Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development

Autores
Benitez, Sergio Gonzalo; Seltzer, Alicia Mabel; Acosta, Cristian
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
tAT2 receptor (AT2R) plays a functional role in foetal development. Its expression declines in most tissuessoon after birth but stays high in sensory areas of the adult nervous system. In the dorsal root ganglia (DRG)the expression pattern of AT2R during development and the identity of the subpopulation expressing itremain unknown. Using a combination of semi-quantitative PCR, western blotting and immunohisto-chemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonicday 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibitedonly minor (statistically non-significant) fluctuations from E15 to PN30. Detailed quantitative analysisof ABC/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 andb) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons areputative C-nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whetherthese AT2R-positive neurons co-localized with Ret and trkA embryonically and with IB4-binding post-natally. Most AT2R-positive neurons expressed trkA embryonically and bound IB4 postnatally. We foundstrong positive statistically highly significant correlations between AT2R cytoplasmic%intensities andtrkA at E15/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated withIB4-binding at PN3, 15 and 30. Our demonstration that a subpopulation of C-nociceptor-like neuronsexpresses AT2R during development supports a role for this receptor in neuropathic pain.
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Acosta, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Angiotensin-Ii
Ib4
Pain
Sensory Neurons
Trka
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48919
Acceder
id CONICETDig_3fbcbe23e6edef1e1fa117ef2d2ee2f9
oai_identifier_str oai:ri.conicet.gov.ar:11336/48919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout developmentBenitez, Sergio GonzaloSeltzer, Alicia MabelAcosta, CristianAngiotensin-IiIb4PainSensory NeuronsTrkahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1tAT2 receptor (AT2R) plays a functional role in foetal development. Its expression declines in most tissuessoon after birth but stays high in sensory areas of the adult nervous system. In the dorsal root ganglia (DRG)the expression pattern of AT2R during development and the identity of the subpopulation expressing itremain unknown. Using a combination of semi-quantitative PCR, western blotting and immunohisto-chemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonicday 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibitedonly minor (statistically non-significant) fluctuations from E15 to PN30. Detailed quantitative analysisof ABC/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 andb) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons areputative C-nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whetherthese AT2R-positive neurons co-localized with Ret and trkA embryonically and with IB4-binding post-natally. Most AT2R-positive neurons expressed trkA embryonically and bound IB4 postnatally. We foundstrong positive statistically highly significant correlations between AT2R cytoplasmic%intensities andtrkA at E15/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated withIB4-binding at PN3, 15 and 30. Our demonstration that a subpopulation of C-nociceptor-like neuronsexpresses AT2R during development supports a role for this receptor in neuropathic pain.Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Acosta, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaPergamon-Elsevier Science Ltd2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48919Benitez, Sergio Gonzalo; Seltzer, Alicia Mabel; Acosta, Cristian; Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 55; 9-2016; 10-170736-5748CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0736574816302891info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijdevneu.2016.11.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:36Zoai:ri.conicet.gov.ar:11336/48919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:37.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
title Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
spellingShingle Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
Benitez, Sergio Gonzalo
Angiotensin-Ii
Ib4
Pain
Sensory Neurons
Trka
title_short Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
title_full Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
title_fullStr Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
title_full_unstemmed Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
title_sort Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development
dc.creator.none.fl_str_mv Benitez, Sergio Gonzalo
Seltzer, Alicia Mabel
Acosta, Cristian
author Benitez, Sergio Gonzalo
author_facet Benitez, Sergio Gonzalo
Seltzer, Alicia Mabel
Acosta, Cristian
author_role author
author2 Seltzer, Alicia Mabel
Acosta, Cristian
author2_role author
author
dc.subject.none.fl_str_mv Angiotensin-Ii
Ib4
Pain
Sensory Neurons
Trka
topic Angiotensin-Ii
Ib4
Pain
Sensory Neurons
Trka
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv tAT2 receptor (AT2R) plays a functional role in foetal development. Its expression declines in most tissuessoon after birth but stays high in sensory areas of the adult nervous system. In the dorsal root ganglia (DRG)the expression pattern of AT2R during development and the identity of the subpopulation expressing itremain unknown. Using a combination of semi-quantitative PCR, western blotting and immunohisto-chemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonicday 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibitedonly minor (statistically non-significant) fluctuations from E15 to PN30. Detailed quantitative analysisof ABC/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 andb) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons areputative C-nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whetherthese AT2R-positive neurons co-localized with Ret and trkA embryonically and with IB4-binding post-natally. Most AT2R-positive neurons expressed trkA embryonically and bound IB4 postnatally. We foundstrong positive statistically highly significant correlations between AT2R cytoplasmic%intensities andtrkA at E15/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated withIB4-binding at PN3, 15 and 30. Our demonstration that a subpopulation of C-nociceptor-like neuronsexpresses AT2R during development supports a role for this receptor in neuropathic pain.
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Seltzer, Alicia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Acosta, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description tAT2 receptor (AT2R) plays a functional role in foetal development. Its expression declines in most tissuessoon after birth but stays high in sensory areas of the adult nervous system. In the dorsal root ganglia (DRG)the expression pattern of AT2R during development and the identity of the subpopulation expressing itremain unknown. Using a combination of semi-quantitative PCR, western blotting and immunohisto-chemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonicday 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibitedonly minor (statistically non-significant) fluctuations from E15 to PN30. Detailed quantitative analysisof ABC/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 andb) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons areputative C-nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whetherthese AT2R-positive neurons co-localized with Ret and trkA embryonically and with IB4-binding post-natally. Most AT2R-positive neurons expressed trkA embryonically and bound IB4 postnatally. We foundstrong positive statistically highly significant correlations between AT2R cytoplasmic%intensities andtrkA at E15/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated withIB4-binding at PN3, 15 and 30. Our demonstration that a subpopulation of C-nociceptor-like neuronsexpresses AT2R during development supports a role for this receptor in neuropathic pain.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48919
Benitez, Sergio Gonzalo; Seltzer, Alicia Mabel; Acosta, Cristian; Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 55; 9-2016; 10-17
0736-5748
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48919
identifier_str_mv Benitez, Sergio Gonzalo; Seltzer, Alicia Mabel; Acosta, Cristian; Nociceptor-like rat dorsal root ganglion neurons express theangiotensin-II AT2 receptor throughout development; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 55; 9-2016; 10-17
0736-5748
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0736574816302891
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijdevneu.2016.11.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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