Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)

Autores
Mariqueo, Betsabé L.; Diaz Guevara, María Clara; Rosales, Gabriel; Filippa, Veronica Palmira; Perez, Edith; Mohamed, Fabian Heber
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The pars nervosa (PN) of the neurohypophysis is mainly constituted by pituicytes and other neuroglial cells such as microglial cells, astrocytes and oligodendrocytes, endothelial cells of blood vessels and amylinic axons. The PN pituicytes express the S-100 protein that has a regulatory role within the cell and on different target cells. The aim of this work was to study the seasonal expression of S100 protein in pituitary PN of adult male viscachas (Lagostomus maximus maximus). The animals were captured in their habitat during the most representative months of their annual reproductive cycle. This cycle has three periods: reproductive (RepP), gonadal regression (RegP), and gonadal recovery (RecP). Four pituitary glands of each period were removed and processed for optical microscopy. The S-100 protein was used as markers of pituicytes and their expression was detected by immunohistochemistry. The primary antibody used was the rabbit polyclonal anti-S-100 protein. Immunohistochemical staining was performed using a streptavidinbiotin-peroxidase complex method with the 3, 3’- diaminobenzidine tetrahydrochloride (DAB) as chromogen. A morphometric study was performed and the percentage of immunopositive area for S-100 (% IA-S-100) was measured. The results obtained from these periods were statistically analyzed. Most of the pituicytes presented a cytoplamic immunostaining pattern. However, some of them also exhibited nuclear staining. The nuclei were round, oval and irregular in shape, with varying density of chromatin and an evident nucleolus. Numerous immunostained cytoplasmic processes in contact with blood vessels were observed. The % IA-S-100 varied throughout the annual reproductive cycle. The % IA during the RepP (13.09 ± 1.49) and RegP (9.77 ± 1.56) were significantly lower than in the RecP (25.61 ± 2.36; P < 0.001). These results demonstrated that the highest expression of S-100 protein was in the RecP. This period agrees with the increase of seasonal rainfall pattern during spring, so the viscachas have greater water availability in the environment. It is likely that the pituicytes participate in the seasonal regulation of the neurohormonal secretion through the expression of the S-100 protein. This protein is associated with functions such as modulation of enzymatic activity, stimulation of adenylate cyclase, maintenance of cell shape and mobility. However, further research is needed to elucidate the relationship between S-100 expression and the neurohormonal PN secretion of Lagostomus.
Fil: Mariqueo, Betsabé L.. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Diaz Guevara, María Clara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rosales, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina
Fil: Perez, Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias Biológicas
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
LAGOSTOMUS
PITUITARY
S-100 PROTEIN
SEASONAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159993
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/159993
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)Mariqueo, Betsabé L.Diaz Guevara, María ClaraRosales, GabrielFilippa, Veronica PalmiraPerez, EdithMohamed, Fabian HeberLAGOSTOMUSPITUITARYS-100 PROTEINSEASONALhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The pars nervosa (PN) of the neurohypophysis is mainly constituted by pituicytes and other neuroglial cells such as microglial cells, astrocytes and oligodendrocytes, endothelial cells of blood vessels and amylinic axons. The PN pituicytes express the S-100 protein that has a regulatory role within the cell and on different target cells. The aim of this work was to study the seasonal expression of S100 protein in pituitary PN of adult male viscachas (Lagostomus maximus maximus). The animals were captured in their habitat during the most representative months of their annual reproductive cycle. This cycle has three periods: reproductive (RepP), gonadal regression (RegP), and gonadal recovery (RecP). Four pituitary glands of each period were removed and processed for optical microscopy. The S-100 protein was used as markers of pituicytes and their expression was detected by immunohistochemistry. The primary antibody used was the rabbit polyclonal anti-S-100 protein. Immunohistochemical staining was performed using a streptavidinbiotin-peroxidase complex method with the 3, 3’- diaminobenzidine tetrahydrochloride (DAB) as chromogen. A morphometric study was performed and the percentage of immunopositive area for S-100 (% IA-S-100) was measured. The results obtained from these periods were statistically analyzed. Most of the pituicytes presented a cytoplamic immunostaining pattern. However, some of them also exhibited nuclear staining. The nuclei were round, oval and irregular in shape, with varying density of chromatin and an evident nucleolus. Numerous immunostained cytoplasmic processes in contact with blood vessels were observed. The % IA-S-100 varied throughout the annual reproductive cycle. The % IA during the RepP (13.09 ± 1.49) and RegP (9.77 ± 1.56) were significantly lower than in the RecP (25.61 ± 2.36; P < 0.001). These results demonstrated that the highest expression of S-100 protein was in the RecP. This period agrees with the increase of seasonal rainfall pattern during spring, so the viscachas have greater water availability in the environment. It is likely that the pituicytes participate in the seasonal regulation of the neurohormonal secretion through the expression of the S-100 protein. This protein is associated with functions such as modulation of enzymatic activity, stimulation of adenylate cyclase, maintenance of cell shape and mobility. However, further research is needed to elucidate the relationship between S-100 expression and the neurohormonal PN secretion of Lagostomus.Fil: Mariqueo, Betsabé L.. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Diaz Guevara, María Clara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Rosales, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; ArgentinaFil: Perez, Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias BiológicasSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159993Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus); XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias Biológicas; San Luis; Argentina; 2019; 61-61CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/xxxv-reunion-cientifica-anual/#:~:text=Lugar%20y%20Fecha,Descargar%20mapa.Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:49Zoai:ri.conicet.gov.ar:11336/159993instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:49.47CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
title Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
spellingShingle Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
Mariqueo, Betsabé L.
LAGOSTOMUS
PITUITARY
S-100 PROTEIN
SEASONAL
title_short Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
title_full Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
title_fullStr Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
title_full_unstemmed Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
title_sort Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus)
dc.creator.none.fl_str_mv Mariqueo, Betsabé L.
Diaz Guevara, María Clara
Rosales, Gabriel
Filippa, Veronica Palmira
Perez, Edith
Mohamed, Fabian Heber
author Mariqueo, Betsabé L.
author_facet Mariqueo, Betsabé L.
Diaz Guevara, María Clara
Rosales, Gabriel
Filippa, Veronica Palmira
Perez, Edith
Mohamed, Fabian Heber
author_role author
author2 Diaz Guevara, María Clara
Rosales, Gabriel
Filippa, Veronica Palmira
Perez, Edith
Mohamed, Fabian Heber
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LAGOSTOMUS
PITUITARY
S-100 PROTEIN
SEASONAL
topic LAGOSTOMUS
PITUITARY
S-100 PROTEIN
SEASONAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The pars nervosa (PN) of the neurohypophysis is mainly constituted by pituicytes and other neuroglial cells such as microglial cells, astrocytes and oligodendrocytes, endothelial cells of blood vessels and amylinic axons. The PN pituicytes express the S-100 protein that has a regulatory role within the cell and on different target cells. The aim of this work was to study the seasonal expression of S100 protein in pituitary PN of adult male viscachas (Lagostomus maximus maximus). The animals were captured in their habitat during the most representative months of their annual reproductive cycle. This cycle has three periods: reproductive (RepP), gonadal regression (RegP), and gonadal recovery (RecP). Four pituitary glands of each period were removed and processed for optical microscopy. The S-100 protein was used as markers of pituicytes and their expression was detected by immunohistochemistry. The primary antibody used was the rabbit polyclonal anti-S-100 protein. Immunohistochemical staining was performed using a streptavidinbiotin-peroxidase complex method with the 3, 3’- diaminobenzidine tetrahydrochloride (DAB) as chromogen. A morphometric study was performed and the percentage of immunopositive area for S-100 (% IA-S-100) was measured. The results obtained from these periods were statistically analyzed. Most of the pituicytes presented a cytoplamic immunostaining pattern. However, some of them also exhibited nuclear staining. The nuclei were round, oval and irregular in shape, with varying density of chromatin and an evident nucleolus. Numerous immunostained cytoplasmic processes in contact with blood vessels were observed. The % IA-S-100 varied throughout the annual reproductive cycle. The % IA during the RepP (13.09 ± 1.49) and RegP (9.77 ± 1.56) were significantly lower than in the RecP (25.61 ± 2.36; P < 0.001). These results demonstrated that the highest expression of S-100 protein was in the RecP. This period agrees with the increase of seasonal rainfall pattern during spring, so the viscachas have greater water availability in the environment. It is likely that the pituicytes participate in the seasonal regulation of the neurohormonal secretion through the expression of the S-100 protein. This protein is associated with functions such as modulation of enzymatic activity, stimulation of adenylate cyclase, maintenance of cell shape and mobility. However, further research is needed to elucidate the relationship between S-100 expression and the neurohormonal PN secretion of Lagostomus.
Fil: Mariqueo, Betsabé L.. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Diaz Guevara, María Clara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rosales, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina
Fil: Perez, Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Interuniversitario Nacional. - Ministerio de Educacion, Cultura, Ciencia y Tecnologia. Consejo Interuniversitario Nacional.; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias Biológicas
San Luis
Argentina
Sociedad de Biología de Cuyo
description The pars nervosa (PN) of the neurohypophysis is mainly constituted by pituicytes and other neuroglial cells such as microglial cells, astrocytes and oligodendrocytes, endothelial cells of blood vessels and amylinic axons. The PN pituicytes express the S-100 protein that has a regulatory role within the cell and on different target cells. The aim of this work was to study the seasonal expression of S100 protein in pituitary PN of adult male viscachas (Lagostomus maximus maximus). The animals were captured in their habitat during the most representative months of their annual reproductive cycle. This cycle has three periods: reproductive (RepP), gonadal regression (RegP), and gonadal recovery (RecP). Four pituitary glands of each period were removed and processed for optical microscopy. The S-100 protein was used as markers of pituicytes and their expression was detected by immunohistochemistry. The primary antibody used was the rabbit polyclonal anti-S-100 protein. Immunohistochemical staining was performed using a streptavidinbiotin-peroxidase complex method with the 3, 3’- diaminobenzidine tetrahydrochloride (DAB) as chromogen. A morphometric study was performed and the percentage of immunopositive area for S-100 (% IA-S-100) was measured. The results obtained from these periods were statistically analyzed. Most of the pituicytes presented a cytoplamic immunostaining pattern. However, some of them also exhibited nuclear staining. The nuclei were round, oval and irregular in shape, with varying density of chromatin and an evident nucleolus. Numerous immunostained cytoplasmic processes in contact with blood vessels were observed. The % IA-S-100 varied throughout the annual reproductive cycle. The % IA during the RepP (13.09 ± 1.49) and RegP (9.77 ± 1.56) were significantly lower than in the RecP (25.61 ± 2.36; P < 0.001). These results demonstrated that the highest expression of S-100 protein was in the RecP. This period agrees with the increase of seasonal rainfall pattern during spring, so the viscachas have greater water availability in the environment. It is likely that the pituicytes participate in the seasonal regulation of the neurohormonal secretion through the expression of the S-100 protein. This protein is associated with functions such as modulation of enzymatic activity, stimulation of adenylate cyclase, maintenance of cell shape and mobility. However, further research is needed to elucidate the relationship between S-100 expression and the neurohormonal PN secretion of Lagostomus.
publishDate 2020
dc.date.none.fl_str_mv 2020
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Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159993
Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus); XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias Biológicas; San Luis; Argentina; 2019; 61-61
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159993
identifier_str_mv Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus); XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo: Educación, Salud y ambiente: Los nuevos desafíos en las Ciencias Biológicas; San Luis; Argentina; 2019; 61-61
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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