Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology

Autores
Guevara, Palmira; Dias, Manuel; Rojas, Agustina; Crisante, Gladys; Abreu Blanco, Maria Teresa; Umezawa, Eufrozina; Vazquez, Martin Pablo; Levin, Mariano Jorge; Añez, Nestor; Ramirez, Jose Luis
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasites
Fil: Guevara, Palmira. Universidad Central de Venezuela; Venezuela
Fil: Dias, Manuel. Universidad de los Andes; Colombia
Fil: Rojas, Agustina. Universidad de los Andes; Colombia
Fil: Crisante, Gladys. Universidad de los Andes; Colombia
Fil: Abreu Blanco, Maria Teresa. Universidad Central de Venezuela; Venezuela
Fil: Umezawa, Eufrozina. Universidade de Sao Paulo; Brasil
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Añez, Nestor. Universidad de los Andes; Colombia
Fil: Ramirez, Jose Luis. Instituto de Estudios Avanzados; Venezuela
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79880
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79880
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector BiologyGuevara, PalmiraDias, ManuelRojas, AgustinaCrisante, GladysAbreu Blanco, Maria TeresaUmezawa, EufrozinaVazquez, Martin PabloLevin, Mariano JorgeAñez, NestorRamirez, Jose Luishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasitesFil: Guevara, Palmira. Universidad Central de Venezuela; VenezuelaFil: Dias, Manuel. Universidad de los Andes; ColombiaFil: Rojas, Agustina. Universidad de los Andes; ColombiaFil: Crisante, Gladys. Universidad de los Andes; ColombiaFil: Abreu Blanco, Maria Teresa. Universidad Central de Venezuela; VenezuelaFil: Umezawa, Eufrozina. Universidade de Sao Paulo; BrasilFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Añez, Nestor. Universidad de los Andes; ColombiaFil: Ramirez, Jose Luis. Instituto de Estudios Avanzados; VenezuelaOxford University Press2005-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79880Guevara, Palmira; Dias, Manuel; Rojas, Agustina; Crisante, Gladys; Abreu Blanco, Maria Teresa; et al.; Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology; Oxford University Press; Journal of Medical Entomology; 42; 1; 1-2005; 48-560022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15691008info:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/42.1.48info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/42/1/48/847455info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:35Zoai:ri.conicet.gov.ar:11336/79880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:35.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
title Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
spellingShingle Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
Guevara, Palmira
title_short Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
title_full Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
title_fullStr Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
title_full_unstemmed Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
title_sort Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology
dc.creator.none.fl_str_mv Guevara, Palmira
Dias, Manuel
Rojas, Agustina
Crisante, Gladys
Abreu Blanco, Maria Teresa
Umezawa, Eufrozina
Vazquez, Martin Pablo
Levin, Mariano Jorge
Añez, Nestor
Ramirez, Jose Luis
author Guevara, Palmira
author_facet Guevara, Palmira
Dias, Manuel
Rojas, Agustina
Crisante, Gladys
Abreu Blanco, Maria Teresa
Umezawa, Eufrozina
Vazquez, Martin Pablo
Levin, Mariano Jorge
Añez, Nestor
Ramirez, Jose Luis
author_role author
author2 Dias, Manuel
Rojas, Agustina
Crisante, Gladys
Abreu Blanco, Maria Teresa
Umezawa, Eufrozina
Vazquez, Martin Pablo
Levin, Mariano Jorge
Añez, Nestor
Ramirez, Jose Luis
author2_role author
author
author
author
author
author
author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasites
Fil: Guevara, Palmira. Universidad Central de Venezuela; Venezuela
Fil: Dias, Manuel. Universidad de los Andes; Colombia
Fil: Rojas, Agustina. Universidad de los Andes; Colombia
Fil: Crisante, Gladys. Universidad de los Andes; Colombia
Fil: Abreu Blanco, Maria Teresa. Universidad Central de Venezuela; Venezuela
Fil: Umezawa, Eufrozina. Universidade de Sao Paulo; Brasil
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Añez, Nestor. Universidad de los Andes; Colombia
Fil: Ramirez, Jose Luis. Instituto de Estudios Avanzados; Venezuela
description Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasites
publishDate 2005
dc.date.none.fl_str_mv 2005-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79880
Guevara, Palmira; Dias, Manuel; Rojas, Agustina; Crisante, Gladys; Abreu Blanco, Maria Teresa; et al.; Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology; Oxford University Press; Journal of Medical Entomology; 42; 1; 1-2005; 48-56
0022-2585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79880
identifier_str_mv Guevara, Palmira; Dias, Manuel; Rojas, Agustina; Crisante, Gladys; Abreu Blanco, Maria Teresa; et al.; Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology; Oxford University Press; Journal of Medical Entomology; 42; 1; 1-2005; 48-56
0022-2585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15691008
info:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/42.1.48
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/42/1/48/847455
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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