Heterogeneous distribution of histone methylation in mature human sperm

Autores
la Spina, Florenza Antonella; Romanato, Marina; Brugo Olmedo, Santiago; de Vicentiis, Sabrina; Julianelli, Vanina Laura; Rivera, Rocío M.; Buffone, Mariano Gabriel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose To analyze the presence of various histone modifications in ejaculated human spermatozoa Methods In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm Conclusions Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility
Fil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vicentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Rivera, Rocío M.. University Of Missouri; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
Epigenetic
Human Sperm
Histone Modifications
Sperm Subpopulations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6284
Acceder
id CONICETDig_6f5bf04cc775ed6fcc5a6c7cd4ce1729
oai_identifier_str oai:ri.conicet.gov.ar:11336/6284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heterogeneous distribution of histone methylation in mature human spermla Spina, Florenza AntonellaRomanato, MarinaBrugo Olmedo, Santiagode Vicentiis, SabrinaJulianelli, Vanina LauraRivera, Rocío M.Buffone, Mariano GabrielEpigeneticHuman SpermHistone ModificationsSperm Subpopulationshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose To analyze the presence of various histone modifications in ejaculated human spermatozoa Methods In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm Conclusions Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertilityFil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Brugo Olmedo, Santiago. Centro Médico Seremas; ArgentinaFil: de Vicentiis, Sabrina. Centro Médico Seremas; ArgentinaFil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Rivera, Rocío M.. University Of Missouri; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaSpringer2014-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6284la Spina, Florenza Antonella; Romanato, Marina; Brugo Olmedo, Santiago; de Vicentiis, Sabrina; Julianelli, Vanina Laura; et al.; Heterogeneous distribution of histone methylation in mature human sperm; Springer; Journal of Assisted Reproduction and Genetics; 31; 1; 31-1-2014; 45-491058-0468enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10815-013-0137-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-013-0137-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:45Zoai:ri.conicet.gov.ar:11336/6284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:45.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterogeneous distribution of histone methylation in mature human sperm
title Heterogeneous distribution of histone methylation in mature human sperm
spellingShingle Heterogeneous distribution of histone methylation in mature human sperm
la Spina, Florenza Antonella
Epigenetic
Human Sperm
Histone Modifications
Sperm Subpopulations
title_short Heterogeneous distribution of histone methylation in mature human sperm
title_full Heterogeneous distribution of histone methylation in mature human sperm
title_fullStr Heterogeneous distribution of histone methylation in mature human sperm
title_full_unstemmed Heterogeneous distribution of histone methylation in mature human sperm
title_sort Heterogeneous distribution of histone methylation in mature human sperm
dc.creator.none.fl_str_mv la Spina, Florenza Antonella
Romanato, Marina
Brugo Olmedo, Santiago
de Vicentiis, Sabrina
Julianelli, Vanina Laura
Rivera, Rocío M.
Buffone, Mariano Gabriel
author la Spina, Florenza Antonella
author_facet la Spina, Florenza Antonella
Romanato, Marina
Brugo Olmedo, Santiago
de Vicentiis, Sabrina
Julianelli, Vanina Laura
Rivera, Rocío M.
Buffone, Mariano Gabriel
author_role author
author2 Romanato, Marina
Brugo Olmedo, Santiago
de Vicentiis, Sabrina
Julianelli, Vanina Laura
Rivera, Rocío M.
Buffone, Mariano Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Epigenetic
Human Sperm
Histone Modifications
Sperm Subpopulations
topic Epigenetic
Human Sperm
Histone Modifications
Sperm Subpopulations
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose To analyze the presence of various histone modifications in ejaculated human spermatozoa Methods In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm Conclusions Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility
Fil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vicentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Rivera, Rocío M.. University Of Missouri; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Purpose To analyze the presence of various histone modifications in ejaculated human spermatozoa Methods In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm Conclusions Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility
publishDate 2014
dc.date.none.fl_str_mv 2014-01-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6284
la Spina, Florenza Antonella; Romanato, Marina; Brugo Olmedo, Santiago; de Vicentiis, Sabrina; Julianelli, Vanina Laura; et al.; Heterogeneous distribution of histone methylation in mature human sperm; Springer; Journal of Assisted Reproduction and Genetics; 31; 1; 31-1-2014; 45-49
1058-0468
url http://hdl.handle.net/11336/6284
identifier_str_mv la Spina, Florenza Antonella; Romanato, Marina; Brugo Olmedo, Santiago; de Vicentiis, Sabrina; Julianelli, Vanina Laura; et al.; Heterogeneous distribution of histone methylation in mature human sperm; Springer; Journal of Assisted Reproduction and Genetics; 31; 1; 31-1-2014; 45-49
1058-0468
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10815-013-0137-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-013-0137-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269482351853568
score 13.13397

Publicaciones similares