The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions

Autores
Farizano, Juan Vicente; Pescaretti, María de Las Mercedes; Lopez, Fabian E.; Fong-Fu, Hsu; Delgado, Monica Alejandra
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Salmonella enterica serovar Typhimurium lipopolysaccharide consisting of covalently linked lipid A, non-repeating core oligosaccharide, and the O-antigen polysaccharide is the most exposed component of the cell envelope. Previous studies demonstrated that all of these regions act against the host immunity barrier. The aim of this study was to define the role and interaction of PmrAB-dependent gene products required for the lipopolysaccharide component synthesis or modification mainly during the Salmonella infection. The PmrAB two-component system activation promotes a remodeling of lipid A and the core region by addition of 4-aminoarabinose and/or phosphoethanolamine. These PmrA-dependent activities are produced by activation of ugd, pbgPE, pmrC, cpta, and pmrG transcription. In addition, under PmrA-regulator activation, the expression of wzzfepE and wzzst genes is induced, and their products are required to determine the O-antigen chain length. Here we report for the first time that Wzzst protein is necessary to maintain the balance of 4-aminoarabinose and phosphoethanolamine lipid A modifications. Moreover, we demonstrate that the interaction of the PmrA-dependent pbgE2 and pbgE3 gene products is important for the formation of the short O-antigen region. Our results establish that PmrAB is the global regulatory system that controls lipopolysaccharide modification, leading to a coordinate regulation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defense mechanisms.
Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fong-Fu, Hsu. Washington University in St. Louis; Estados Unidos
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Washington; Estados Unidos
Materia
Lps
O-Antigen
Salmonella
Virulence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61450
Acceder
id CONICETDig_6e97eac4a38930deb62aa8583d30b785
oai_identifier_str oai:ri.conicet.gov.ar:11336/61450
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host InteractionsFarizano, Juan VicentePescaretti, María de Las MercedesLopez, Fabian E.Fong-Fu, HsuDelgado, Monica AlejandraLpsO-AntigenSalmonellaVirulencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Salmonella enterica serovar Typhimurium lipopolysaccharide consisting of covalently linked lipid A, non-repeating core oligosaccharide, and the O-antigen polysaccharide is the most exposed component of the cell envelope. Previous studies demonstrated that all of these regions act against the host immunity barrier. The aim of this study was to define the role and interaction of PmrAB-dependent gene products required for the lipopolysaccharide component synthesis or modification mainly during the Salmonella infection. The PmrAB two-component system activation promotes a remodeling of lipid A and the core region by addition of 4-aminoarabinose and/or phosphoethanolamine. These PmrA-dependent activities are produced by activation of ugd, pbgPE, pmrC, cpta, and pmrG transcription. In addition, under PmrA-regulator activation, the expression of wzzfepE and wzzst genes is induced, and their products are required to determine the O-antigen chain length. Here we report for the first time that Wzzst protein is necessary to maintain the balance of 4-aminoarabinose and phosphoethanolamine lipid A modifications. Moreover, we demonstrate that the interaction of the PmrA-dependent pbgE2 and pbgE3 gene products is important for the formation of the short O-antigen region. Our results establish that PmrAB is the global regulatory system that controls lipopolysaccharide modification, leading to a coordinate regulation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defense mechanisms.Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Lopez, Fabian E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fong-Fu, Hsu. Washington University in St. Louis; Estados UnidosFil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Washington; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61450Farizano, Juan Vicente; Pescaretti, María de Las Mercedes; Lopez, Fabian E.; Fong-Fu, Hsu; Delgado, Monica Alejandra; The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 46; 11-2012; 38778-387890021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/46/38778info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M112.397414info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:04Zoai:ri.conicet.gov.ar:11336/61450instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:04.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
title The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
spellingShingle The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
Farizano, Juan Vicente
Lps
O-Antigen
Salmonella
Virulence
title_short The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
title_full The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
title_fullStr The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
title_full_unstemmed The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
title_sort The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions
dc.creator.none.fl_str_mv Farizano, Juan Vicente
Pescaretti, María de Las Mercedes
Lopez, Fabian E.
Fong-Fu, Hsu
Delgado, Monica Alejandra
author Farizano, Juan Vicente
author_facet Farizano, Juan Vicente
Pescaretti, María de Las Mercedes
Lopez, Fabian E.
Fong-Fu, Hsu
Delgado, Monica Alejandra
author_role author
author2 Pescaretti, María de Las Mercedes
Lopez, Fabian E.
Fong-Fu, Hsu
Delgado, Monica Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lps
O-Antigen
Salmonella
Virulence
topic Lps
O-Antigen
Salmonella
Virulence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Salmonella enterica serovar Typhimurium lipopolysaccharide consisting of covalently linked lipid A, non-repeating core oligosaccharide, and the O-antigen polysaccharide is the most exposed component of the cell envelope. Previous studies demonstrated that all of these regions act against the host immunity barrier. The aim of this study was to define the role and interaction of PmrAB-dependent gene products required for the lipopolysaccharide component synthesis or modification mainly during the Salmonella infection. The PmrAB two-component system activation promotes a remodeling of lipid A and the core region by addition of 4-aminoarabinose and/or phosphoethanolamine. These PmrA-dependent activities are produced by activation of ugd, pbgPE, pmrC, cpta, and pmrG transcription. In addition, under PmrA-regulator activation, the expression of wzzfepE and wzzst genes is induced, and their products are required to determine the O-antigen chain length. Here we report for the first time that Wzzst protein is necessary to maintain the balance of 4-aminoarabinose and phosphoethanolamine lipid A modifications. Moreover, we demonstrate that the interaction of the PmrA-dependent pbgE2 and pbgE3 gene products is important for the formation of the short O-antigen region. Our results establish that PmrAB is the global regulatory system that controls lipopolysaccharide modification, leading to a coordinate regulation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defense mechanisms.
Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lopez, Fabian E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fong-Fu, Hsu. Washington University in St. Louis; Estados Unidos
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. University of Washington; Estados Unidos
description The Salmonella enterica serovar Typhimurium lipopolysaccharide consisting of covalently linked lipid A, non-repeating core oligosaccharide, and the O-antigen polysaccharide is the most exposed component of the cell envelope. Previous studies demonstrated that all of these regions act against the host immunity barrier. The aim of this study was to define the role and interaction of PmrAB-dependent gene products required for the lipopolysaccharide component synthesis or modification mainly during the Salmonella infection. The PmrAB two-component system activation promotes a remodeling of lipid A and the core region by addition of 4-aminoarabinose and/or phosphoethanolamine. These PmrA-dependent activities are produced by activation of ugd, pbgPE, pmrC, cpta, and pmrG transcription. In addition, under PmrA-regulator activation, the expression of wzzfepE and wzzst genes is induced, and their products are required to determine the O-antigen chain length. Here we report for the first time that Wzzst protein is necessary to maintain the balance of 4-aminoarabinose and phosphoethanolamine lipid A modifications. Moreover, we demonstrate that the interaction of the PmrA-dependent pbgE2 and pbgE3 gene products is important for the formation of the short O-antigen region. Our results establish that PmrAB is the global regulatory system that controls lipopolysaccharide modification, leading to a coordinate regulation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defense mechanisms.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61450
Farizano, Juan Vicente; Pescaretti, María de Las Mercedes; Lopez, Fabian E.; Fong-Fu, Hsu; Delgado, Monica Alejandra; The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 46; 11-2012; 38778-38789
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61450
identifier_str_mv Farizano, Juan Vicente; Pescaretti, María de Las Mercedes; Lopez, Fabian E.; Fong-Fu, Hsu; Delgado, Monica Alejandra; The PmrAB System-inducing Conditions Control Both Lipid A Remodeling and O-antigen Length Distribution, Influencing the Salmonella Typhimurium-Host Interactions; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 46; 11-2012; 38778-38789
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/46/38778
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M112.397414
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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