Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring
- Autores
- Looby, Nikita T.; Tascon, Marcos; Acquaro, Vinicius R.; Reyes Garcés, Nathaly; Vasiljevic, Tijana; Gomez Rios, German Augusto; Wasowicz, Marcin; Pawliszyn, Janusz
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability. High plasma concentrations have been associated with post-operative seizures. Due to the difficulties with maintaining acceptable concentrations of TXA during surgery, implementation of a point-of-care strategy for testing TXA plasma concentration would allow for close monitoring of its concentration during administration. This would facilitate timely corrections to the dosing schedule, and in effect tailor treatment for individual patient needs. In this work, a method for the rapid monitoring of TXA from plasma samples was subsequently carried out via biocompatible solid-phase microextraction (Bio-SPME) coupled directly to tandem mass spectrometry via a microfluidic open interface (MOI). MOI operates under the concept of a flow-isolated desorption volume and was designed with aims to directly hyphenate Bio-SPME to different detection and ionization systems. In addition, it allows the desorption of Bio-SPME fibers in small volumes while it concurrently continues feeding the ESI with a constant flow to minimize cross-talking and instabilities. The methodology was used to monitor six patients with varying degrees of renal dysfunction, at different time points during cardiac surgery. MOI proves to be a reliable and feasible tool for rapid therapeutic drug monitoring. Affording total times of analysis as low as 30 seconds per sample in its high throughput mode configuration while the single sample turn-around time was 15 minutes, including sample preparation. In addition, cross-validation against a standard thin film solid phase microextraction using liquid chromatography coupled to tandem mass spectrometry (TFME-LC-MS/MS) method was performed. Bland-Altman analysis was used to cross-validate the results obtained by the two methods. Data analysis demonstrated that 92% of the compared data pairs (n = 63) were distributed within the acceptable range. The data was also validated by the Passing Bablok regression, demonstrating good statistical agreement between these two methods. Finally, the currently presented method offers comparable results to the conventional liquid chromatography with acceptable RSDs, while only necessitating a fraction of the time. In this way, TXA concentration in plasma can be monitored in a close to real time throughput during surgery.
Fil: Looby, Nikita T.. University of Waterloo; Canadá
Fil: Tascon, Marcos. University of Waterloo; Canadá. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Acquaro, Vinicius R.. Universidade de Sao Paulo; Brasil. University of Waterloo; Canadá
Fil: Reyes Garcés, Nathaly. Restek Corporation; Estados Unidos. University of Waterloo; Canadá
Fil: Vasiljevic, Tijana. University of Waterloo; Canadá
Fil: Gomez Rios, German Augusto. University of Waterloo; Canadá. Restek Corporation; Estados Unidos
Fil: Wasowicz, Marcin. Toronto General Hospital; Canadá
Fil: Pawliszyn, Janusz. University of Waterloo; Canadá - Materia
-
SPME-MS
MICROFLUIDIC OPEN INTERFACE
BIOANALYSIS
TRANEXAMIC ACID - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/122804
Ver los metadatos del registro completo
id |
CONICETDig_6f5689ed21670493926991d91d442010 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/122804 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoringLooby, Nikita T.Tascon, MarcosAcquaro, Vinicius R.Reyes Garcés, NathalyVasiljevic, TijanaGomez Rios, German AugustoWasowicz, MarcinPawliszyn, JanuszSPME-MSMICROFLUIDIC OPEN INTERFACEBIOANALYSISTRANEXAMIC ACIDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability. High plasma concentrations have been associated with post-operative seizures. Due to the difficulties with maintaining acceptable concentrations of TXA during surgery, implementation of a point-of-care strategy for testing TXA plasma concentration would allow for close monitoring of its concentration during administration. This would facilitate timely corrections to the dosing schedule, and in effect tailor treatment for individual patient needs. In this work, a method for the rapid monitoring of TXA from plasma samples was subsequently carried out via biocompatible solid-phase microextraction (Bio-SPME) coupled directly to tandem mass spectrometry via a microfluidic open interface (MOI). MOI operates under the concept of a flow-isolated desorption volume and was designed with aims to directly hyphenate Bio-SPME to different detection and ionization systems. In addition, it allows the desorption of Bio-SPME fibers in small volumes while it concurrently continues feeding the ESI with a constant flow to minimize cross-talking and instabilities. The methodology was used to monitor six patients with varying degrees of renal dysfunction, at different time points during cardiac surgery. MOI proves to be a reliable and feasible tool for rapid therapeutic drug monitoring. Affording total times of analysis as low as 30 seconds per sample in its high throughput mode configuration while the single sample turn-around time was 15 minutes, including sample preparation. In addition, cross-validation against a standard thin film solid phase microextraction using liquid chromatography coupled to tandem mass spectrometry (TFME-LC-MS/MS) method was performed. Bland-Altman analysis was used to cross-validate the results obtained by the two methods. Data analysis demonstrated that 92% of the compared data pairs (n = 63) were distributed within the acceptable range. The data was also validated by the Passing Bablok regression, demonstrating good statistical agreement between these two methods. Finally, the currently presented method offers comparable results to the conventional liquid chromatography with acceptable RSDs, while only necessitating a fraction of the time. In this way, TXA concentration in plasma can be monitored in a close to real time throughput during surgery.Fil: Looby, Nikita T.. University of Waterloo; CanadáFil: Tascon, Marcos. University of Waterloo; Canadá. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Acquaro, Vinicius R.. Universidade de Sao Paulo; Brasil. University of Waterloo; CanadáFil: Reyes Garcés, Nathaly. Restek Corporation; Estados Unidos. University of Waterloo; CanadáFil: Vasiljevic, Tijana. University of Waterloo; CanadáFil: Gomez Rios, German Augusto. University of Waterloo; Canadá. Restek Corporation; Estados UnidosFil: Wasowicz, Marcin. Toronto General Hospital; CanadáFil: Pawliszyn, Janusz. University of Waterloo; CanadáRoyal Society of Chemistry2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122804Looby, Nikita T.; Tascon, Marcos; Acquaro, Vinicius R.; Reyes Garcés, Nathaly; Vasiljevic, Tijana; et al.; Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring; Royal Society of Chemistry; Analyst; 144; 12; 6-2019; 3721-37280003-2654CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c9an00041kinfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2019/AN/C9AN00041Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:08Zoai:ri.conicet.gov.ar:11336/122804instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:08.578CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
title |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
spellingShingle |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring Looby, Nikita T. SPME-MS MICROFLUIDIC OPEN INTERFACE BIOANALYSIS TRANEXAMIC ACID |
title_short |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
title_full |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
title_fullStr |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
title_full_unstemmed |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
title_sort |
Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring |
dc.creator.none.fl_str_mv |
Looby, Nikita T. Tascon, Marcos Acquaro, Vinicius R. Reyes Garcés, Nathaly Vasiljevic, Tijana Gomez Rios, German Augusto Wasowicz, Marcin Pawliszyn, Janusz |
author |
Looby, Nikita T. |
author_facet |
Looby, Nikita T. Tascon, Marcos Acquaro, Vinicius R. Reyes Garcés, Nathaly Vasiljevic, Tijana Gomez Rios, German Augusto Wasowicz, Marcin Pawliszyn, Janusz |
author_role |
author |
author2 |
Tascon, Marcos Acquaro, Vinicius R. Reyes Garcés, Nathaly Vasiljevic, Tijana Gomez Rios, German Augusto Wasowicz, Marcin Pawliszyn, Janusz |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
SPME-MS MICROFLUIDIC OPEN INTERFACE BIOANALYSIS TRANEXAMIC ACID |
topic |
SPME-MS MICROFLUIDIC OPEN INTERFACE BIOANALYSIS TRANEXAMIC ACID |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability. High plasma concentrations have been associated with post-operative seizures. Due to the difficulties with maintaining acceptable concentrations of TXA during surgery, implementation of a point-of-care strategy for testing TXA plasma concentration would allow for close monitoring of its concentration during administration. This would facilitate timely corrections to the dosing schedule, and in effect tailor treatment for individual patient needs. In this work, a method for the rapid monitoring of TXA from plasma samples was subsequently carried out via biocompatible solid-phase microextraction (Bio-SPME) coupled directly to tandem mass spectrometry via a microfluidic open interface (MOI). MOI operates under the concept of a flow-isolated desorption volume and was designed with aims to directly hyphenate Bio-SPME to different detection and ionization systems. In addition, it allows the desorption of Bio-SPME fibers in small volumes while it concurrently continues feeding the ESI with a constant flow to minimize cross-talking and instabilities. The methodology was used to monitor six patients with varying degrees of renal dysfunction, at different time points during cardiac surgery. MOI proves to be a reliable and feasible tool for rapid therapeutic drug monitoring. Affording total times of analysis as low as 30 seconds per sample in its high throughput mode configuration while the single sample turn-around time was 15 minutes, including sample preparation. In addition, cross-validation against a standard thin film solid phase microextraction using liquid chromatography coupled to tandem mass spectrometry (TFME-LC-MS/MS) method was performed. Bland-Altman analysis was used to cross-validate the results obtained by the two methods. Data analysis demonstrated that 92% of the compared data pairs (n = 63) were distributed within the acceptable range. The data was also validated by the Passing Bablok regression, demonstrating good statistical agreement between these two methods. Finally, the currently presented method offers comparable results to the conventional liquid chromatography with acceptable RSDs, while only necessitating a fraction of the time. In this way, TXA concentration in plasma can be monitored in a close to real time throughput during surgery. Fil: Looby, Nikita T.. University of Waterloo; Canadá Fil: Tascon, Marcos. University of Waterloo; Canadá. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Acquaro, Vinicius R.. Universidade de Sao Paulo; Brasil. University of Waterloo; Canadá Fil: Reyes Garcés, Nathaly. Restek Corporation; Estados Unidos. University of Waterloo; Canadá Fil: Vasiljevic, Tijana. University of Waterloo; Canadá Fil: Gomez Rios, German Augusto. University of Waterloo; Canadá. Restek Corporation; Estados Unidos Fil: Wasowicz, Marcin. Toronto General Hospital; Canadá Fil: Pawliszyn, Janusz. University of Waterloo; Canadá |
description |
Tranexamic acid (TXA) is an antifibrinolytic used during cardiac surgery that presents high inter-patient variability. High plasma concentrations have been associated with post-operative seizures. Due to the difficulties with maintaining acceptable concentrations of TXA during surgery, implementation of a point-of-care strategy for testing TXA plasma concentration would allow for close monitoring of its concentration during administration. This would facilitate timely corrections to the dosing schedule, and in effect tailor treatment for individual patient needs. In this work, a method for the rapid monitoring of TXA from plasma samples was subsequently carried out via biocompatible solid-phase microextraction (Bio-SPME) coupled directly to tandem mass spectrometry via a microfluidic open interface (MOI). MOI operates under the concept of a flow-isolated desorption volume and was designed with aims to directly hyphenate Bio-SPME to different detection and ionization systems. In addition, it allows the desorption of Bio-SPME fibers in small volumes while it concurrently continues feeding the ESI with a constant flow to minimize cross-talking and instabilities. The methodology was used to monitor six patients with varying degrees of renal dysfunction, at different time points during cardiac surgery. MOI proves to be a reliable and feasible tool for rapid therapeutic drug monitoring. Affording total times of analysis as low as 30 seconds per sample in its high throughput mode configuration while the single sample turn-around time was 15 minutes, including sample preparation. In addition, cross-validation against a standard thin film solid phase microextraction using liquid chromatography coupled to tandem mass spectrometry (TFME-LC-MS/MS) method was performed. Bland-Altman analysis was used to cross-validate the results obtained by the two methods. Data analysis demonstrated that 92% of the compared data pairs (n = 63) were distributed within the acceptable range. The data was also validated by the Passing Bablok regression, demonstrating good statistical agreement between these two methods. Finally, the currently presented method offers comparable results to the conventional liquid chromatography with acceptable RSDs, while only necessitating a fraction of the time. In this way, TXA concentration in plasma can be monitored in a close to real time throughput during surgery. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/122804 Looby, Nikita T.; Tascon, Marcos; Acquaro, Vinicius R.; Reyes Garcés, Nathaly; Vasiljevic, Tijana; et al.; Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring; Royal Society of Chemistry; Analyst; 144; 12; 6-2019; 3721-3728 0003-2654 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/122804 |
identifier_str_mv |
Looby, Nikita T.; Tascon, Marcos; Acquaro, Vinicius R.; Reyes Garcés, Nathaly; Vasiljevic, Tijana; et al.; Solid phase microextraction coupled to mass spectrometry: Via a microfluidic open interface for rapid therapeutic drug monitoring; Royal Society of Chemistry; Analyst; 144; 12; 6-2019; 3721-3728 0003-2654 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1039/c9an00041k info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2019/AN/C9AN00041K |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614320952967168 |
score |
13.070432 |