A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections

Autores
Osterman, Andreas; Vizoso Pinto, María Guadalupe; Jung, Jette; Jaeger, Gundula; Eberle, Josef; Nitschko, Hans; Baiker, Armin
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatitis E Virus (HEV) causes epidemic infections in regions of poor hygiene in the developing world. Over the last years, however, increasing numbers of autochthonous infections in industrialized countries have been described, leading to new interest in this pathogen. Currently available serological test formats to detect IgG and IgM antibodies are mainly based on bacterially expressed ORF2 and ORF3 antigens and often give ambiguous results. The objective of this study was the development of a different assay format for HEV diagnosis—a HEV immunofluorescence test (HEV-IFT) based on mammalian cells transiently expressing recombinant HEV ORF2 protein with a simple production and staining protocol and the investigation of its performance and methodical feasibility under diagnostic laboratory conditions. 31 sera of patients at different phases of HEV infection and 40 control sera from a non-endemic region were analyzed for anti-HEV IgG, IgM, and IgA antibodies. The HEV-IFT detected successfully anti-HEV IgG and IgA, but not anti-HEV IgM antibodies. In the study group the HEV-IFT was able to confirm HEV infections and to support diagnosis when ambiguous results were obtained by commercial assays. Signal localization and staining patterns helped to gather additional information about reactive antibodies present in patient sera. In conclusion the developed IFT for the detection of anti-HEV IgG and IgA antibodies can be used for diagnosis and for the serological confirmation of HEV infections.
Fil: Osterman, Andreas. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Fil: Jung, Jette. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Jaeger, Gundula. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Eberle, Josef. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Nitschko, Hans. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Baiker, Armin. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Bavarian Health and Food Safety Authority; Alemania
Materia
Hepatitis e Virus
Immunofluorescense
Serological Test
Orf2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7170
Acceder
id CONICETDig_6f0ec75c1bff46635deb439abd512e88
oai_identifier_str oai:ri.conicet.gov.ar:11336/7170
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infectionsOsterman, AndreasVizoso Pinto, María GuadalupeJung, JetteJaeger, GundulaEberle, JosefNitschko, HansBaiker, ArminHepatitis e VirusImmunofluorescenseSerological TestOrf2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Hepatitis E Virus (HEV) causes epidemic infections in regions of poor hygiene in the developing world. Over the last years, however, increasing numbers of autochthonous infections in industrialized countries have been described, leading to new interest in this pathogen. Currently available serological test formats to detect IgG and IgM antibodies are mainly based on bacterially expressed ORF2 and ORF3 antigens and often give ambiguous results. The objective of this study was the development of a different assay format for HEV diagnosis—a HEV immunofluorescence test (HEV-IFT) based on mammalian cells transiently expressing recombinant HEV ORF2 protein with a simple production and staining protocol and the investigation of its performance and methodical feasibility under diagnostic laboratory conditions. 31 sera of patients at different phases of HEV infection and 40 control sera from a non-endemic region were analyzed for anti-HEV IgG, IgM, and IgA antibodies. The HEV-IFT detected successfully anti-HEV IgG and IgA, but not anti-HEV IgM antibodies. In the study group the HEV-IFT was able to confirm HEV infections and to support diagnosis when ambiguous results were obtained by commercial assays. Signal localization and staining patterns helped to gather additional information about reactive antibodies present in patient sera. In conclusion the developed IFT for the detection of anti-HEV IgG and IgA antibodies can be used for diagnosis and for the serological confirmation of HEV infections.Fil: Osterman, Andreas. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; AlemaniaFil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; ArgentinaFil: Jung, Jette. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; AlemaniaFil: Jaeger, Gundula. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; AlemaniaFil: Eberle, Josef. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; AlemaniaFil: Nitschko, Hans. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; AlemaniaFil: Baiker, Armin. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Bavarian Health and Food Safety Authority; AlemaniaElsevier2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7170Osterman, Andreas; Vizoso Pinto, María Guadalupe; Jung, Jette; Jaeger, Gundula; Eberle, Josef; et al.; A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections; Elsevier; Journal Of Virological Methods; 191; 1; 4-2013; 48-540166-0934enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2013.03.022info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413000980info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:01Zoai:ri.conicet.gov.ar:11336/7170instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:02.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
title A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
spellingShingle A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
Osterman, Andreas
Hepatitis e Virus
Immunofluorescense
Serological Test
Orf2
title_short A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
title_full A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
title_fullStr A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
title_full_unstemmed A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
title_sort A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections
dc.creator.none.fl_str_mv Osterman, Andreas
Vizoso Pinto, María Guadalupe
Jung, Jette
Jaeger, Gundula
Eberle, Josef
Nitschko, Hans
Baiker, Armin
author Osterman, Andreas
author_facet Osterman, Andreas
Vizoso Pinto, María Guadalupe
Jung, Jette
Jaeger, Gundula
Eberle, Josef
Nitschko, Hans
Baiker, Armin
author_role author
author2 Vizoso Pinto, María Guadalupe
Jung, Jette
Jaeger, Gundula
Eberle, Josef
Nitschko, Hans
Baiker, Armin
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hepatitis e Virus
Immunofluorescense
Serological Test
Orf2
topic Hepatitis e Virus
Immunofluorescense
Serological Test
Orf2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hepatitis E Virus (HEV) causes epidemic infections in regions of poor hygiene in the developing world. Over the last years, however, increasing numbers of autochthonous infections in industrialized countries have been described, leading to new interest in this pathogen. Currently available serological test formats to detect IgG and IgM antibodies are mainly based on bacterially expressed ORF2 and ORF3 antigens and often give ambiguous results. The objective of this study was the development of a different assay format for HEV diagnosis—a HEV immunofluorescence test (HEV-IFT) based on mammalian cells transiently expressing recombinant HEV ORF2 protein with a simple production and staining protocol and the investigation of its performance and methodical feasibility under diagnostic laboratory conditions. 31 sera of patients at different phases of HEV infection and 40 control sera from a non-endemic region were analyzed for anti-HEV IgG, IgM, and IgA antibodies. The HEV-IFT detected successfully anti-HEV IgG and IgA, but not anti-HEV IgM antibodies. In the study group the HEV-IFT was able to confirm HEV infections and to support diagnosis when ambiguous results were obtained by commercial assays. Signal localization and staining patterns helped to gather additional information about reactive antibodies present in patient sera. In conclusion the developed IFT for the detection of anti-HEV IgG and IgA antibodies can be used for diagnosis and for the serological confirmation of HEV infections.
Fil: Osterman, Andreas. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
Fil: Jung, Jette. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Jaeger, Gundula. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Eberle, Josef. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Nitschko, Hans. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Baiker, Armin. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania. Bavarian Health and Food Safety Authority; Alemania
description Hepatitis E Virus (HEV) causes epidemic infections in regions of poor hygiene in the developing world. Over the last years, however, increasing numbers of autochthonous infections in industrialized countries have been described, leading to new interest in this pathogen. Currently available serological test formats to detect IgG and IgM antibodies are mainly based on bacterially expressed ORF2 and ORF3 antigens and often give ambiguous results. The objective of this study was the development of a different assay format for HEV diagnosis—a HEV immunofluorescence test (HEV-IFT) based on mammalian cells transiently expressing recombinant HEV ORF2 protein with a simple production and staining protocol and the investigation of its performance and methodical feasibility under diagnostic laboratory conditions. 31 sera of patients at different phases of HEV infection and 40 control sera from a non-endemic region were analyzed for anti-HEV IgG, IgM, and IgA antibodies. The HEV-IFT detected successfully anti-HEV IgG and IgA, but not anti-HEV IgM antibodies. In the study group the HEV-IFT was able to confirm HEV infections and to support diagnosis when ambiguous results were obtained by commercial assays. Signal localization and staining patterns helped to gather additional information about reactive antibodies present in patient sera. In conclusion the developed IFT for the detection of anti-HEV IgG and IgA antibodies can be used for diagnosis and for the serological confirmation of HEV infections.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7170
Osterman, Andreas; Vizoso Pinto, María Guadalupe; Jung, Jette; Jaeger, Gundula; Eberle, Josef; et al.; A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections; Elsevier; Journal Of Virological Methods; 191; 1; 4-2013; 48-54
0166-0934
url http://hdl.handle.net/11336/7170
identifier_str_mv Osterman, Andreas; Vizoso Pinto, María Guadalupe; Jung, Jette; Jaeger, Gundula; Eberle, Josef; et al.; A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections; Elsevier; Journal Of Virological Methods; 191; 1; 4-2013; 48-54
0166-0934
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2013.03.022
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413000980
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980306429149184
score 12.993085

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