel A.
Marani, Mariela Mirta
Vasconcelos, Adreanne G.
Mafud, Ana C.
Mascarenhas, Yvonne P.
Eiras, Carla
Leite, José R. S. A.
Delerue Matos, María C.
author Placido, Alexandra
author_facet Placido, Alexandra
Oliveira Farias, Emanuel A.
Marani, Mariela Mirta
Vasconcelos, Adreanne G.
Mafud, Ana C.
Mascarenhas, Yvonne P.
Eiras, Carla
Leite, José R. S. A.
Delerue Matos, María C.
author_role author
author2 Oliveira Farias, Emanuel A.
Marani, Mariela Mirta
Vasconcelos, Adreanne G.
Mafud, Ana C.
Mascarenhas, Yvonne P.
Eiras, Carla
Leite, José R. S. A.
Delerue Matos, María C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOSENSORS
PEPTIDES
GMO
LAYER-BY-LAYER
topic BIOSENSORS
PEPTIDES
GMO
LAYER-BY-LAYER
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Peptides are potential candidates to meet the needs of the modern world in relation to diagnosis, disease monitoring, quality control in industry, and more recently, detection of genetically modified organisms (GMOs) and food security through the development of biosensors.1 Cry1Ab16 is a toxin of crystalline insecticidal proteins family that has been widely used in GMOs to gain resistance to pests. Recently, many studies have focused on evaluating the potential environmental impact of this toxin, including the impact on aquatic environments. Moreover, this toxin has been detected in maternal and fetal samples during pesticide exposure studies related to genetically modified food. For the first time, in this study, peptides derived from the immunogenic Cry1Ab16 toxin (from Bacillus thuringiensis) were immobilized as layer-by-layer films. 2 Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342- 354C, was selected for electrochemical characterization. In addition, an attempt was made to optimize the electrochemical signal of the peptide through interspersion with different natural or synthetic polymers. Finally, the system was characterized using various morphological and spectroscopic techniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)n film proved to be an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators.
Fil: Placido, Alexandra. Universidad de Porto; Portugal
Fil: Oliveira Farias, Emanuel A.. Universidad de Porto; Portugal
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Vasconcelos, Adreanne G.. Universidade Federal do Piaui; Brasil
Fil: Mafud, Ana C.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; Brasil
Fil: Mascarenhas, Yvonne P.. Universidade do Sao Paulo. Instituto de Fisica de Sao Carlos; Brasil
Fil: Eiras, Carla. Universidade Federal do Piaui; Brasil
Fil: Leite, José R. S. A.. Universidade Federal do Piaui; Brasil
Fil: Delerue Matos, María C.. Universidad de Porto; Portugal
15° Encontro Peptídico Ibérico; 15th Iberian Peptide Meeting
Porto
Portugal
Universidade do Porto. Faculdade de Ciencias
description Peptides are potential candidates to meet the needs of the modern world in relation to diagnosis, disease monitoring, quality control in industry, and more recently, detection of genetically modified organisms (GMOs) and food security through the development of biosensors.1 Cry1Ab16 is a toxin of crystalline insecticidal proteins family that has been widely used in GMOs to gain resistance to pests. Recently, many studies have focused on evaluating the potential environmental impact of this toxin, including the impact on aquatic environments. Moreover, this toxin has been detected in maternal and fetal samples during pesticide exposure studies related to genetically modified food. For the first time, in this study, peptides derived from the immunogenic Cry1Ab16 toxin (from Bacillus thuringiensis) were immobilized as layer-by-layer films. 2 Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342- 354C, was selected for electrochemical characterization. In addition, an attempt was made to optimize the electrochemical signal of the peptide through interspersion with different natural or synthetic polymers. Finally, the system was characterized using various morphological and spectroscopic techniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)n film proved to be an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/209116
Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications; 15° Encontro Peptídico Ibérico; 15th Iberian Peptide Meeting; Porto; Portugal; 2016; 56-56
978-989-206374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/209116
identifier_str_mv Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications; 15° Encontro Peptídico Ibérico; 15th Iberian Peptide Meeting; Porto; Portugal; 2016; 56-56
978-989-206374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.fc.up.pt/EPI2016/include/book_of_abstracts.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Organizing Committee of XV EPI
publisher.none.fl_str_mv Organizing Committee of XV EPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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