Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014

Autores
Mandile, Marcelo Gastón; Argüelles, Marcelo Horacio; Temprana, Carlos Facundo; Peri Ibañez, Estefania Soledad; Silvestre, Dalila; Musto, Alejandra Beatriz; Rodríguez Pérez, Alberto; Mistchenko, Alicia Susana; Almallo de Glikmann, Graciela; Castello, Alejandro Andrés
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Group A rotaviruses (RVA) are the most frequent etiological agents causing severe diarrhea in infants and surveillance of genotype, and genetic characteristics of circulating strains are necessary in order to evaluate vaccine programs. The objectives of this work were to describe G and P genotype from 2012 through 2014 in Buenos Aires, Argentina completing an overview of 19 years of genotype surveillance in our region and to characterize an emerging G1P[8] strain associated with severe cases and five fatalities in 2014. We performed genotyping by RT-PCR. The sequencing of several genes, phylogenetic analyses, and comparative epidemiological data were used to know the origin and phylogenetic relationships of the emerging G1P[8] strain. Along with this report, 19 years of continuous RVA genotype surveillance in Argentina in the pre-vaccine era was covered. During the last year of this surveillance, 2014, a significantly increased incidence of RVA associated gastroenteritis was related to the reemergence of G1P[8] strains, being these ones detected in low frequency in the last nine years. Interestingly, the patients affected were significantly older when compared with those from the last six seasons. Additionally, phylogenetic analysis of several genes infer that these G1P[8] strains were closely related to Asian strains circulating during 2012 and 2013. In addition to this, the suggested extra continental origin for the 2014 G1P[8] strains and the very low circulation of G1 type during nine years probably explain the increased incidence and severity in the gastroenteritis cases and the particular epidemiologic characteristics. In conclusion, this work gives us a whole panorama of the pre-vaccine era of the RVA molecular epidemiology in the most populated region of Argentina. In this way, this work inspires us to continue with this type of studies in the post-vaccination era.
Fil: Mandile, Marcelo Gastón. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüelles, Marcelo Horacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peri Ibañez, Estefania Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvestre, Dalila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Musto, Alejandra Beatriz. Gobierno de la Provincia de Buenos Aires. Hospital Provincial Evita Pueblo.; Argentina
Fil: Rodríguez Pérez, Alberto. Hospital General de Agudos Dr. Alberto A. Eurnekian; Argentina
Fil: Mistchenko, Alicia Susana. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina
Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Universidad Nacional Arturo Jauretche; Argentina
Materia
ARGENTINA
PHYLOGENY
REEMERGENCE
ROTAVIRUS
VACCINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146176
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014Mandile, Marcelo GastónArgüelles, Marcelo HoracioTemprana, Carlos FacundoPeri Ibañez, Estefania SoledadSilvestre, DalilaMusto, Alejandra BeatrizRodríguez Pérez, AlbertoMistchenko, Alicia SusanaAlmallo de Glikmann, GracielaCastello, Alejandro AndrésARGENTINAPHYLOGENYREEMERGENCEROTAVIRUSVACCINEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Group A rotaviruses (RVA) are the most frequent etiological agents causing severe diarrhea in infants and surveillance of genotype, and genetic characteristics of circulating strains are necessary in order to evaluate vaccine programs. The objectives of this work were to describe G and P genotype from 2012 through 2014 in Buenos Aires, Argentina completing an overview of 19 years of genotype surveillance in our region and to characterize an emerging G1P[8] strain associated with severe cases and five fatalities in 2014. We performed genotyping by RT-PCR. The sequencing of several genes, phylogenetic analyses, and comparative epidemiological data were used to know the origin and phylogenetic relationships of the emerging G1P[8] strain. Along with this report, 19 years of continuous RVA genotype surveillance in Argentina in the pre-vaccine era was covered. During the last year of this surveillance, 2014, a significantly increased incidence of RVA associated gastroenteritis was related to the reemergence of G1P[8] strains, being these ones detected in low frequency in the last nine years. Interestingly, the patients affected were significantly older when compared with those from the last six seasons. Additionally, phylogenetic analysis of several genes infer that these G1P[8] strains were closely related to Asian strains circulating during 2012 and 2013. In addition to this, the suggested extra continental origin for the 2014 G1P[8] strains and the very low circulation of G1 type during nine years probably explain the increased incidence and severity in the gastroenteritis cases and the particular epidemiologic characteristics. In conclusion, this work gives us a whole panorama of the pre-vaccine era of the RVA molecular epidemiology in the most populated region of Argentina. In this way, this work inspires us to continue with this type of studies in the post-vaccination era.Fil: Mandile, Marcelo Gastón. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argüelles, Marcelo Horacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peri Ibañez, Estefania Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silvestre, Dalila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Musto, Alejandra Beatriz. Gobierno de la Provincia de Buenos Aires. Hospital Provincial Evita Pueblo.; ArgentinaFil: Rodríguez Pérez, Alberto. Hospital General de Agudos Dr. Alberto A. Eurnekian; ArgentinaFil: Mistchenko, Alicia Susana. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; ArgentinaFil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Universidad Nacional Arturo Jauretche; ArgentinaElsevier Science2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146176Mandile, Marcelo Gastón; Argüelles, Marcelo Horacio; Temprana, Carlos Facundo; Peri Ibañez, Estefania Soledad; Silvestre, Dalila; et al.; Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014; Elsevier Science; Infection, Genetics and Evolution; 80; 104192; 6-2020; 1-91567-13481567-7257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134820300241?via%3Dihub#!info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2020.104192info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:39Zoai:ri.conicet.gov.ar:11336/146176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:39.413CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
title Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
spellingShingle Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
Mandile, Marcelo Gastón
ARGENTINA
PHYLOGENY
REEMERGENCE
ROTAVIRUS
VACCINES
title_short Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
title_full Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
title_fullStr Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
title_full_unstemmed Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
title_sort Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014
dc.creator.none.fl_str_mv Mandile, Marcelo Gastón
Argüelles, Marcelo Horacio
Temprana, Carlos Facundo
Peri Ibañez, Estefania Soledad
Silvestre, Dalila
Musto, Alejandra Beatriz
Rodríguez Pérez, Alberto
Mistchenko, Alicia Susana
Almallo de Glikmann, Graciela
Castello, Alejandro Andrés
author Mandile, Marcelo Gastón
author_facet Mandile, Marcelo Gastón
Argüelles, Marcelo Horacio
Temprana, Carlos Facundo
Peri Ibañez, Estefania Soledad
Silvestre, Dalila
Musto, Alejandra Beatriz
Rodríguez Pérez, Alberto
Mistchenko, Alicia Susana
Almallo de Glikmann, Graciela
Castello, Alejandro Andrés
author_role author
author2 Argüelles, Marcelo Horacio
Temprana, Carlos Facundo
Peri Ibañez, Estefania Soledad
Silvestre, Dalila
Musto, Alejandra Beatriz
Rodríguez Pérez, Alberto
Mistchenko, Alicia Susana
Almallo de Glikmann, Graciela
Castello, Alejandro Andrés
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
PHYLOGENY
REEMERGENCE
ROTAVIRUS
VACCINES
topic ARGENTINA
PHYLOGENY
REEMERGENCE
ROTAVIRUS
VACCINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Group A rotaviruses (RVA) are the most frequent etiological agents causing severe diarrhea in infants and surveillance of genotype, and genetic characteristics of circulating strains are necessary in order to evaluate vaccine programs. The objectives of this work were to describe G and P genotype from 2012 through 2014 in Buenos Aires, Argentina completing an overview of 19 years of genotype surveillance in our region and to characterize an emerging G1P[8] strain associated with severe cases and five fatalities in 2014. We performed genotyping by RT-PCR. The sequencing of several genes, phylogenetic analyses, and comparative epidemiological data were used to know the origin and phylogenetic relationships of the emerging G1P[8] strain. Along with this report, 19 years of continuous RVA genotype surveillance in Argentina in the pre-vaccine era was covered. During the last year of this surveillance, 2014, a significantly increased incidence of RVA associated gastroenteritis was related to the reemergence of G1P[8] strains, being these ones detected in low frequency in the last nine years. Interestingly, the patients affected were significantly older when compared with those from the last six seasons. Additionally, phylogenetic analysis of several genes infer that these G1P[8] strains were closely related to Asian strains circulating during 2012 and 2013. In addition to this, the suggested extra continental origin for the 2014 G1P[8] strains and the very low circulation of G1 type during nine years probably explain the increased incidence and severity in the gastroenteritis cases and the particular epidemiologic characteristics. In conclusion, this work gives us a whole panorama of the pre-vaccine era of the RVA molecular epidemiology in the most populated region of Argentina. In this way, this work inspires us to continue with this type of studies in the post-vaccination era.
Fil: Mandile, Marcelo Gastón. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argüelles, Marcelo Horacio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peri Ibañez, Estefania Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvestre, Dalila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Musto, Alejandra Beatriz. Gobierno de la Provincia de Buenos Aires. Hospital Provincial Evita Pueblo.; Argentina
Fil: Rodríguez Pérez, Alberto. Hospital General de Agudos Dr. Alberto A. Eurnekian; Argentina
Fil: Mistchenko, Alicia Susana. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Departamento de Medicina; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina
Fil: Castello, Alejandro Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Universidad Nacional Arturo Jauretche; Argentina
description Group A rotaviruses (RVA) are the most frequent etiological agents causing severe diarrhea in infants and surveillance of genotype, and genetic characteristics of circulating strains are necessary in order to evaluate vaccine programs. The objectives of this work were to describe G and P genotype from 2012 through 2014 in Buenos Aires, Argentina completing an overview of 19 years of genotype surveillance in our region and to characterize an emerging G1P[8] strain associated with severe cases and five fatalities in 2014. We performed genotyping by RT-PCR. The sequencing of several genes, phylogenetic analyses, and comparative epidemiological data were used to know the origin and phylogenetic relationships of the emerging G1P[8] strain. Along with this report, 19 years of continuous RVA genotype surveillance in Argentina in the pre-vaccine era was covered. During the last year of this surveillance, 2014, a significantly increased incidence of RVA associated gastroenteritis was related to the reemergence of G1P[8] strains, being these ones detected in low frequency in the last nine years. Interestingly, the patients affected were significantly older when compared with those from the last six seasons. Additionally, phylogenetic analysis of several genes infer that these G1P[8] strains were closely related to Asian strains circulating during 2012 and 2013. In addition to this, the suggested extra continental origin for the 2014 G1P[8] strains and the very low circulation of G1 type during nine years probably explain the increased incidence and severity in the gastroenteritis cases and the particular epidemiologic characteristics. In conclusion, this work gives us a whole panorama of the pre-vaccine era of the RVA molecular epidemiology in the most populated region of Argentina. In this way, this work inspires us to continue with this type of studies in the post-vaccination era.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146176
Mandile, Marcelo Gastón; Argüelles, Marcelo Horacio; Temprana, Carlos Facundo; Peri Ibañez, Estefania Soledad; Silvestre, Dalila; et al.; Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014; Elsevier Science; Infection, Genetics and Evolution; 80; 104192; 6-2020; 1-9
1567-1348
1567-7257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146176
identifier_str_mv Mandile, Marcelo Gastón; Argüelles, Marcelo Horacio; Temprana, Carlos Facundo; Peri Ibañez, Estefania Soledad; Silvestre, Dalila; et al.; Pre-vaccine rotavirus surveillance in Buenos Aires, Argentina: Characterization of an emergent G1P[8] strain associated to fatal cases in 2014; Elsevier Science; Infection, Genetics and Evolution; 80; 104192; 6-2020; 1-9
1567-1348
1567-7257
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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