Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions

Autores
López, Silvina; Balatti, Pedro Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
Materia
Ciencias Agrarias
inoculant
molecular markers
rhizobia
inoculante
marcadores moleculares
rizobio
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/101
Acceder
id CICBA_95cd91f483a27b28ba0f425910efbb3b
oai_identifier_str oai:digital.cic.gba.gob.ar:11746/101
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactionsLópez, SilvinaBalatti, Pedro AlbertoCiencias Agrariasinoculantmolecular markersrhizobiainoculantemarcadores molecularesrizobioThe aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/101enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-18T10:05:14Zoai:digital.cic.gba.gob.ar:11746/101Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-18 10:05:14.539CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
spellingShingle Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
López, Silvina
Ciencias Agrarias
inoculant
molecular markers
rhizobia
inoculante
marcadores moleculares
rizobio
title_short Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_fullStr Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full_unstemmed Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_sort Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
dc.creator.none.fl_str_mv López, Silvina
Balatti, Pedro Alberto
author López, Silvina
author_facet López, Silvina
Balatti, Pedro Alberto
author_role author
author2 Balatti, Pedro Alberto
author2_role author
dc.subject.none.fl_str_mv Ciencias Agrarias
inoculant
molecular markers
rhizobia
inoculante
marcadores moleculares
rizobio
topic Ciencias Agrarias
inoculant
molecular markers
rhizobia
inoculante
marcadores moleculares
rizobio
dc.description.none.fl_txt_mv The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
description The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/101
url https://digital.cic.gba.gob.ar/handle/11746/101
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 13.000565

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