Revisiting the development of Trypanosoma rangeli in the vertebrate host

Autores
Santos, Luan Felipe; De Souza Rocha, Flávia; Lorenzo, Marcelo Gustavo; Guarneri, Alessandra Aparecida
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND Trypanosoma rangeli is a haemoflagellate parasite that infects triatomine bugs and mammals in South and Central America. Trypanosoma cruzi, the etiological agent of Chagas disease, has a partially overlapping geographical distribution with T. rangeli, that leads to mixed human infections and cross-reactivity in immunodiagnosis. Although T. rangeli can be detected long after mammal infection, its multiplicative forms have not yet been described.OBJECTIVES: To enhance our understanding of T. rangeli development in mammals, this study assessed various infection parameters in mice over time.METHODS: The parasitaemia, body temperature, and weight of Swiss Webster mice were monitored over 120 days after exposing them to the bites of Rhodnius prolixus nymphs containing metacyclic trypomastigotes in their salivary glands. On day 132 post-infection, spleens and mesenteric lymph nodes were analysed for T. rangeli DNA using polymerase chain reaction (PCR) and quantitative PCR (qPCR).FINDINGS: Parasites were detectable in mice blood since day 2 post-infection, detection peaking on day 5 and becoming undetectable by day 120. PCR and qPCR detected T. rangeli DNA in the spleens and mesenteric lymph nodes of infected mice. Infected mice showed higher body temperatures and a slower weight gain over time compared to controls.MAIN CONCLUSIONS: The study confirmed that T. rangeli establishes a persistent infection in mice, detectable in lymphoid organs long after parasites had disappeared from blood. In addition, infected mice exhibited physiological changes, suggesting potential subclinical effects. These findings highlight the need for further studies on the immune response and potential impacts of T. rangeli infection in mammalian hosts.
Fil: Santos, Luan Felipe. Instituto Oswaldo Cruz; Brasil
Fil: De Souza Rocha, Flávia. Instituto Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Guarneri, Alessandra Aparecida. Instituto Oswaldo Cruz; Brasil
Materia
Trypanosoma
Triatomines
Mice
Infection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263505
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263505
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Revisiting the development of Trypanosoma rangeli in the vertebrate hostSantos, Luan FelipeDe Souza Rocha, FláviaLorenzo, Marcelo GustavoGuarneri, Alessandra AparecidaTrypanosomaTriatominesMiceInfectionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND Trypanosoma rangeli is a haemoflagellate parasite that infects triatomine bugs and mammals in South and Central America. Trypanosoma cruzi, the etiological agent of Chagas disease, has a partially overlapping geographical distribution with T. rangeli, that leads to mixed human infections and cross-reactivity in immunodiagnosis. Although T. rangeli can be detected long after mammal infection, its multiplicative forms have not yet been described.OBJECTIVES: To enhance our understanding of T. rangeli development in mammals, this study assessed various infection parameters in mice over time.METHODS: The parasitaemia, body temperature, and weight of Swiss Webster mice were monitored over 120 days after exposing them to the bites of Rhodnius prolixus nymphs containing metacyclic trypomastigotes in their salivary glands. On day 132 post-infection, spleens and mesenteric lymph nodes were analysed for T. rangeli DNA using polymerase chain reaction (PCR) and quantitative PCR (qPCR).FINDINGS: Parasites were detectable in mice blood since day 2 post-infection, detection peaking on day 5 and becoming undetectable by day 120. PCR and qPCR detected T. rangeli DNA in the spleens and mesenteric lymph nodes of infected mice. Infected mice showed higher body temperatures and a slower weight gain over time compared to controls.MAIN CONCLUSIONS: The study confirmed that T. rangeli establishes a persistent infection in mice, detectable in lymphoid organs long after parasites had disappeared from blood. In addition, infected mice exhibited physiological changes, suggesting potential subclinical effects. These findings highlight the need for further studies on the immune response and potential impacts of T. rangeli infection in mammalian hosts.Fil: Santos, Luan Felipe. Instituto Oswaldo Cruz; BrasilFil: De Souza Rocha, Flávia. Instituto Oswaldo Cruz; BrasilFil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Guarneri, Alessandra Aparecida. Instituto Oswaldo Cruz; BrasilFundação Oswaldo Cruz2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263505Santos, Luan Felipe; De Souza Rocha, Flávia; Lorenzo, Marcelo Gustavo; Guarneri, Alessandra Aparecida; Revisiting the development of Trypanosoma rangeli in the vertebrate host; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 119; 10-2024; 1-70074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762024000101136&tlng=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760240138info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:03Zoai:ri.conicet.gov.ar:11336/263505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:03.737CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Revisiting the development of Trypanosoma rangeli in the vertebrate host
title Revisiting the development of Trypanosoma rangeli in the vertebrate host
spellingShingle Revisiting the development of Trypanosoma rangeli in the vertebrate host
Santos, Luan Felipe
Trypanosoma
Triatomines
Mice
Infection
title_short Revisiting the development of Trypanosoma rangeli in the vertebrate host
title_full Revisiting the development of Trypanosoma rangeli in the vertebrate host
title_fullStr Revisiting the development of Trypanosoma rangeli in the vertebrate host
title_full_unstemmed Revisiting the development of Trypanosoma rangeli in the vertebrate host
title_sort Revisiting the development of Trypanosoma rangeli in the vertebrate host
dc.creator.none.fl_str_mv Santos, Luan Felipe
De Souza Rocha, Flávia
Lorenzo, Marcelo Gustavo
Guarneri, Alessandra Aparecida
author Santos, Luan Felipe
author_facet Santos, Luan Felipe
De Souza Rocha, Flávia
Lorenzo, Marcelo Gustavo
Guarneri, Alessandra Aparecida
author_role author
author2 De Souza Rocha, Flávia
Lorenzo, Marcelo Gustavo
Guarneri, Alessandra Aparecida
author2_role author
author
author
dc.subject.none.fl_str_mv Trypanosoma
Triatomines
Mice
Infection
topic Trypanosoma
Triatomines
Mice
Infection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BACKGROUND Trypanosoma rangeli is a haemoflagellate parasite that infects triatomine bugs and mammals in South and Central America. Trypanosoma cruzi, the etiological agent of Chagas disease, has a partially overlapping geographical distribution with T. rangeli, that leads to mixed human infections and cross-reactivity in immunodiagnosis. Although T. rangeli can be detected long after mammal infection, its multiplicative forms have not yet been described.OBJECTIVES: To enhance our understanding of T. rangeli development in mammals, this study assessed various infection parameters in mice over time.METHODS: The parasitaemia, body temperature, and weight of Swiss Webster mice were monitored over 120 days after exposing them to the bites of Rhodnius prolixus nymphs containing metacyclic trypomastigotes in their salivary glands. On day 132 post-infection, spleens and mesenteric lymph nodes were analysed for T. rangeli DNA using polymerase chain reaction (PCR) and quantitative PCR (qPCR).FINDINGS: Parasites were detectable in mice blood since day 2 post-infection, detection peaking on day 5 and becoming undetectable by day 120. PCR and qPCR detected T. rangeli DNA in the spleens and mesenteric lymph nodes of infected mice. Infected mice showed higher body temperatures and a slower weight gain over time compared to controls.MAIN CONCLUSIONS: The study confirmed that T. rangeli establishes a persistent infection in mice, detectable in lymphoid organs long after parasites had disappeared from blood. In addition, infected mice exhibited physiological changes, suggesting potential subclinical effects. These findings highlight the need for further studies on the immune response and potential impacts of T. rangeli infection in mammalian hosts.
Fil: Santos, Luan Felipe. Instituto Oswaldo Cruz; Brasil
Fil: De Souza Rocha, Flávia. Instituto Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Guarneri, Alessandra Aparecida. Instituto Oswaldo Cruz; Brasil
description BACKGROUND Trypanosoma rangeli is a haemoflagellate parasite that infects triatomine bugs and mammals in South and Central America. Trypanosoma cruzi, the etiological agent of Chagas disease, has a partially overlapping geographical distribution with T. rangeli, that leads to mixed human infections and cross-reactivity in immunodiagnosis. Although T. rangeli can be detected long after mammal infection, its multiplicative forms have not yet been described.OBJECTIVES: To enhance our understanding of T. rangeli development in mammals, this study assessed various infection parameters in mice over time.METHODS: The parasitaemia, body temperature, and weight of Swiss Webster mice were monitored over 120 days after exposing them to the bites of Rhodnius prolixus nymphs containing metacyclic trypomastigotes in their salivary glands. On day 132 post-infection, spleens and mesenteric lymph nodes were analysed for T. rangeli DNA using polymerase chain reaction (PCR) and quantitative PCR (qPCR).FINDINGS: Parasites were detectable in mice blood since day 2 post-infection, detection peaking on day 5 and becoming undetectable by day 120. PCR and qPCR detected T. rangeli DNA in the spleens and mesenteric lymph nodes of infected mice. Infected mice showed higher body temperatures and a slower weight gain over time compared to controls.MAIN CONCLUSIONS: The study confirmed that T. rangeli establishes a persistent infection in mice, detectable in lymphoid organs long after parasites had disappeared from blood. In addition, infected mice exhibited physiological changes, suggesting potential subclinical effects. These findings highlight the need for further studies on the immune response and potential impacts of T. rangeli infection in mammalian hosts.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263505
Santos, Luan Felipe; De Souza Rocha, Flávia; Lorenzo, Marcelo Gustavo; Guarneri, Alessandra Aparecida; Revisiting the development of Trypanosoma rangeli in the vertebrate host; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 119; 10-2024; 1-7
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263505
identifier_str_mv Santos, Luan Felipe; De Souza Rocha, Flávia; Lorenzo, Marcelo Gustavo; Guarneri, Alessandra Aparecida; Revisiting the development of Trypanosoma rangeli in the vertebrate host; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 119; 10-2024; 1-7
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762024000101136&tlng=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-02760240138
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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