Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons

Autores
Aparicio, Gabriela Inés; León, Antonella; Gutierrez Fuster, Rocio; Ravenscraft, Baylen; Monje, Paula; Scorticati, Camila
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.
Fil: Aparicio, Gabriela Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. University of Kentucky; Estados Unidos
Fil: León, Antonella. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Gutierrez Fuster, Rocio. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ravenscraft, Baylen. Indiana University. School Of Medicine.; Estados Unidos
Fil: Monje, Paula. University of Kentucky; Estados Unidos
Fil: Scorticati, Camila. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
DORSAL ROOT GANGLION NEURONS
GLYCOPROTEIN
GPM6A
NEURITOGENESIS
PERIPHERAL NERVOUS SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228709
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228709
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neuronsAparicio, Gabriela InésLeón, AntonellaGutierrez Fuster, RocioRavenscraft, BaylenMonje, PaulaScorticati, CamilaDORSAL ROOT GANGLION NEURONSGLYCOPROTEINGPM6ANEURITOGENESISPERIPHERAL NERVOUS SYSTEMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.Fil: Aparicio, Gabriela Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. University of Kentucky; Estados UnidosFil: León, Antonella. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Gutierrez Fuster, Rocio. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Ravenscraft, Baylen. Indiana University. School Of Medicine.; Estados UnidosFil: Monje, Paula. University of Kentucky; Estados UnidosFil: Scorticati, Camila. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaMultidisciplinary Digital Publishing Institute2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228709Aparicio, Gabriela Inés; León, Antonella; Gutierrez Fuster, Rocio; Ravenscraft, Baylen; Monje, Paula; et al.; Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons; Multidisciplinary Digital Publishing Institute; Biomolecules; 13; 4; 3-2023; 1-142218-273XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/13/4/594/review_reportinfo:eu-repo/semantics/altIdentifier/doi/10.3390/biom13040594info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T09:12:32Zoai:ri.conicet.gov.ar:11336/228709instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 09:12:32.769CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
title Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
spellingShingle Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
Aparicio, Gabriela Inés
DORSAL ROOT GANGLION NEURONS
GLYCOPROTEIN
GPM6A
NEURITOGENESIS
PERIPHERAL NERVOUS SYSTEM
title_short Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
title_full Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
title_fullStr Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
title_full_unstemmed Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
title_sort Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons
dc.creator.none.fl_str_mv Aparicio, Gabriela Inés
León, Antonella
Gutierrez Fuster, Rocio
Ravenscraft, Baylen
Monje, Paula
Scorticati, Camila
author Aparicio, Gabriela Inés
author_facet Aparicio, Gabriela Inés
León, Antonella
Gutierrez Fuster, Rocio
Ravenscraft, Baylen
Monje, Paula
Scorticati, Camila
author_role author
author2 León, Antonella
Gutierrez Fuster, Rocio
Ravenscraft, Baylen
Monje, Paula
Scorticati, Camila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DORSAL ROOT GANGLION NEURONS
GLYCOPROTEIN
GPM6A
NEURITOGENESIS
PERIPHERAL NERVOUS SYSTEM
topic DORSAL ROOT GANGLION NEURONS
GLYCOPROTEIN
GPM6A
NEURITOGENESIS
PERIPHERAL NERVOUS SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.
Fil: Aparicio, Gabriela Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. University of Kentucky; Estados Unidos
Fil: León, Antonella. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Gutierrez Fuster, Rocio. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ravenscraft, Baylen. Indiana University. School Of Medicine.; Estados Unidos
Fil: Monje, Paula. University of Kentucky; Estados Unidos
Fil: Scorticati, Camila. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
description The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public RNA-seq datasets with immunochemical approaches utilizing cultures of rat DRG explants and dissociated neuronal cells. M6a was detected on the cell surfaces of DRG neurons throughout development. Moreover, GPM6a was required for DRG neurite elongation in vitro. In summary, we provide evidence on GPM6a being present in DRG neurons for the first time. Data from our functional experiments support the idea that GPM6a could contribute to axon regeneration in the PNS.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228709
Aparicio, Gabriela Inés; León, Antonella; Gutierrez Fuster, Rocio; Ravenscraft, Baylen; Monje, Paula; et al.; Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons; Multidisciplinary Digital Publishing Institute; Biomolecules; 13; 4; 3-2023; 1-14
2218-273X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228709
identifier_str_mv Aparicio, Gabriela Inés; León, Antonella; Gutierrez Fuster, Rocio; Ravenscraft, Baylen; Monje, Paula; et al.; Endogenous glycoprotein M6a is involved in neurite outgrowth in rat dorsal root ganglion neurons; Multidisciplinary Digital Publishing Institute; Biomolecules; 13; 4; 3-2023; 1-14
2218-273X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-273X/13/4/594/review_report
info:eu-repo/semantics/altIdentifier/doi/10.3390/biom13040594
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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