Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli

Autores
Tomat, David Damian; Soazo, Marina del Valle; Verdini, Roxana Andrea; Casabonne, Cecilia; Aquili, Virginia Daniela; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted in the whey protein concentrate (WPC) matrix over 5 weeks of storage. In addition, the phages incorporated in the WPC matrix were characterized by their release from films into an aqueous system and solid food, as well as by their antimicrobial activity against pathogenic E. coli in a liquid medium and in meat at refrigerated, room and abusive temperatures. The results demonstrate that phages are highly stable in WPC films within 5 weeks of storage. Moreover, WPC films can release a significant number of phages into an aqueous system and onto a meat surface at similar concentrations. Phage-added films produced a significant reduction in E. coli cells to non-detectable levels in antimicrobial assays. Similar results, such as a complete inactivation achieved for DH5α and O157:H7 STEC strains, were obtained when antimicrobial assays were performed on meat. As a conclusion, the six-phage cocktail added into WPC films was highly stable, effectively released from films and proved highly effective as a biocontrol tool, though not under all the conditions evaluated.
Fil: Tomat, David Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Soazo, Marina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Verdini, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Casabonne, Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Aquili, Virginia Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
EDIBLE FILM
ESCHERICHIA COLI
PHAGE COCKTAIL
WHEY PROTEIN CONCENTRATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114078
Acceder
id CONICETDig_6cf32c957a4d1be3eb66ce3085cf46ab
oai_identifier_str oai:ri.conicet.gov.ar:11336/114078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coliTomat, David DamianSoazo, Marina del ValleVerdini, Roxana AndreaCasabonne, CeciliaAquili, Virginia DanielaBalague, Claudia ElisabehtQuiberoni, Andrea del LujanEDIBLE FILMESCHERICHIA COLIPHAGE COCKTAILWHEY PROTEIN CONCENTRATEhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted in the whey protein concentrate (WPC) matrix over 5 weeks of storage. In addition, the phages incorporated in the WPC matrix were characterized by their release from films into an aqueous system and solid food, as well as by their antimicrobial activity against pathogenic E. coli in a liquid medium and in meat at refrigerated, room and abusive temperatures. The results demonstrate that phages are highly stable in WPC films within 5 weeks of storage. Moreover, WPC films can release a significant number of phages into an aqueous system and onto a meat surface at similar concentrations. Phage-added films produced a significant reduction in E. coli cells to non-detectable levels in antimicrobial assays. Similar results, such as a complete inactivation achieved for DH5α and O157:H7 STEC strains, were obtained when antimicrobial assays were performed on meat. As a conclusion, the six-phage cocktail added into WPC films was highly stable, effectively released from films and proved highly effective as a biocontrol tool, though not under all the conditions evaluated.Fil: Tomat, David Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Soazo, Marina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Verdini, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Casabonne, Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Aquili, Virginia Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaElsevier Science2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114078Tomat, David Damian; Soazo, Marina del Valle; Verdini, Roxana Andrea; Casabonne, Cecilia; Aquili, Virginia Daniela; et al.; Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli; Elsevier Science; LWT - Food Science and Technology; 113; 10-20190023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643819306565info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108316info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:09Zoai:ri.conicet.gov.ar:11336/114078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:09.513CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
title Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
spellingShingle Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
Tomat, David Damian
EDIBLE FILM
ESCHERICHIA COLI
PHAGE COCKTAIL
WHEY PROTEIN CONCENTRATE
title_short Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
title_full Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
title_fullStr Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
title_full_unstemmed Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
title_sort Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
dc.creator.none.fl_str_mv Tomat, David Damian
Soazo, Marina del Valle
Verdini, Roxana Andrea
Casabonne, Cecilia
Aquili, Virginia Daniela
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author Tomat, David Damian
author_facet Tomat, David Damian
Soazo, Marina del Valle
Verdini, Roxana Andrea
Casabonne, Cecilia
Aquili, Virginia Daniela
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author_role author
author2 Soazo, Marina del Valle
Verdini, Roxana Andrea
Casabonne, Cecilia
Aquili, Virginia Daniela
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EDIBLE FILM
ESCHERICHIA COLI
PHAGE COCKTAIL
WHEY PROTEIN CONCENTRATE
topic EDIBLE FILM
ESCHERICHIA COLI
PHAGE COCKTAIL
WHEY PROTEIN CONCENTRATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted in the whey protein concentrate (WPC) matrix over 5 weeks of storage. In addition, the phages incorporated in the WPC matrix were characterized by their release from films into an aqueous system and solid food, as well as by their antimicrobial activity against pathogenic E. coli in a liquid medium and in meat at refrigerated, room and abusive temperatures. The results demonstrate that phages are highly stable in WPC films within 5 weeks of storage. Moreover, WPC films can release a significant number of phages into an aqueous system and onto a meat surface at similar concentrations. Phage-added films produced a significant reduction in E. coli cells to non-detectable levels in antimicrobial assays. Similar results, such as a complete inactivation achieved for DH5α and O157:H7 STEC strains, were obtained when antimicrobial assays were performed on meat. As a conclusion, the six-phage cocktail added into WPC films was highly stable, effectively released from films and proved highly effective as a biocontrol tool, though not under all the conditions evaluated.
Fil: Tomat, David Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Soazo, Marina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Verdini, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Casabonne, Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Aquili, Virginia Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted in the whey protein concentrate (WPC) matrix over 5 weeks of storage. In addition, the phages incorporated in the WPC matrix were characterized by their release from films into an aqueous system and solid food, as well as by their antimicrobial activity against pathogenic E. coli in a liquid medium and in meat at refrigerated, room and abusive temperatures. The results demonstrate that phages are highly stable in WPC films within 5 weeks of storage. Moreover, WPC films can release a significant number of phages into an aqueous system and onto a meat surface at similar concentrations. Phage-added films produced a significant reduction in E. coli cells to non-detectable levels in antimicrobial assays. Similar results, such as a complete inactivation achieved for DH5α and O157:H7 STEC strains, were obtained when antimicrobial assays were performed on meat. As a conclusion, the six-phage cocktail added into WPC films was highly stable, effectively released from films and proved highly effective as a biocontrol tool, though not under all the conditions evaluated.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114078
Tomat, David Damian; Soazo, Marina del Valle; Verdini, Roxana Andrea; Casabonne, Cecilia; Aquili, Virginia Daniela; et al.; Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli; Elsevier Science; LWT - Food Science and Technology; 113; 10-2019
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114078
identifier_str_mv Tomat, David Damian; Soazo, Marina del Valle; Verdini, Roxana Andrea; Casabonne, Cecilia; Aquili, Virginia Daniela; et al.; Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli; Elsevier Science; LWT - Food Science and Technology; 113; 10-2019
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643819306565
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108316
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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