Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts

Autores
Morcillo, Nora; Imperiale, Belen Rocio; Di Giulio, Angela Beatríz; Zumarraga, Martin Jose; Takif, Howard; Cataldi, Angel Adrian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
Instituto de Biotecnología
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Takif, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Tuberculosis 94 (6) : 672-677 (Diciembre 2014)
Materia
Drug Resistance
Vigour
Households
Resistencia a Medicamentos
Tuberculosis
Vigor
Hogares
Mycobacterium tuberculosis
Family Transmission
Transmisión Familiar
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8191
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8191
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network_name_str INTA Digital (INTA)
spelling Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contactsMorcillo, NoraImperiale, Belen RocioDi Giulio, Angela BeatrízZumarraga, Martin JoseTakif, HowardCataldi, Angel AdrianDrug ResistanceVigourHouseholdsResistencia a MedicamentosTuberculosisVigorHogaresMycobacterium tuberculosisFamily TransmissionTransmisión FamiliarThere has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.Instituto de BiotecnologíaFil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaFil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaFil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Takif, Howard. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaElsevier2020-11-04T17:10:05Z2020-11-04T17:10:05Z2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8191https://www.sciencedirect.com/science/article/abs/pii/S14729792142039081472-9792https://doi.org/10.1016/j.tube.2014.08.003Tuberculosis 94 (6) : 672-677 (Diciembre 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:24Zoai:localhost:20.500.12123/8191instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:25.263INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
spellingShingle Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
Morcillo, Nora
Drug Resistance
Vigour
Households
Resistencia a Medicamentos
Tuberculosis
Vigor
Hogares
Mycobacterium tuberculosis
Family Transmission
Transmisión Familiar
title_short Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_full Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_fullStr Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_full_unstemmed Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
title_sort Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
dc.creator.none.fl_str_mv Morcillo, Nora
Imperiale, Belen Rocio
Di Giulio, Angela Beatríz
Zumarraga, Martin Jose
Takif, Howard
Cataldi, Angel Adrian
author Morcillo, Nora
author_facet Morcillo, Nora
Imperiale, Belen Rocio
Di Giulio, Angela Beatríz
Zumarraga, Martin Jose
Takif, Howard
Cataldi, Angel Adrian
author_role author
author2 Imperiale, Belen Rocio
Di Giulio, Angela Beatríz
Zumarraga, Martin Jose
Takif, Howard
Cataldi, Angel Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Drug Resistance
Vigour
Households
Resistencia a Medicamentos
Tuberculosis
Vigor
Hogares
Mycobacterium tuberculosis
Family Transmission
Transmisión Familiar
topic Drug Resistance
Vigour
Households
Resistencia a Medicamentos
Tuberculosis
Vigor
Hogares
Mycobacterium tuberculosis
Family Transmission
Transmisión Familiar
dc.description.none.fl_txt_mv There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
Instituto de Biotecnología
Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Takif, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
2020-11-04T17:10:05Z
2020-11-04T17:10:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8191
https://www.sciencedirect.com/science/article/abs/pii/S1472979214203908
1472-9792
https://doi.org/10.1016/j.tube.2014.08.003
url http://hdl.handle.net/20.500.12123/8191
https://www.sciencedirect.com/science/article/abs/pii/S1472979214203908
https://doi.org/10.1016/j.tube.2014.08.003
identifier_str_mv 1472-9792
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Tuberculosis 94 (6) : 672-677 (Diciembre 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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