Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation

Autores
Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.
Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercanti, Diego Javier. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quiberoni, Andrea del Lujan. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Escherichia Coli O157:H7 Stec
Bacteriophage
Phage Biocontrol
Milk Fermentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21570
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21570
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentationTomat, David DamianMercanti, Diego JavierBalague, Claudia ElisabehtQuiberoni, Andrea del LujanEscherichia Coli O157:H7 StecBacteriophagePhage BiocontrolMilk Fermentationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mercanti, Diego Javier. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Quiberoni, Andrea del Lujan. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21570Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan; Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation; Wiley; Letters In Applied Microbiology; 57; 1; 4-2013; 3-100266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/lam.12074info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/lam.12074/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:00Zoai:ri.conicet.gov.ar:11336/21570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:00.998CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
title Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
spellingShingle Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
Tomat, David Damian
Escherichia Coli O157:H7 Stec
Bacteriophage
Phage Biocontrol
Milk Fermentation
title_short Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
title_full Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
title_fullStr Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
title_full_unstemmed Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
title_sort Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
dc.creator.none.fl_str_mv Tomat, David Damian
Mercanti, Diego Javier
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author Tomat, David Damian
author_facet Tomat, David Damian
Mercanti, Diego Javier
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author_role author
author2 Mercanti, Diego Javier
Balague, Claudia Elisabeht
Quiberoni, Andrea del Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv Escherichia Coli O157:H7 Stec
Bacteriophage
Phage Biocontrol
Milk Fermentation
topic Escherichia Coli O157:H7 Stec
Bacteriophage
Phage Biocontrol
Milk Fermentation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.
Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercanti, Diego Javier. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quiberoni, Andrea del Lujan. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21570
Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan; Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation; Wiley; Letters In Applied Microbiology; 57; 1; 4-2013; 3-10
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21570
identifier_str_mv Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan; Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation; Wiley; Letters In Applied Microbiology; 57; 1; 4-2013; 3-10
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/lam.12074
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/lam.12074/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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